5NT1B_MOUSE
ID 5NT1B_MOUSE Reviewed; 573 AA.
AC Q91YE9; E9QNH2; Q91Y48;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Cytosolic 5'-nucleotidase 1B;
DE Short=cN1B;
DE EC=3.1.3.5 {ECO:0000305|PubMed:11690631};
DE AltName: Full=Autoimmune infertility-related protein;
DE AltName: Full=Cytosolic 5'-nucleotidase IB;
DE Short=cN-IB;
GN Name=Nt5c1b; Synonyms=Airp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=11690631; DOI=10.1016/s0167-4781(01)00278-0;
RA Sala-Newby G.B., Newby A.C.;
RT "Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene
RT related to human autoimmune infertility-related protein.";
RL Biochim. Biophys. Acta 1521:12-18(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=CD-1; TISSUE=Testis;
RA Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S.;
RT "Isolation of a novel human testis cDNA encoding a polypeptide related to
RT autoimmune infertility.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of
CC deoxyribonucleotides. Helps to regulate adenosine levels.
CC {ECO:0000269|PubMed:11690631}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside +
CC phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5;
CC Evidence={ECO:0000305|PubMed:11690631};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC -!- ACTIVITY REGULATION: Activated by ADP.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91YE9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91YE9-2; Sequence=VSP_010204, VSP_010205;
CC -!- TISSUE SPECIFICITY: Expressed at highest levels in testis. Also
CC expressed in brain, skeletal muscle, kidney and heart.
CC {ECO:0000269|PubMed:11690631}.
CC -!- SIMILARITY: Belongs to the 5'-nucleotidase type 3 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK39109.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ295253; CAC44364.1; -; mRNA.
DR EMBL; AF356186; AAK39109.1; ALT_INIT; mRNA.
DR EMBL; AC157570; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS25810.1; -. [Q91YE9-1]
DR CCDS; CCDS79106.1; -. [Q91YE9-2]
DR RefSeq; NP_081864.2; NM_027588.3. [Q91YE9-1]
DR AlphaFoldDB; Q91YE9; -.
DR STRING; 10090.ENSMUSP00000002456; -.
DR iPTMnet; Q91YE9; -.
DR PhosphoSitePlus; Q91YE9; -.
DR PaxDb; Q91YE9; -.
DR PRIDE; Q91YE9; -.
DR ProteomicsDB; 296421; -. [Q91YE9-1]
DR ProteomicsDB; 296422; -. [Q91YE9-2]
DR Ensembl; ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622. [Q91YE9-1]
DR Ensembl; ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622. [Q91YE9-2]
DR GeneID; 70881; -.
DR KEGG; mmu:70881; -.
DR UCSC; uc007nap.2; mouse. [Q91YE9-1]
DR CTD; 93034; -.
DR MGI; MGI:1918131; Nt5c1b.
DR VEuPathDB; HostDB:ENSMUSG00000020622; -.
DR eggNOG; ENOG502QRJF; Eukaryota.
DR GeneTree; ENSGT00390000017767; -.
DR HOGENOM; CLU_035579_0_0_1; -.
DR InParanoid; Q91YE9; -.
DR OMA; EDEAYWA; -.
DR PhylomeDB; Q91YE9; -.
DR TreeFam; TF329831; -.
DR Reactome; R-MMU-74259; Purine catabolism.
DR SABIO-RK; Q91YE9; -.
DR BioGRID-ORCS; 70881; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Nt5c1b; mouse.
DR PRO; PR:Q91YE9; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q91YE9; protein.
DR Bgee; ENSMUSG00000020622; Expressed in seminiferous tubule of testis and 12 other tissues.
DR ExpressionAtlas; Q91YE9; baseline and differential.
DR Genevisible; Q91YE9; MM.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0008253; F:5'-nucleotidase activity; IDA:MGI.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0106411; F:XMP 5'-nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046085; P:adenosine metabolic process; ISO:MGI.
DR GO; GO:0000255; P:allantoin metabolic process; IDA:MGI.
DR GO; GO:0006196; P:AMP catabolic process; IDA:MGI.
DR GO; GO:0009116; P:nucleoside metabolic process; IDA:MGI.
DR InterPro; IPR010394; 5-nucleotidase.
DR PANTHER; PTHR31367; PTHR31367; 1.
DR Pfam; PF06189; 5-nucleotidase; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Hydrolase; Magnesium;
KW Nucleotide metabolism; Reference proteome.
FT CHAIN 1..573
FT /note="Cytosolic 5'-nucleotidase 1B"
FT /id="PRO_0000144797"
FT REGION 1..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 218..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 428
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT VAR_SEQ 88..89
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_010204"
FT VAR_SEQ 247..262
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_010205"
FT CONFLICT 103
FT /note="S -> F (in Ref. 2; AAK39109)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="I -> T (in Ref. 1; CAC44364 and 2; AAK39109)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="A -> V (in Ref. 1; CAC44364)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="E -> Q (in Ref. 1; CAC44364)"
FT /evidence="ECO:0000305"
FT CONFLICT 228
FT /note="Q -> H (in Ref. 1; CAC44364)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 573 AA; 65002 MW; 948868F505D6B965 CRC64;
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN TDSRGYVVRN
QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS SASPSQHETS PPPQTSEKSS
IQQTPQNRPI TQLESQPPTP PETEPNSRRT SAKMYTGSDP WAHRENREPR DLQLRDYAYS
CDSREGMPKT REYPRTPPTE WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD
EAYWTSVSML YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ
LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV GVRLINSVNH
YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI KEGIASATMY AGAKDMAYCD
TQLRVAFDGD AVLFSDESEH IAKDHGLDKF FQHETLFENK PLAQGPLKSF LEDLGKLQKK
FYAKDERLLC PIRTYLVTAR SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP
HIFFDDQMFH IESAQKFGTI TAHVPYGIAQ KRN
