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M3K10_HUMAN
ID   M3K10_HUMAN             Reviewed;         954 AA.
AC   Q02779; Q12761; Q14871;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 3.
DT   03-AUG-2022, entry version 202.
DE   RecName: Full=Mitogen-activated protein kinase kinase kinase 10;
DE            EC=2.7.11.25;
DE   AltName: Full=Mixed lineage kinase 2;
DE   AltName: Full=Protein kinase MST;
GN   Name=MAP3K10; Synonyms=MLK2, MST;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=8536694; DOI=10.1111/j.1432-1033.1995.492_b.x;
RA   Dorow D.S., Devereux L., Tu G.F., Price G., Nicholl J.K., Sutherland G.R.,
RA   Simpson R.J.;
RT   "Complete nucleotide sequence, expression, and chromosomal localisation of
RT   human mixed-lineage kinase 2.";
RL   Eur. J. Biochem. 234:492-500(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=7731697;
RA   Katoh M., Hirai M., Sugimura T., Terada M.;
RT   "Cloning and characterization of MST, a novel (putative) serine/threonine
RT   kinase with SH3 domain.";
RL   Oncogene 10:1447-1451(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 244-480.
RC   TISSUE=Colon epithelium;
RX   PubMed=8477742; DOI=10.1111/j.1432-1033.1993.tb17810.x;
RA   Dorow D.S., Devereux L., Dietzsch E., de Kretser T.;
RT   "Identification of a new family of human epithelial protein kinases
RT   containing two leucine/isoleucine-zipper domains.";
RL   Eur. J. Biochem. 213:701-710(1993).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498; SER-502 AND THR-558, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA   Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA   Mann M., Daub H.;
RT   "Large-scale proteomics analysis of the human kinome.";
RL   Mol. Cell. Proteomics 8:1751-1764(2009).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [6]
RP   VARIANT [LARGE SCALE ANALYSIS] GLU-107.
RX   PubMed=17344846; DOI=10.1038/nature05610;
RA   Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G.,
RA   Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S.,
RA   Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.,
RA   Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K.,
RA   Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D.,
RA   Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R.,
RA   Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A.,
RA   Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F.,
RA   Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F.,
RA   Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G.,
RA   Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R.,
RA   Futreal P.A., Stratton M.R.;
RT   "Patterns of somatic mutation in human cancer genomes.";
RL   Nature 446:153-158(2007).
CC   -!- FUNCTION: Activates the JUN N-terminal pathway. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.25;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: Homodimerization via the leucine zipper domains is
CC       required for autophosphorylation and subsequent activation.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with SH3RF2. {ECO:0000250,
CC       ECO:0000250|UniProtKB:D3ZG83}.
CC   -!- INTERACTION:
CC       Q02779; Q5TCX8: MAP3K21; NbExp=3; IntAct=EBI-3392815, EBI-1057380;
CC   -!- TISSUE SPECIFICITY: Expressed in brain and skeletal muscle.
CC   -!- PTM: Autophosphorylation on serine and threonine residues within the
CC       activation loop plays a role in enzyme activation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC       protein kinase family. MAP kinase kinase kinase subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X90846; CAA62351.1; -; mRNA.
DR   EMBL; Z48615; CAA88531.1; -; mRNA.
DR   CCDS; CCDS12549.1; -.
DR   PIR; S68178; S68178.
DR   RefSeq; NP_002437.2; NM_002446.3.
DR   PDB; 2RF0; X-ray; 2.00 A; A/B/C/D=13-78.
DR   PDBsum; 2RF0; -.
DR   AlphaFoldDB; Q02779; -.
DR   SMR; Q02779; -.
DR   BioGRID; 110440; 40.
DR   ELM; Q02779; -.
DR   IntAct; Q02779; 15.
DR   STRING; 9606.ENSP00000253055; -.
DR   BindingDB; Q02779; -.
DR   ChEMBL; CHEMBL2873; -.
DR   DrugBank; DB12010; Fostamatinib.
DR   DrugCentral; Q02779; -.
DR   GuidetoPHARMACOLOGY; 2070; -.
DR   iPTMnet; Q02779; -.
DR   PhosphoSitePlus; Q02779; -.
DR   BioMuta; MAP3K10; -.
DR   DMDM; 145559494; -.
DR   jPOST; Q02779; -.
DR   MassIVE; Q02779; -.
DR   MaxQB; Q02779; -.
DR   PaxDb; Q02779; -.
DR   PeptideAtlas; Q02779; -.
DR   PRIDE; Q02779; -.
DR   ProteomicsDB; 58124; -.
DR   TopDownProteomics; Q02779; -.
DR   Antibodypedia; 2053; 266 antibodies from 33 providers.
DR   DNASU; 4294; -.
DR   Ensembl; ENST00000253055.8; ENSP00000253055.2; ENSG00000130758.8.
DR   GeneID; 4294; -.
DR   KEGG; hsa:4294; -.
DR   MANE-Select; ENST00000253055.8; ENSP00000253055.2; NM_002446.4; NP_002437.2.
DR   UCSC; uc002ona.3; human.
DR   CTD; 4294; -.
DR   DisGeNET; 4294; -.
DR   GeneCards; MAP3K10; -.
DR   HGNC; HGNC:6849; MAP3K10.
DR   HPA; ENSG00000130758; Tissue enhanced (brain).
DR   MIM; 600137; gene.
DR   neXtProt; NX_Q02779; -.
DR   OpenTargets; ENSG00000130758; -.
DR   PharmGKB; PA30593; -.
DR   VEuPathDB; HostDB:ENSG00000130758; -.
DR   eggNOG; KOG0192; Eukaryota.
DR   GeneTree; ENSGT00940000160518; -.
DR   HOGENOM; CLU_000288_7_14_1; -.
DR   InParanoid; Q02779; -.
DR   OMA; CNQRKKS; -.
DR   OrthoDB; 115270at2759; -.
DR   PhylomeDB; Q02779; -.
DR   TreeFam; TF105118; -.
DR   PathwayCommons; Q02779; -.
DR   SABIO-RK; Q02779; -.
DR   SignaLink; Q02779; -.
DR   SIGNOR; Q02779; -.
DR   BioGRID-ORCS; 4294; 10 hits in 1114 CRISPR screens.
DR   ChiTaRS; MAP3K10; human.
DR   EvolutionaryTrace; Q02779; -.
DR   GeneWiki; MAP3K10; -.
DR   GenomeRNAi; 4294; -.
DR   Pharos; Q02779; Tchem.
DR   PRO; PR:Q02779; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q02779; protein.
DR   Bgee; ENSG00000130758; Expressed in right frontal lobe and 125 other tissues.
DR   ExpressionAtlas; Q02779; baseline and differential.
DR   Genevisible; Q02779; HS.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043425; F:bHLH transcription factor binding; IPI:UniProtKB.
DR   GO; GO:0004706; F:JUN kinase kinase kinase activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0004672; F:protein kinase activity; TAS:ProtInc.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
DR   GO; GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
DR   GO; GO:0006915; P:apoptotic process; TAS:ProtInc.
DR   GO; GO:0007254; P:JNK cascade; TAS:ProtInc.
DR   GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB.
DR   GO; GO:0018107; P:peptidyl-threonine phosphorylation; IDA:UniProtKB.
DR   GO; GO:0043065; P:positive regulation of apoptotic process; IBA:GO_Central.
DR   GO; GO:0046330; P:positive regulation of JNK cascade; IMP:UniProtKB.
DR   GO; GO:0043507; P:positive regulation of JUN kinase activity; IMP:UniProtKB.
DR   GO; GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR   GO; GO:0007224; P:smoothened signaling pathway; IMP:UniProtKB.
DR   CDD; cd12059; SH3_MLK1-3; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR015785; MAP3K10.
DR   InterPro; IPR035779; MLK1-3_SH3.
DR   InterPro; IPR016231; MLK1-4.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR44329:SF39; PTHR44329:SF39; 1.
DR   Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR   Pfam; PF14604; SH3_9; 1.
DR   PIRSF; PIRSF000556; MAPKKK9_11; 1.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   PRINTS; PR00109; TYRKINASE.
DR   SMART; SM00220; S_TKc; 1.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF50044; SSF50044; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR   PROSITE; PS50002; SH3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Kinase; Methylation; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Repeat;
KW   Serine/threonine-protein kinase; SH3 domain; Transferase.
FT   CHAIN           1..954
FT                   /note="Mitogen-activated protein kinase kinase kinase 10"
FT                   /id="PRO_0000086259"
FT   DOMAIN          16..81
FT                   /note="SH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          98..360
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          384..405
FT                   /note="Leucine-zipper 1"
FT   REGION          419..440
FT                   /note="Leucine-zipper 2"
FT   REGION          490..665
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          716..739
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          757..954
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        523..538
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        644..658
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        771..790
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        933..948
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        222
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         104..112
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         125
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   MOD_RES         258
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         262
FT                   /note="Phosphoserine; by autocatalysis and MAP4K1"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         498
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19369195"
FT   MOD_RES         502
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19369195"
FT   MOD_RES         506
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19690332"
FT   MOD_RES         558
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:19369195"
FT   MOD_RES         857
FT                   /note="Omega-N-methylarginine"
FT                   /evidence="ECO:0000250|UniProtKB:Q66L42"
FT   VARIANT         107
FT                   /note="G -> E (in a metastatic melanoma sample; somatic
FT                   mutation)"
FT                   /evidence="ECO:0000269|PubMed:17344846"
FT                   /id="VAR_040702"
FT   VARIANT         168
FT                   /note="P -> Q (in dbSNP:rs36102209)"
FT                   /id="VAR_051639"
FT   CONFLICT        462..464
FT                   /note="SRL -> AV (in Ref. 2; CAA88531)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        465..480
FT                   /note="LKLREGGSHISLPSGF -> AQAAGRRQPHQPALWL (in Ref. 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        471
FT                   /note="G -> S (in Ref. 2; CAA88531)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        807
FT                   /note="R -> G (in Ref. 1; CAA62351)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        818
FT                   /note="A -> V (in Ref. 1; CAA62351)"
FT                   /evidence="ECO:0000305"
FT   STRAND          20..23
FT                   /evidence="ECO:0007829|PDB:2RF0"
FT   STRAND          42..47
FT                   /evidence="ECO:0007829|PDB:2RF0"
FT   HELIX           50..53
FT                   /evidence="ECO:0007829|PDB:2RF0"
FT   STRAND          58..62
FT                   /evidence="ECO:0007829|PDB:2RF0"
FT   STRAND          68..72
FT                   /evidence="ECO:0007829|PDB:2RF0"
FT   HELIX           73..75
FT                   /evidence="ECO:0007829|PDB:2RF0"
SQ   SEQUENCE   954 AA;  103694 MW;  59A7596B05751981 CRC64;
     MEEEEGAVAK EWGTTPAGPV WTAVFDYEAA GDEELTLRRG DRVQVLSQDC AVSGDEGWWT
     GQLPSGRVGV FPSNYVAPGA PAAPAGLQLP QEIPFHELQL EEIIGVGGFG KVYRALWRGE
     EVAVKAARLD PEKDPAVTAE QVCQEARLFG ALQHPNIIAL RGACLNPPHL CLVMEYARGG
     ALSRVLAGRR VPPHVLVNWA VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA
     DTVLKITDFG LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE
     VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF GSILKRLEVI
     EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS REEELLRAAQ EQRFQEEQLR
     RREQELAERE MDIVERELHL LMCQLSQEKP RVRKRKGNFK RSRLLKLREG GSHISLPSGF
     EHKITVQASP TLDKRKGSDG ASPPASPSII PRLRAIRLTP VDCGGSSSGS SSGGSGTWSR
     GGPPKKEELV GGKKKGRTWG PSSTLQKERV GGEERLKGLG EGSKQWSSSA PNLGKSPKHT
     PIAPGFASLN EMEEFAEAED GGSSVPPSPY STPSYLSVPL PAEPSPGARA PWEPTPSAPP
     ARWGHGARRR CDLALLGCAT LLGAVGLGAD VAEARAADGE EQRRWLDGLF FPRAGRFPRG
     LSPPARPHGR REDVGPGLGL APSATLVSLS SVSDCNSTRS LLRSDSDEAA PAAPSPPPSP
     PAPTPTPSPS TNPLVDLELE SFKKDPRQSL TPTHVTAACA VSRGHRRTPS DGALGQRGPP
     EPAGHGPGPR DLLDFPRLPD PQALFPARRR PPEFPGRPTT LTFAPRPRPA ASRPRLDPWK
     LVSFGRTLTI SPPSRPDTPE SPGPPSVQPT LLDMDMEGQN QDSTVPLCGA HGSH
 
 
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