MAA3_ARATH
ID MAA3_ARATH Reviewed; 818 AA.
AC B6SFA4; O23408;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Probable helicase MAGATAMA 3;
DE EC=3.6.4.-;
DE AltName: Full=SEN1-like protein;
GN Name=MAA3; OrderedLocusNames=At4g15570; ORFNames=dl3825w;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Wassilewskija-2;
RX PubMed=18772186; DOI=10.1093/pcp/pcn130;
RA Shimizu K.K., Ito T., Ishiguro S., Okada K.;
RT "MAA3 (MAGATAMA3) helicase gene is required for female gametophyte
RT development and pollen tube guidance in Arabidopsis thaliana.";
RL Plant Cell Physiol. 49:1478-1483(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija-2;
RX PubMed=11003848; DOI=10.1242/dev.127.20.4511;
RA Shimizu K.K., Okada K.;
RT "Attractive and repulsive interactions between female and male gametophytes
RT in Arabidopsis pollen tube guidance.";
RL Development 127:4511-4518(2000).
RN [6]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- FUNCTION: Probable helicase that may regulate RNA molecules involved in
CC nucleolar organization and pollen tube guidance.
CC {ECO:0000269|PubMed:11003848, ECO:0000269|PubMed:18772186}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, siliques, leaves, roots and
CC shoot apex. {ECO:0000269|PubMed:18772186}.
CC -!- DISRUPTION PHENOTYPE: Defective in female gametophyte development and
CC micropylar pollen tube guidance leading to zygotic lethality. Reduced
CC size of nucleoli of polar nuclei in female gametophyte.
CC {ECO:0000269|PubMed:11003848}.
CC -!- SIMILARITY: Belongs to the helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB10335.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78599.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EU915246; ACI63222.1; -; mRNA.
DR EMBL; Z97339; CAB10335.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161542; CAB78599.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83627.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67158.1; -; Genomic_DNA.
DR PIR; E71420; E71420.
DR RefSeq; NP_001329005.1; NM_001341064.1.
DR RefSeq; NP_193292.3; NM_117648.8.
DR AlphaFoldDB; B6SFA4; -.
DR SMR; B6SFA4; -.
DR BioGRID; 12529; 16.
DR STRING; 3702.AT4G15570.1; -.
DR iPTMnet; B6SFA4; -.
DR PaxDb; B6SFA4; -.
DR PRIDE; B6SFA4; -.
DR ProteomicsDB; 238229; -.
DR EnsemblPlants; AT4G15570.1; AT4G15570.1; AT4G15570.
DR EnsemblPlants; AT4G15570.3; AT4G15570.3; AT4G15570.
DR GeneID; 827233; -.
DR Gramene; AT4G15570.1; AT4G15570.1; AT4G15570.
DR Gramene; AT4G15570.3; AT4G15570.3; AT4G15570.
DR KEGG; ath:AT4G15570; -.
DR Araport; AT4G15570; -.
DR TAIR; locus:2130384; AT4G15570.
DR eggNOG; KOG1801; Eukaryota.
DR HOGENOM; CLU_001666_11_2_1; -.
DR InParanoid; B6SFA4; -.
DR OrthoDB; 62494at2759; -.
DR PhylomeDB; B6SFA4; -.
DR PRO; PR:B6SFA4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; B6SFA4; baseline and differential.
DR Genevisible; B6SFA4; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0010183; P:pollen tube guidance; IMP:TAIR.
DR GO; GO:0009875; P:pollen-pistil interaction; IMP:TAIR.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10887; PTHR10887; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..818
FT /note="Probable helicase MAGATAMA 3"
FT /id="PRO_0000416787"
FT DOMAIN 259..559
FT /note="UvrD-like helicase ATP-binding"
FT REGION 781..818
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 801..818
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 280..287
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 818 AA; 91540 MW; 9E9CDD46DBF30018 CRC64;
MAIDNGKLQE EEASSVTRFY NIILGWDYKQ LTKENERKNR KDSKEKLNVV KNTYKDVDDY
FETFEPLLFE EVKAQILQNK DGEEASVCKM RLVMECNEGE GFHFLLVTYE HEEDEYLAQN
DLLLLSKEEV KGNSFPSSYG FAVVEHRQNN LLRLRMYLAE DIVQITKNTK SSRTKSFIQA
LSNMRSLITS SASPIDKRVF SLKLCGLSTI IREYIALRSV SSLPFKDLIF TAAEKSCGFG
DEAWKISGPL NEFFNENLNK SQKEAIDVGL SRKSFVLIQG PPGTGKTQTI LSILGAIMHA
TPARVQSKGT DHEVKRGIQM TIQEKYNHWG RASPWILGVN PRDAIMPEDG DDGFFPTSGN
ELKPEVVNAS RKYRLRVLVC APSNSALDEI VLRLLSSGLR DENAQTYTPK IVRIGLKAHH
SVASVSLDHL VAQKRGSAID KPKQGTTGTD IDSIRTAILE EAAIVFATLS FSGSALLAKS
NRGFDVVIID EAAQAVEPAT LIPLATRCKQ VFLVGDPKQL PATVISTVAQ DSGYGTSMFE
RLQKAGYPVK MLKTQYRMHP EIRSFPSKQF YEGALEDGSD IEAQTTRDWH KYRCFGPFCF
FDIHEGKESQ HPGATGSRVN LDEVEFVLLI YHRLVTMYPE LKSSSQLAII SPYNYQVKTF
KDRFKEMFGT EAEKVVDINT VDGFQGREKD VAIFSCVRAN ENGQIGFLSN SRRMNVGITR
AKSSVLVVGS AATLKSDPLW KNLIESAEQR NRLFKVSKPL NNFFSEENLE TMKLTEDMEI
PDAPLYEDES LPVAPYGGDD DFGDGDADQD DVAMAGED
