MA7D3_MOUSE
ID MA7D3_MOUSE Reviewed; 876 AA.
AC A2AEY4; Q0VF65; Q8C7V9; Q8C857; Q8CDD4;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=MAP7 domain-containing protein 3;
GN Name=Map7d3; Synonyms=Mdp3 {ECO:0000303|PubMed:22142902}, Mtap7d3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-519 (ISOFORM 2), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 22-876 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryonic head, Embryonic stem cell, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 22-876.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=22142902; DOI=10.4161/cc.10.22.18106;
RA Sun X., Shi X., Liu M., Li D., Zhang L., Liu X., Zhou J.;
RT "Mdp3 is a novel microtubule-binding protein that regulates microtubule
RT assembly and stability.";
RL Cell Cycle 10:3929-3937(2011).
CC -!- FUNCTION: Promotes the assembly and stability of microtubules.
CC {ECO:0000250|UniProtKB:Q8IWC1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q8IWC1}. Note=Localizes to the microtubules
CC throughout mitosis. {ECO:0000250|UniProtKB:Q8IWC1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2AEY4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AEY4-2; Sequence=VSP_028500;
CC -!- TISSUE SPECIFICITY: High expression in lung, skeletal muscle, brain,
CC and kidney, with much weaker expression in spleen, small intestine,
CC liver, and heart. {ECO:0000269|PubMed:22142902}.
CC -!- SIMILARITY: Belongs to the MAP7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI18964.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC26846.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC33317.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAM26746.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL671998; CAM26745.1; -; Genomic_DNA.
DR EMBL; AL671998; CAM26746.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK030211; BAC26846.1; ALT_INIT; mRNA.
DR EMBL; AK048385; BAC33317.1; ALT_INIT; mRNA.
DR EMBL; AK049164; BAC33580.1; -; mRNA.
DR EMBL; BC118963; AAI18964.1; ALT_INIT; mRNA.
DR CCDS; CCDS40982.1; -. [A2AEY4-1]
DR RefSeq; NP_796267.2; NM_177293.3. [A2AEY4-1]
DR AlphaFoldDB; A2AEY4; -.
DR SMR; A2AEY4; -.
DR BioGRID; 236396; 1.
DR STRING; 10090.ENSMUSP00000110414; -.
DR iPTMnet; A2AEY4; -.
DR PhosphoSitePlus; A2AEY4; -.
DR jPOST; A2AEY4; -.
DR MaxQB; A2AEY4; -.
DR PaxDb; A2AEY4; -.
DR PeptideAtlas; A2AEY4; -.
DR PRIDE; A2AEY4; -.
DR ProteomicsDB; 287290; -. [A2AEY4-1]
DR ProteomicsDB; 287291; -. [A2AEY4-2]
DR Antibodypedia; 51701; 20 antibodies from 10 providers.
DR DNASU; 320923; -.
DR Ensembl; ENSMUST00000114766; ENSMUSP00000110414; ENSMUSG00000067878. [A2AEY4-1]
DR GeneID; 320923; -.
DR KEGG; mmu:320923; -.
DR UCSC; uc009tgp.1; mouse. [A2AEY4-1]
DR UCSC; uc009tgq.1; mouse. [A2AEY4-2]
DR CTD; 79649; -.
DR MGI; MGI:2445051; Map7d3.
DR VEuPathDB; HostDB:ENSMUSG00000067878; -.
DR eggNOG; ENOG502SDH7; Eukaryota.
DR GeneTree; ENSGT00950000182941; -.
DR HOGENOM; CLU_016292_0_0_1; -.
DR InParanoid; A2AEY4; -.
DR OMA; WSWEGSA; -.
DR OrthoDB; 441248at2759; -.
DR PhylomeDB; A2AEY4; -.
DR TreeFam; TF332273; -.
DR BioGRID-ORCS; 320923; 3 hits in 71 CRISPR screens.
DR PRO; PR:A2AEY4; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; A2AEY4; protein.
DR Bgee; ENSMUSG00000067878; Expressed in epiblast (generic) and 40 other tissues.
DR ExpressionAtlas; A2AEY4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0015630; C:microtubule cytoskeleton; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0046785; P:microtubule polymerization; ISS:UniProtKB.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..876
FT /note="MAP7 domain-containing protein 3"
FT /id="PRO_0000306813"
FT REGION 30..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 402..438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..683
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 742..783
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 58..136
FT /evidence="ECO:0000255"
FT COILED 549..578
FT /evidence="ECO:0000255"
FT COILED 626..658
FT /evidence="ECO:0000255"
FT COMPBIAS 42..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..139
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..194
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..587
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 598..612
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..664
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 768..783
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 417
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWC1"
FT MOD_RES 483
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWC1"
FT VAR_SEQ 202..231
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028500"
FT CONFLICT 217
FT /note="S -> C (in Ref. 2; BAC26846)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 876 AA; 98230 MW; BF11198ABE63267C CRC64;
MADPTFAATS SSTSFRGLHE RLVAMTQELA EERRHKRGIN SSAGANKRSS STPDGSVLKN
DVKQQLAKER REQQKRQQEA NKEKQLLEKE QKAKLQYEKQ LEEKHRKLKE QKEKDQRRQA
SAEEKRKQKQ AEDTEKFKAV VSRTLERCNR IDQRQKRWSW EGGVMNADKS GKLENKRSSS
LSRKDNRLHP RGDMQHVDNT PGMTKYVFRY VTAPVFSSDE IKSSAMFCKP SAKTPVAAKL
EKITTKKLDA SLRGHVEGLS MMNIEIPPKI TIEVPSPPKL EESSEADAEV RLQTMDDISK
VKEDASQKVD IKVPTDENIA RHPKPNVEEL SPVSVDTSSS VELSSIVSVN SSPSLSTGSF
SFGSVEISPV VSIDASLETN IDTSPELSMD SGNTKVASEI KTEAPLQARG ESRLEASVEG
QPEANVEGSP KNPEIDKRNI NLTTKKQPLC HIPCYRWPSS SALGCRPPSP LKALQTRKIR
PPSPIPVSSK LSTKTSLSYK ITPVQNVLYV PNSLGVIATK KETIQKYPIK KEFGNRSMPS
AEAIKKAFIQ IRHAAYEQSK NEKERLQKEE TKQRIARKPE IMAEKLDKVP AEGSLPCQDE
QQDKNPTKTF LESPEVQKAE LQKGDSAMMK SRDSAEQRKK EQENILQHWQ ERLERRKASE
ISFSSEDEAD DEGESEDSLE IFPSGGKMLS MKLKKFHKYA KTKPQKLVFL QSGTDEVDTN
KNVYFNGDMK AVKQKDPKYS MIQGKGSKLS AKKPPTRPIR SRKTKEGSTA IRPTQSASSN
PNHKWVCDKV IDFNQTPFLK TTLTKSNKES PADSKIACQG PQAHLDHRKR TKSVSVPLTN
VLSHLHITGR ASNLEHPFAS VYSRLAFGKE AEESDV
