ID   M3A_CONAX               Reviewed;          15 AA.
AC   P0DOZ8;
DT   12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2017, sequence version 1.
DT   22-APR-2020, entry version 9.
DE   RecName: Full=Conotoxin Asi3a {ECO:0000303|PubMed:27801785};
OS   Conus asiaticus (Cone snail).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Phasmoconus.
OX   NCBI_TaxID=1945508;
RN   [1]
RP   PROTEIN SEQUENCE, AMIDATION AT TYR-15, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, SYNTHESIS, AND FUNCTION.
RC   TISSUE=Venom;
RX   PubMed=27801785; DOI=10.3390/md14110199;
RA   Lebbe E.K., Ghequire M.G., Peigneur S., Mille B.G., Devi P.,
RA   Ravichandran S., Waelkens E., D'Souza L., De Mot R., Tytgat J.;
RT   "Novel conopeptides of largely unexplored indo Pacific Conus sp.";
RL   Mar. Drugs 14:199-216(2016).
CC   -!- FUNCTION: Does not show any effect on voltage-gated sodium and
CC       potassium channels (10 uM of toxin tested), and on nicotinic
CC       acetylcholine receptors (5 uM of toxin tested) (PubMed:27801785). Does
CC       not show antibacterial activity on both Gram-negative and Gram-positive
CC       bacteria (PubMed:27801785). Intraperitoneal injection into fish
CC       provokes paralysis (By similarity). {ECO:0000250|UniProtKB:P0CH15,
CC       ECO:0000269|PubMed:27801785}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27801785}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000269|PubMed:27801785}.
CC   -!- DOMAIN: The cysteine framework is III (CC-C-C-CC). Classified in the M-
CC       2 branch, since 2 residues stand between the fourth and the fifth
CC       cysteine residues. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1697.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27801785};
CC   -!- MISCELLANEOUS: Does not show effect on Nav1.1, Nav1.2, Nav1.3, Nav1.4,
CC       Nav1.5, Nav1.6, Nav1.8 (PubMed:27801785). Does not show effect on
CC       Kv1.1, Kv1.2, Kv1.3, Kv1.4, Kv1.5, and Kv1.6 (PubMed:27801785). Does
CC       not show effect on muscular (alpha-1-beta-1-delta-epsilon) and neuronal
CC       alpha-7, alpha-4-beta-4, and alpha-3-beta-4 nAChRs (PubMed:27801785).
CC       {ECO:0000269|PubMed:27801785}.
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..15
FT                   /note="Conotoxin Asi3a"
FT                   /evidence="ECO:0000269|PubMed:27801785"
FT                   /id="PRO_0000439517"
FT   MOD_RES         15
FT                   /note="Tyrosine amide"
FT                   /evidence="ECO:0000269|PubMed:27801785"
FT   DISULFID        1..14
FT                   /evidence="ECO:0000250|UniProtKB:P0CI24"
FT   DISULFID        2..10
FT                   /evidence="ECO:0000250|UniProtKB:P0CI24"
FT   DISULFID        6..13
FT                   /evidence="ECO:0000250|UniProtKB:P0CI24"
SQ   SEQUENCE   15 AA;  1703 MW;  E5D374F4EF5965FE CRC64;
     CCQWPCSHGC IPCCY