5NT1A_MOUSE
ID 5NT1A_MOUSE Reviewed; 365 AA.
AC A3KFX0; B2RVQ9;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Cytosolic 5'-nucleotidase 1A;
DE Short=cN1A;
DE EC=3.1.3.5;
DE AltName: Full=Cytosolic 5'-nucleotidase IA;
DE Short=cN-IA;
GN Name=Nt5c1a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of
CC deoxyribonucleotides and has a broad substrate specificity. Helps to
CC regulate adenosine levels in heart during ischemia and hypoxia (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +
CC phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Activated by ADP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 5'-nucleotidase type 3 family.
CC {ECO:0000305}.
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DR EMBL; AL606934; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466552; EDL30387.1; -; Genomic_DNA.
DR EMBL; BC147308; AAI47309.1; -; mRNA.
DR EMBL; BC147309; AAI47310.1; -; mRNA.
DR EMBL; BC147730; AAI47731.1; -; mRNA.
DR EMBL; BC147733; AAI47734.1; -; mRNA.
DR CCDS; CCDS38871.1; -.
DR RefSeq; NP_001078971.1; NM_001085502.1.
DR AlphaFoldDB; A3KFX0; -.
DR STRING; 10090.ENSMUSP00000069422; -.
DR iPTMnet; A3KFX0; -.
DR PhosphoSitePlus; A3KFX0; -.
DR MaxQB; A3KFX0; -.
DR PaxDb; A3KFX0; -.
DR PeptideAtlas; A3KFX0; -.
DR PRIDE; A3KFX0; -.
DR ProteomicsDB; 296420; -.
DR Antibodypedia; 54865; 207 antibodies from 25 providers.
DR Ensembl; ENSMUST00000068262; ENSMUSP00000069422; ENSMUSG00000054958.
DR GeneID; 230718; -.
DR KEGG; mmu:230718; -.
DR UCSC; uc008uoy.1; mouse.
DR CTD; 84618; -.
DR MGI; MGI:2155700; Nt5c1a.
DR VEuPathDB; HostDB:ENSMUSG00000054958; -.
DR eggNOG; ENOG502QRJF; Eukaryota.
DR GeneTree; ENSGT00390000017767; -.
DR HOGENOM; CLU_060123_0_0_1; -.
DR InParanoid; A3KFX0; -.
DR OMA; IRTLMAW; -.
DR OrthoDB; 694318at2759; -.
DR PhylomeDB; A3KFX0; -.
DR TreeFam; TF329831; -.
DR Reactome; R-MMU-73621; Pyrimidine catabolism.
DR Reactome; R-MMU-74259; Purine catabolism.
DR BioGRID-ORCS; 230718; 1 hit in 72 CRISPR screens.
DR PRO; PR:A3KFX0; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A3KFX0; protein.
DR Bgee; ENSMUSG00000054958; Expressed in hindlimb stylopod muscle and 30 other tissues.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0008253; F:5'-nucleotidase activity; ISO:MGI.
DR GO; GO:0050483; F:IMP 5'-nucleotidase activity; ISO:MGI.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0106411; F:XMP 5'-nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046085; P:adenosine metabolic process; ISO:MGI.
DR GO; GO:0000255; P:allantoin metabolic process; ISO:MGI.
DR GO; GO:0006196; P:AMP catabolic process; ISO:MGI.
DR GO; GO:0046059; P:dAMP catabolic process; ISO:MGI.
DR GO; GO:0046055; P:dGMP catabolic process; ISO:MGI.
DR GO; GO:0006204; P:IMP catabolic process; ISO:MGI.
DR GO; GO:0009128; P:purine nucleoside monophosphate catabolic process; IMP:MGI.
DR InterPro; IPR010394; 5-nucleotidase.
DR PANTHER; PTHR31367; PTHR31367; 1.
DR Pfam; PF06189; 5-nucleotidase; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Magnesium; Nucleotide metabolism; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..365
FT /note="Cytosolic 5'-nucleotidase 1A"
FT /id="PRO_0000304930"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 208
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 365 AA; 40678 MW; 10DBCBE793C69D64 CRC64;
MEPGQPREAR EPGPGAETAA VPRWEEAKTF YDNLSSKKKP KSPKPQNAVT IAVSSRALFR
MDEEQRIYTE QGVEEYVRYQ LEHENEPFSP GPAFPFVKAL EAVNKRLREL YPDSEDIFDI
VLMTNNHAQV GVRLINSINH YDLFIERFCM TGGNSPICYL KAYHTNLYLS ADADKVREAI
DEGIAAATIF SPSRDVVVSQ SQLRVAFDGD AVLFSDESER IVKAHGLDRF FEHEKAHENK
PLAQGPLKGF LEALGRLQKK FYSKGLRLEC PIRTYLVTAR SAASSGARAL KTLRSWGLET
DEALFLAGAP KGPLLEKIRP HIFFDDQMFH VAGAQEMGTV AAHVPYGVAQ NPRRAAAAKQ
SLGAQ
