ID   MA165_PINFU             Reviewed;         129 AA.
AC   O97048;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=N16.5 matrix protein;
DE   AltName: Full=N14#5;
DE   AltName: Full=N14#7;
DE   AltName: Full=Pearlin;
DE   Flags: Precursor;
OS   Pinctada fucata (Akoya pearl oyster) (Pinctada imbricata fucata).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Pterioida; Pterioidea; Pteriidae; Pinctada.
OX   NCBI_TaxID=50426;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mantle;
RX   PubMed=11246407; DOI=10.1007/pl00021687;
RA   Miyashita T., Takagi R., Okushima M., Nakano S., Miyamoto H., Nishikawa E.,
RA   Matsushiro A.;
RT   "Complementary DNA cloning and characterization of pearlin, a new class of
RT   matrix protein in the nacreous layer of oyster pearls.";
RL   Mar. Biotechnol. 2:409-418(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mantle;
RA   Hayashi N., Samata T.;
RT   "14 kD matrix protein family in nacreous layer of Japanese peal oyster,
RT   Pinctada fucata.";
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SUBUNIT, AND FUNCTION.
RX   PubMed=19679771; DOI=10.1126/science.1173793;
RA   Suzuki M., Saruwatari K., Kogure T., Yamamoto Y., Nishimura T., Kato T.,
RA   Nagasawa H.;
RT   "An acidic matrix protein, Pif, is a key macromolecule for nacre
RT   formation.";
RL   Science 325:1388-1390(2009).
RN   [4]
RP   FUNCTION.
RX   PubMed=19679772; DOI=10.1126/science.1177055;
RA   Kroger N.;
RT   "The molecular basis of nacre formation.";
RL   Science 325:1351-1352(2009).
CC   -!- FUNCTION: May be specifically involved in the formation of the nacreous
CC       layer. {ECO:0000250, ECO:0000269|PubMed:19679771,
CC       ECO:0000269|PubMed:19679772}.
CC   -!- SUBUNIT: Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other
CC       proteins form a complex. {ECO:0000269|PubMed:19679771}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix.
CC   -!- TISSUE SPECIFICITY: Component of conchiolin, the organic matrix of
CC       nacre. Specifically expressed in mantle epithelium.
CC   -!- SIMILARITY: Belongs to the N16 matrix protein family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=String of intrusion - Issue
CC       112 of December 2009;
CC       URL="https://web.expasy.org/spotlight/back_issues/112";
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DR   EMBL; AB020779; BAA75626.1; -; mRNA.
DR   EMBL; AB023252; BAA83737.1; -; mRNA.
DR   EMBL; AB023253; BAA83738.1; -; mRNA.
DR   EMBL; AB023254; BAA83739.1; -; mRNA.
DR   AlphaFoldDB; O97048; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Disulfide bond; Extracellular matrix; Repeat; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..129
FT                   /note="N16.5 matrix protein"
FT                   /id="PRO_0000379790"
FT   REPEAT          91..92
FT                   /note="1"
FT   REPEAT          93..94
FT                   /note="2"
FT   REPEAT          95..96
FT                   /note="3"
FT   REPEAT          97..98
FT                   /note="4"
FT   REPEAT          99..100
FT                   /note="5"
FT   REGION          91..100
FT                   /note="5 X 2 AA tandem repeats of N-G"
SQ   SEQUENCE   129 AA;  15388 MW;  6447712EA0940A9C CRC64;
     MTCTLRWTIT ALVLLGICHL ARPAFRTKCG RYSYCWIPYD IERDRYDNGD KKCCFCRNAW
     SPWQCKEDER YEWLRCGHKF YYMCCYTDDD NGNGNGNGNG FNYLKSLYGG YGNGNGEFWE
     EYIDERYDK