ID   M7E_CONCN               Reviewed;          71 AA.
AC   P0DKQ3; S6CQC0;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2021, sequence version 2.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Omega-conotoxin-like CnVIIE {ECO:0000303|PubMed:22705119};
DE   Contains:
DE     RecName: Full=Omega-conotoxin-like CnVIIC {ECO:0000303|PubMed:22705119};
DE     AltName: Full=[Hyp7-CnVIIC];
DE   Flags: Precursor;
OS   Conus consors (Singed cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Pionoconus.
OX   NCBI_TaxID=101297;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], HYDROXYLATION AT PRO-52, AMIDATION AT CYS-70,
RP   MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR
RP   LOCATION.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=22705119; DOI=10.1016/j.jprot.2012.06.001;
RA   Violette A., Biass D., Dutertre S., Koua D., Piquemal D., Pierrat F.,
RA   Stocklin R., Favreau P.;
RT   "Large-scale discovery of conopeptides and conoproteins in the injectable
RT   venom of a fish-hunting cone snail using a combined proteomic and
RT   transcriptomic approach.";
RL   J. Proteomics 75:5215-5225(2012).
CC   -!- FUNCTION: Omega-conotoxins act at presynaptic membranes, they bind and
CC       block voltage-gated calcium channels (Cav). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22705119}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:22705119}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P05484}.
CC   -!- DOMAIN: The cysteine framework is VI/VII (C-C-CC-C-C). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [Omega-conotoxin-like CnVIIE]: Mass=2649.06;
CC       Method=Electrospray; Note=CnVIIE.;
CC       Evidence={ECO:0000269|PubMed:22705119};
CC   -!- MASS SPECTROMETRY: [Omega-conotoxin-like CnVIIC]: Mass=2575.052;
CC       Method=Electrospray; Note=CnVIIC.;
CC       Evidence={ECO:0000269|PubMed:22705119};
CC   -!- MASS SPECTROMETRY: [Omega-conotoxin-like CnVIIC]: Mass=2591.03;
CC       Method=Electrospray; Note=[Hyp7]-CnVIIC.;
CC       Evidence={ECO:0000269|PubMed:22705119};
CC   -!- MISCELLANEOUS: Found in injectable (milked) (IV) venom.
CC       {ECO:0000305|PubMed:22705119}.
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. {ECO:0000305}.
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DR   EMBL; HE856385; CCI55498.1; -; mRNA.
DR   AlphaFoldDB; P0DKQ3; -.
DR   SMR; P0DKQ3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   InterPro; IPR012321; Conotoxin_omega-typ_CS.
DR   Pfam; PF02950; Conotoxin; 1.
DR   PROSITE; PS60004; OMEGA_CONOTOXIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Calcium channel impairing toxin; Disulfide bond; Hydroxylation;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..45
FT                   /evidence="ECO:0000305|PubMed:22705119"
FT                   /id="PRO_0000451774"
FT   PEPTIDE         46..71
FT                   /note="Omega-conotoxin-like CnVIIE"
FT                   /evidence="ECO:0000269|PubMed:22705119"
FT                   /id="PRO_0000419883"
FT   PEPTIDE         46..70
FT                   /note="Omega-conotoxin-like CnVIIC"
FT                   /evidence="ECO:0000269|PubMed:22705119"
FT                   /id="PRO_0000419884"
FT   MOD_RES         52
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:22705119"
FT   MOD_RES         70
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000269|PubMed:22705119"
FT   DISULFID        46..61
FT                   /evidence="ECO:0000250|UniProtKB:P05484"
FT   DISULFID        53..65
FT                   /evidence="ECO:0000250|UniProtKB:P05484"
FT   DISULFID        60..70
FT                   /evidence="ECO:0000250|UniProtKB:P05484"
SQ   SEQUENCE   71 AA;  7569 MW;  3B133D8567DD1BC0 CRC64;
     MKLTCVVIVA VLLLTACQLI TADDSRGTQK HRALRSDTKL SMSTRCKGTG KPCSRIAYNC
     CTGSCRSGKC G