M4A8_MOUSE
ID M4A8_MOUSE Reviewed; 290 AA.
AC Q99N10; F8WIR2; Q2TVW6; Q3T990; Q8CG94; Q9EQZ0;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 4.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Membrane-spanning 4-domains subfamily A member 8;
DE AltName: Full=CD20 antigen-like 5;
DE AltName: Full=Membrane-spanning 4-domains subfamily A member 8A;
GN Name=Ms4a8; Synonyms=Cd20l5, Ms4a8a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=DBA/2J, and FVB/N; TISSUE=Colon;
RX PubMed=11401424; DOI=10.1006/geno.2000.6472;
RA Liang Y., Tedder T.F.;
RT "Identification of a CD20-, Fc-epsilon-RI-beta-, and HTm4-related gene
RT family: sixteen new MS4A family members expressed in human and mouse.";
RL Genomics 72:119-127(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=11245982; DOI=10.1016/s0378-1119(00)00598-9;
RA Ishibashi K., Suzuki M., Sasaki S., Imai M.;
RT "Identification of a new multigene four-transmembrane family (MS4A) related
RT to CD20, HTm4 and beta subunit of the high-affinity IgE receptor.";
RL Gene 264:87-93(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-152 (ISOFORM 1).
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-290 (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in signal transduction as a component of a
CC multimeric receptor complex.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99N10-3; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99N10-1; Sequence=VSP_053843;
CC -!- TISSUE SPECIFICITY: Expressed strongly in intestine and colon and
CC minimally in lung and ovary. {ECO:0000269|PubMed:11245982}.
CC -!- MISCELLANEOUS: [Isoform 2]: Not found in strain C57BL/6J but has been
CC identified in strains DBA/2J and FVB/N. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MS4A family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE43134.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AF237906; AAK37415.1; -; mRNA.
DR EMBL; AY039038; AAK92698.1; -; mRNA.
DR EMBL; AB026044; BAB18756.1; -; mRNA.
DR EMBL; AC148321; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK172700; BAE43134.1; ALT_SEQ; mRNA.
DR EMBL; BC042719; AAH42719.1; -; mRNA.
DR CCDS; CCDS37921.1; -. [Q99N10-3]
DR RefSeq; NP_071875.2; NM_022430.2. [Q99N10-3]
DR AlphaFoldDB; Q99N10; -.
DR SMR; Q99N10; -.
DR STRING; 10090.ENSMUSP00000025636; -.
DR PhosphoSitePlus; Q99N10; -.
DR PaxDb; Q99N10; -.
DR PRIDE; Q99N10; -.
DR Antibodypedia; 1542; 144 antibodies from 17 providers.
DR DNASU; 64381; -.
DR Ensembl; ENSMUST00000025636; ENSMUSP00000025636; ENSMUSG00000024730. [Q99N10-3]
DR GeneID; 64381; -.
DR KEGG; mmu:64381; -.
DR UCSC; uc008gro.1; mouse. [Q99N10-3]
DR CTD; 64381; -.
DR MGI; MGI:1927657; Ms4a8a.
DR VEuPathDB; HostDB:ENSMUSG00000024730; -.
DR eggNOG; ENOG502SR7E; Eukaryota.
DR GeneTree; ENSGT00940000162329; -.
DR HOGENOM; CLU_091032_1_0_1; -.
DR InParanoid; Q99N10; -.
DR OMA; QLVCCQH; -.
DR OrthoDB; 1256119at2759; -.
DR PhylomeDB; Q99N10; -.
DR TreeFam; TF335157; -.
DR BioGRID-ORCS; 64381; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Ms4a8a; mouse.
DR PRO; PR:Q99N10; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q99N10; protein.
DR Bgee; ENSMUSG00000024730; Expressed in left colon and 57 other tissues.
DR Genevisible; Q99N10; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR007237; CD20-like.
DR InterPro; IPR030417; MS4A.
DR InterPro; IPR030424; MS4A8.
DR PANTHER; PTHR23320; PTHR23320; 1.
DR PANTHER; PTHR23320:SF128; PTHR23320:SF128; 1.
DR Pfam; PF04103; CD20; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Receptor; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..290
FT /note="Membrane-spanning 4-domains subfamily A member 8"
FT /id="PRO_0000158644"
FT TOPO_DOM 1..108
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..138
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..220
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..23
FT /note="MNRPTAQGAVNLSGSKFSTAKSW -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11245982,
FT ECO:0000303|PubMed:11401424"
FT /id="VSP_053843"
FT CONFLICT 32
FT /note="W -> R (in Ref. 2; AAK92698)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="V -> G (in Ref. 2; AAK92698)"
FT /evidence="ECO:0000305"
FT CONFLICT 140
FT /note="S -> Y (in Ref. 2; AAK92698)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="F -> V (in Ref. 2; AAK92698)"
FT /evidence="ECO:0000305"
FT CONFLICT 163
FT /note="A -> G (in Ref. 2; AAK92698)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 30858 MW; F4373EB2CE53AA75 CRC64;
MNRPTAQGAV NLSGSKFSTA KSWEPEQERL TWQPGTVSMN TVTSPGPMAN SVYVVAPPNS
YPVVPGTVPQ MPIYPSNQPQ VHVISGHLPG LVPAMTEPPA QRVLKKGQVL GAIQILIGLV
HIGLGSIMIT NLFSHYTPVS LYGGFPFWGG IWFIISGSLS VAAETQPNSP CLLNGSVGLN
IFSAICSAVG IMLFITDISI SSGYIYPSYY PYQENLGVRT GVAISSVLLI FCLLELSIAS
VSSHFGCQVA CCHYNNPGVV IPNVYAANPV VIPEPPNPIP SYSEVVQDSR
