M2K1_ORYSJ
ID M2K1_ORYSJ Reviewed; 355 AA.
AC Q5QN75; Q0JMF0; Q9FQM4;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Mitogen-activated protein kinase kinase 1;
DE Short=MAP kinase kinase 1;
DE Short=MAPKK1;
DE EC=2.7.12.2;
DE AltName: Full=OsMEK1;
GN Name=MKK1; Synonyms=MEK1; OrderedLocusNames=Os01g0510100, LOC_Os01g32660;
GN ORFNames=P0455H03.45, P0520B06.8;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MPK5/MAPK5, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=12177502; DOI=10.1104/pp.006072;
RA Wen J.-Q., Oono K., Imai R.;
RT "Two novel mitogen-activated protein signaling components, OsMEK1 and
RT OsMAP1, are involved in a moderate low-temperature signaling pathway in
RT rice.";
RL Plant Physiol. 129:1880-1891(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.12.2;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.12.2;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.12.2;
CC -!- ACTIVITY REGULATION: Activated through serine and threonine
CC phosphorylation. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with MPK5/MAPK5. {ECO:0000269|PubMed:12177502}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf sheaths and panicles.
CC {ECO:0000269|PubMed:12177502}.
CC -!- INDUCTION: By low temperature (12 degrees Celsius) treatment.
CC {ECO:0000269|PubMed:12177502}.
CC -!- PTM: Phosphorylated on Ser-221 and Thr-227, which activates the enzyme.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; AF216314; AAG40578.1; -; mRNA.
DR EMBL; AP003077; BAD73135.1; -; Genomic_DNA.
DR EMBL; AP003435; BAD73553.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05078.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72372.1; -; Genomic_DNA.
DR RefSeq; XP_015621394.1; XM_015765908.1.
DR AlphaFoldDB; Q5QN75; -.
DR SMR; Q5QN75; -.
DR STRING; 4530.OS01T0510100-01; -.
DR PaxDb; Q5QN75; -.
DR PRIDE; Q5QN75; -.
DR EnsemblPlants; Os01t0510100-01; Os01t0510100-01; Os01g0510100.
DR GeneID; 4324023; -.
DR Gramene; Os01t0510100-01; Os01t0510100-01; Os01g0510100.
DR KEGG; osa:4324023; -.
DR eggNOG; KOG0581; Eukaryota.
DR HOGENOM; CLU_000288_63_23_1; -.
DR InParanoid; Q5QN75; -.
DR OMA; YTVQFYG; -.
DR OrthoDB; 688282at2759; -.
DR BRENDA; 2.7.12.2; 4460.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5QN75; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0009524; C:phragmoplast; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004708; F:MAP kinase kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0051019; F:mitogen-activated protein kinase binding; IEA:EnsemblPlants.
DR GO; GO:0031435; F:mitogen-activated protein kinase kinase kinase binding; IEA:EnsemblPlants.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IEA:RHEA.
DR GO; GO:0002229; P:defense response to oomycetes; IEA:EnsemblPlants.
DR GO; GO:0010311; P:lateral root formation; IEA:EnsemblPlants.
DR GO; GO:0007112; P:male meiosis cytokinesis; IEA:EnsemblPlants.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..355
FT /note="Mitogen-activated protein kinase kinase 1"
FT /id="PRO_0000239761"
FT DOMAIN 71..330
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 193
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 77..85
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 100
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 221
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT MOD_RES 227
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 355 AA; 40000 MW; B579E8B7DCB861F4 CRC64;
MRGKKPHKEL KLSVPAQETP VDKFLTASGT FKDGELRLNQ RGLQLISEET ADEPQSTNLK
VEDVQLSMDD LEMIQVIGKG SGGIVQLVRH KWVGTLYALK GIQMNIQEAV RKQIVQELKI
NQATQNAHIV LCHQSFYHNG VIYLVLEYMD RGSLADIIKQ VKTILEPYLA VLCKQVLEGL
LYLHHERHVI HRDIKPSNLL VNRKGEVKIT DFGVSAVLAS SMGQRDTFVG TYNYMAPERI
SGSSYDYKSD IWSLGLVILE CAIGRFPYIP SEGEGWLSFY ELLEAIVDQP PPSAPADQFS
PEFCAFISSC IQKDPAERMS ASELLNHPFI KKFEDKDLDL RILVESLEPP MNISE
