ID   M2DH_ASPCL              Reviewed;         502 AA.
AC   A1CLZ5;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Mannitol 2-dehydrogenase;
DE            Short=M2DH;
DE            Short=MDH;
DE            EC=1.1.1.67;
GN   ORFNames=ACLA_078730;
OS   Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS   NRRL 1 / QM 1276 / 107).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=344612;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Catalyzes the NAD(H)-dependent interconversion of D-fructose
CC       and D-mannitol in the mannitol metabolic pathway. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannitol + NAD(+) = D-fructose + H(+) + NADH;
CC         Xref=Rhea:RHEA:12084, ChEBI:CHEBI:15378, ChEBI:CHEBI:16899,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.67;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the mannitol dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; DS027057; EAW09124.1; -; Genomic_DNA.
DR   RefSeq; XP_001270550.1; XM_001270549.1.
DR   AlphaFoldDB; A1CLZ5; -.
DR   SMR; A1CLZ5; -.
DR   STRING; 5057.CADACLAP00007234; -.
DR   EnsemblFungi; EAW09124; EAW09124; ACLA_078730.
DR   GeneID; 4702636; -.
DR   KEGG; act:ACLA_078730; -.
DR   VEuPathDB; FungiDB:ACLA_078730; -.
DR   eggNOG; ENOG502QT30; Eukaryota.
DR   HOGENOM; CLU_027324_0_1_1; -.
DR   OMA; YKPYDNL; -.
DR   OrthoDB; 1095102at2759; -.
DR   Proteomes; UP000006701; Unassembled WGS sequence.
DR   GO; GO:0050086; F:mannitol 2-dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR000669; Mannitol_DH.
DR   InterPro; IPR013118; Mannitol_DH_C.
DR   InterPro; IPR013131; Mannitol_DH_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01232; Mannitol_dh; 1.
DR   Pfam; PF08125; Mannitol_dh_C; 1.
DR   PRINTS; PR00084; MTLDHDRGNASE.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..502
FT                   /note="Mannitol 2-dehydrogenase"
FT                   /id="PRO_0000371538"
FT   BINDING         37..48
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   502 AA;  56505 MW;  DEFE364514BACF0F CRC64;
     MAPLKLNSRN LSQIFAAGKS LVKVPTYQRN GAVKEGIVHI GVGGFHRAHL AVYVDRLMQE
     QGVTDYAICG VGLQPFDSTM RDALASQDNL YTVIERSAKG SFAHVIGSIN SYLFAPDDRE
     AVIAKMANPD TRIVSLTITE SGYYYNENTH ELQKEHPDIQ FDLNPVNENK PRTTFGFLYA
     ALERRHRLGL KPFTVMSCDN MQKNGSITRH MLESFARQRN PKVAEWIAEE GAFPNAMVDR
     ITPQTSATDK TALAENFGIE DSWPVVTEPF MQWVIEDKFS DGRPPFEKIG VQVVKDVHAV
     EQFEKHKLRL LNGSHSAIGY PGQLAGFNYV HEVMEHPLFS KFVWQMMQQE VKPLLPEIPG
     VNIDDYCNTL IERFTNPTIM DQLPRICLNA SGKIPQFIMP SIAEAIWVTA PFRRLCFVAA
     AWFCYIKGID DSGKAFEVVD PMLNELQAKA RAGGTNPSEL LSIKNLFGDD LRTDPRFLKE
     VTKAMEDITR DGILKTLPKY ID