5NT1B_HUMAN
ID 5NT1B_HUMAN Reviewed; 610 AA.
AC Q96P26; B5MCR0; B7ZVX7; Q53RX2; Q8N9W3; Q8NA26; Q96DU5; Q96KE6; Q96M25;
AC Q96SA3;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cytosolic 5'-nucleotidase 1B;
DE Short=cN1B;
DE EC=3.1.3.5;
DE AltName: Full=Autoimmune infertility-related protein;
DE AltName: Full=Cytosolic 5'-nucleotidase IB;
DE Short=cN-IB;
GN Name=NT5C1B; Synonyms=AIRP; ORFNames=FKSG85;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RA Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S.;
RT "Isolation of a novel human testis cDNA encoding a polypeptide related to
RT autoimmune infertility.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Ji Y., Mitchell B.S., Spychala J.;
RT "Homo sapiens cytosolic nucleotidase Ib alpha mRNA.";
RL Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 312-610.
RA Wang Y.-G., Gong L.;
RT "Identification and characterization of FKSG85 which encodes a nucleotidase
RT protein.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 325-601, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=11690631; DOI=10.1016/s0167-4781(01)00278-0;
RA Sala-Newby G.B., Newby A.C.;
RT "Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene
RT related to human autoimmune infertility-related protein.";
RL Biochim. Biophys. Acta 1521:12-18(2001).
CC -!- FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of
CC deoxyribonucleotides. Helps to regulate adenosine levels (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +
CC phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Activated by ADP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=CN-Ib alpha;
CC IsoId=Q96P26-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96P26-2; Sequence=VSP_010201;
CC Name=3;
CC IsoId=Q96P26-3; Sequence=VSP_010202;
CC Name=4;
CC IsoId=Q96P26-4; Sequence=VSP_010203;
CC -!- TISSUE SPECIFICITY: Highly expressed in testis, placenta and pancreas.
CC Detected at lower levels in heart, kidney, liver and lung.
CC {ECO:0000269|PubMed:11690631}.
CC -!- SIMILARITY: Belongs to the 5'-nucleotidase type 3 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK39108.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK83285.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAC04103.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAC44363.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF356185; AAK39108.1; ALT_INIT; mRNA.
DR EMBL; AF417165; AAL11910.1; -; mRNA.
DR EMBL; AK093234; BAC04103.1; ALT_INIT; mRNA.
DR EMBL; AK093464; BAC04174.1; -; mRNA.
DR EMBL; AK057444; BAB71489.1; -; mRNA.
DR EMBL; AC079148; AAX93260.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00853.1; -; Genomic_DNA.
DR EMBL; BC136387; AAI36388.1; -; mRNA.
DR EMBL; BC171761; AAI71761.1; -; mRNA.
DR EMBL; AF352326; AAK83285.1; ALT_FRAME; mRNA.
DR EMBL; AJ295254; CAC44363.1; ALT_INIT; mRNA.
DR CCDS; CCDS33149.1; -. [Q96P26-2]
DR CCDS; CCDS33150.1; -. [Q96P26-1]
DR RefSeq; NP_001002006.1; NM_001002006.2. [Q96P26-1]
DR RefSeq; NP_001186015.1; NM_001199086.1.
DR RefSeq; NP_001186016.1; NM_001199087.1.
DR RefSeq; NP_001186017.1; NM_001199088.1.
DR RefSeq; NP_001186033.1; NM_001199104.1. [Q96P26-4]
DR RefSeq; NP_150278.2; NM_033253.3. [Q96P26-2]
DR AlphaFoldDB; Q96P26; -.
DR BioGRID; 124995; 3.
DR STRING; 9606.ENSP00000352904; -.
DR DEPOD; NT5C1B; -.
DR iPTMnet; Q96P26; -.
DR PhosphoSitePlus; Q96P26; -.
DR BioMuta; NT5C1B; -.
DR DMDM; 47116569; -.
DR MassIVE; Q96P26; -.
DR PaxDb; Q96P26; -.
DR PeptideAtlas; Q96P26; -.
DR PRIDE; Q96P26; -.
DR ProteomicsDB; 77604; -. [Q96P26-1]
DR ProteomicsDB; 77605; -. [Q96P26-2]
DR ProteomicsDB; 77606; -. [Q96P26-3]
DR ProteomicsDB; 77607; -. [Q96P26-4]
DR TopDownProteomics; Q96P26-3; -. [Q96P26-3]
DR Antibodypedia; 47312; 115 antibodies from 21 providers.
DR DNASU; 100526794; -.
DR Ensembl; ENST00000304081.8; ENSP00000305979.4; ENSG00000185013.16. [Q96P26-2]
DR Ensembl; ENST00000359846.6; ENSP00000352904.2; ENSG00000185013.16. [Q96P26-1]
DR GeneID; 100526794; -.
DR GeneID; 93034; -.
DR KEGG; hsa:100526794; -.
DR KEGG; hsa:93034; -.
DR MANE-Select; ENST00000304081.9; ENSP00000305979.4; NM_033253.4; NP_150278.2. [Q96P26-2]
DR UCSC; uc002rcz.4; human. [Q96P26-1]
DR CTD; 100526794; -.
DR CTD; 93034; -.
DR DisGeNET; 100526794; -.
DR DisGeNET; 93034; -.
DR GeneCards; NT5C1B; -.
DR HGNC; HGNC:17818; NT5C1B.
DR HPA; ENSG00000185013; Tissue enriched (testis).
DR MIM; 610526; gene.
DR neXtProt; NX_Q96P26; -.
DR OpenTargets; ENSG00000185013; -.
DR PharmGKB; PA31800; -.
DR VEuPathDB; HostDB:ENSG00000185013; -.
DR eggNOG; ENOG502QRJF; Eukaryota.
DR GeneTree; ENSGT00390000017767; -.
DR HOGENOM; CLU_035579_0_0_1; -.
DR OMA; YIPAFNA; -.
DR OrthoDB; 694318at2759; -.
DR PhylomeDB; Q96P26; -.
DR TreeFam; TF329831; -.
DR PathwayCommons; Q96P26; -.
DR Reactome; R-HSA-74259; Purine catabolism.
DR SABIO-RK; Q96P26; -.
DR BioGRID-ORCS; 100526794; 14 hits in 591 CRISPR screens.
DR BioGRID-ORCS; 93034; 7 hits in 1002 CRISPR screens.
DR Pharos; Q96P26; Tbio.
DR PRO; PR:Q96P26; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q96P26; protein.
DR Bgee; ENSG00000185013; Expressed in sperm and 99 other tissues.
DR ExpressionAtlas; Q96P26; baseline and differential.
DR Genevisible; Q96P26; HS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0106411; F:XMP 5'-nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046085; P:adenosine metabolic process; IBA:GO_Central.
DR GO; GO:0006195; P:purine nucleotide catabolic process; TAS:Reactome.
DR InterPro; IPR010394; 5-nucleotidase.
DR PANTHER; PTHR31367; PTHR31367; 1.
DR Pfam; PF06189; 5-nucleotidase; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Hydrolase; Magnesium;
KW Nucleotide metabolism; Reference proteome.
FT CHAIN 1..610
FT /note="Cytosolic 5'-nucleotidase 1B"
FT /id="PRO_0000144796"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..171
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..201
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 467
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..208
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_010202"
FT VAR_SEQ 41..100
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_010201"
FT VAR_SEQ 596..610
FT /note="LGSIAAYGFNKKFSS -> KSLGWMS (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_010203"
FT CONFLICT 173
FT /note="G -> R (in Ref. 2; AAL11910)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="R -> K (in Ref. 1; AAK39108)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="S -> W (in Ref. 1; AAK39108)"
FT /evidence="ECO:0000305"
FT CONFLICT 351
FT /note="P -> Q (in Ref. 2; AAL11910)"
FT /evidence="ECO:0000305"
FT CONFLICT 362
FT /note="V -> I (in Ref. 3; BAC04174)"
FT /evidence="ECO:0000305"
FT CONFLICT 528
FT /note="L -> P (in Ref. 8; CAC44363)"
FT /evidence="ECO:0000305"
FT CONFLICT 552
FT /note="L -> F (in Ref. 1; AAK39108)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 610 AA; 68804 MW; EEC0BE9E5498B3E9 CRC64;
MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS LRCCPFQGHS
SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR GSQESSLRKT DSRGYLVRSQ
WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP
ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP
TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW
PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ
YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK
AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF
TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS
AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA
AYGFNKKFSS
