ID   MA658_ARATH             Reviewed;         562 AA.
AC   Q9C7G0;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=65-kDa microtubule-associated protein 8;
DE            Short=AtMAP65-8;
GN   Name=MAP65-8; OrderedLocusNames=At1g27920; ORFNames=F13K9.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12516862; DOI=10.1023/a:1021236307508;
RA   Hussey P.J., Hawkins T.J., Igarashi H., Kaloriti D., Smertenko A.;
RT   "The plant cytoskeleton: recent advances in the study of the plant
RT   microtubule-associated proteins MAP-65, MAP-190 and the Xenopus MAP215-like
RT   protein, MOR1.";
RL   Plant Mol. Biol. 50:915-924(2002).
RN   [4]
RP   INTERACTION WITH MICROTUBULES, AND SUBCELLULAR LOCATION.
RX   PubMed=15469496; DOI=10.1111/j.1365-313x.2004.02222.x;
RA   Van Damme D., Bouget F.-Y., Van Poucke K., Inze D., Geelen D.;
RT   "Molecular dissection of plant cytokinesis and phragmoplast structure: a
RT   survey of GFP-tagged proteins.";
RL   Plant J. 40:386-398(2004).
CC   -!- SUBUNIT: Forms a dimer (By similarity). Binds to microtubules (MT).
CC       Bundles polymerized MT via the formation of 25-nm crossbridges
CC       following an intracellular dotted lines pattern and at the minus-end of
CC       phragmoplast MT near the nuclear surface. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15469496}. Cytoplasm
CC       {ECO:0000269|PubMed:15469496}. Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000269|PubMed:15469496}. Note=Present in MT cortical arrays.
CC       Concentrated in dots surrounding the nuclei.
CC   -!- SIMILARITY: Belongs to the MAP65/ASE1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51477.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC069471; AAG51477.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE30891.1; -; Genomic_DNA.
DR   PIR; E86404; E86404.
DR   RefSeq; NP_174113.2; NM_102557.3.
DR   AlphaFoldDB; Q9C7G0; -.
DR   SMR; Q9C7G0; -.
DR   STRING; 3702.AT1G27920.1; -.
DR   PaxDb; Q9C7G0; -.
DR   PRIDE; Q9C7G0; -.
DR   ProteomicsDB; 238872; -.
DR   EnsemblPlants; AT1G27920.1; AT1G27920.1; AT1G27920.
DR   GeneID; 839685; -.
DR   Gramene; AT1G27920.1; AT1G27920.1; AT1G27920.
DR   KEGG; ath:AT1G27920; -.
DR   Araport; AT1G27920; -.
DR   TAIR; locus:2010434; AT1G27920.
DR   eggNOG; KOG4302; Eukaryota.
DR   HOGENOM; CLU_011760_1_0_1; -.
DR   InParanoid; Q9C7G0; -.
DR   OMA; WMASCDE; -.
DR   OrthoDB; 272248at2759; -.
DR   PhylomeDB; Q9C7G0; -.
DR   PRO; PR:Q9C7G0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C7G0; baseline and differential.
DR   Genevisible; Q9C7G0; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005874; C:microtubule; HDA:TAIR.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0009524; C:phragmoplast; HDA:TAIR.
DR   GO; GO:0005819; C:spindle; HDA:TAIR.
DR   GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR   GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   InterPro; IPR007145; MAP65_Ase1_PRC1.
DR   PANTHER; PTHR19321; PTHR19321; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Microtubule; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..562
FT                   /note="65-kDa microtubule-associated protein 8"
FT                   /id="PRO_0000395479"
FT   REGION          542..562
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          61..81
FT                   /evidence="ECO:0000255"
FT   COILED          155..190
FT                   /evidence="ECO:0000255"
FT   COILED          299..324
FT                   /evidence="ECO:0000255"
FT   COILED          361..381
FT                   /evidence="ECO:0000255"
FT   COILED          465..494
FT                   /evidence="ECO:0000255"
FT   SITE            426
FT                   /note="Microtubule binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  64603 MW;  FF80829D018CD06E CRC64;
     MGSLQTPIEM RSSSLLDTSC GYLLRELQMI WDEVGEDKFE REKVLLDIEQ ECVEAYRRKV
     DHANVSRSRL HQELAESEAE LTHFLLCLGE RSVPGRPEKK GGTLREQLDS IAPALREMRL
     RKDERVKQFR SVKGEIQKIS AEIAGRSTYE DSTRKITIDD NDLSNKKLEE YQNELHRLHD
     EKNERLQKVD IYICAIRDLS ATLGTEASMI ITKIHPSLND LYGISKNISD DILKKLNGTV
     VSLEEEKHKR LEKLHHLGRA LSNLWNLMDA SYEDRQKFFH VIDLLSSAPS DVCAPGSITL
     DIIQQAEAEV KRLDQLKASR IKELFIKKQK ELEDTCNMSH METPSTEMGN ITNLVDSGEV
     DHVDLLAAMD EKIARAKEEA ASRKGIIEKV DRWMLASDEE RWLEEYDQDE NRYSVSRNAH
     RNLRRAERAR ITVSKISGLV ESILVKAKSW EVERQKVFLY NEVPLVAMLQ EYNKLRQEKE
     MEKQRLREMK KMSIPQPVAE GDNFYMARPA SSNRRISNRS MNGGFGSGSP INRKYSGGFN
     NTNNNYTALG TSIRRESRKS EA