MAB23_CAEEL
ID MAB23_CAEEL Reviewed; 175 AA.
AC G5ECK3; Q1NZ22;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=DM domain-containing protein mab-23 {ECO:0000312|WormBase:C32C4.5a};
GN Name=mab-23 {ECO:0000312|WormBase:C32C4.5a};
GN ORFNames=C32C4.5 {ECO:0000312|WormBase:C32C4.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAN17799.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF CYS-38.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAN17799.1};
RX PubMed=12231628; DOI=10.1101/gad.1012602;
RA Lints R., Emmons S.W.;
RT "Regulation of sex-specific differentiation and mating behavior in C.
RT elegans by a new member of the DM domain transcription factor family.";
RL Genes Dev. 16:2390-2402(2002).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=22069471; DOI=10.1371/journal.pone.0026811;
RA Siehr M.S., Koo P.K., Sherlekar A.L., Bian X., Bunkers M.R., Miller R.M.,
RA Portman D.S., Lints R.;
RT "Multiple doublesex-related genes specify critical cell fates in a C.
RT elegans male neural circuit.";
RL PLoS ONE 6:E26811-E26811(2011).
CC -!- FUNCTION: Probable transcription factor that plays a role in the
CC development of the dopaminergic neurons of the male-specific genital
CC sensilla (simple sense organs) known as rays, by negatively regulating
CC the activity of the transcription factor ast-1 (PubMed:22069471,
CC PubMed:12231628). Involved in male mating behavior, probably as a
CC result of a role in the differentiation of male-specific diagonal
CC muscles (PubMed:12231628, PubMed:22069471). Required for development of
CC the male proctodeum (PubMed:12231628). May be dispensable in
CC hermaphrodites (PubMed:12231628). {ECO:0000269|PubMed:12231628,
CC ECO:0000269|PubMed:22069471}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:C32C4.5a};
CC IsoId=G5ECK3-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C32C4.5b};
CC IsoId=G5ECK3-2; Sequence=VSP_061377;
CC -!- TISSUE SPECIFICITY: Expressed in a limited number of non-sex-specific
CC tissues in males, including 6-8 unidentified neurons of the head,
CC ventral body wall muscle, and the PHCL/R neurons.
CC {ECO:0000269|PubMed:12231628}.
CC -!- DEVELOPMENTAL STAGE: In males, expressed in several sex-specific cell
CC types during larval development and in the adult, including the A-type
CC ray sensory neurons, ventral male-specific muscles, and unidentified
CC neurons of the male posterior ventral nerve cord (PubMed:12231628).
CC Transiently expressed during larval development in the hindgut
CC (PubMed:12231628). {ECO:0000269|PubMed:12231628}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes defects in
CC patterning of the dopaminergic neurons of the male-specific genital
CC sensilla (simple sense organs) known as rays (PubMed:12231628). Males
CC are unable to sire progeny due to abnormal tail and gonad morphology,
CC whereas hermaphrodites have no obvious phenotypic defects
CC (PubMed:12231628). {ECO:0000269|PubMed:12231628}.
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DR EMBL; AF535153; AAN17799.1; -; mRNA.
DR EMBL; BX284605; CAD44103.1; -; Genomic_DNA.
DR EMBL; BX284605; CAJ90497.1; -; Genomic_DNA.
DR RefSeq; NP_001041089.1; NM_001047624.1.
DR AlphaFoldDB; G5ECK3; -.
DR IntAct; G5ECK3; 1.
DR STRING; 6239.C32C4.5a; -.
DR PaxDb; G5ECK3; -.
DR EnsemblMetazoa; C32C4.5a.1; C32C4.5a.1; WBGene00003114. [G5ECK3-1]
DR EnsemblMetazoa; C32C4.5b.1; C32C4.5b.1; WBGene00003114. [G5ECK3-2]
DR GeneID; 266926; -.
DR KEGG; cel:CELE_C32C4.5; -.
DR CTD; 266926; -.
DR WormBase; C32C4.5a; CE31427; WBGene00003114; mab-23.
DR WormBase; C32C4.5b; CE40025; WBGene00003114; mab-23.
DR eggNOG; ENOG502STJF; Eukaryota.
DR HOGENOM; CLU_106428_0_0_1; -.
DR InParanoid; G5ECK3; -.
DR OMA; IFHSAEM; -.
DR OrthoDB; 1588837at2759; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003114; Expressed in larva and 1 other tissue.
DR ExpressionAtlas; G5ECK3; baseline.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0048664; P:neuron fate determination; IMP:UniProtKB.
DR GO; GO:1902435; P:regulation of male mating behavior; IMP:UniProtKB.
DR GO; GO:0110037; P:regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IBA:GO_Central.
DR Gene3D; 4.10.1040.10; -; 1.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR026607; DMRT.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 1.
DR SMART; SM00301; DM; 1.
DR SUPFAM; SSF82927; SSF82927; 1.
DR PROSITE; PS50809; DM_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Zinc.
FT CHAIN 1..175
FT /note="DM domain-containing protein mab-23"
FT /id="PRO_0000454716"
FT DNA_BIND 8..56
FT /note="DM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 58..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..105
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061377"
FT MUTAGEN 38
FT /note="C->F: In e2518; defects in patterning of the
FT dopaminergic neurons of the male-specific genital sensilla
FT (simple sense organs) known as rays. Males abnormally
FT express dopamine transporter cat-2 and synthesize dopamine
FT in the A-type neurons of the rays. Males are unable to sire
FT progeny due to abnormal tail and gonad morphology, whereas
FT hermaphrodites have no obvious phenotypic defects."
FT /evidence="ECO:0000269|PubMed:12231628"
SQ SEQUENCE 175 AA; 19550 MW; 3626085B739FE443 CRC64;
MPKEQYMCQL CANHGIFNQP KKGHKQKCPY RTCPCSLCAL NTKRRALDQI ERQLKHTNEP
MTGQTATSMA SPTPECPLSP TTPKMTPHTP TSGKDTFRNS ISSSNMAFTV QLPATITKKE
LKLLRRDDTP LQNSLERPFP RSIDEAIETI KKEKMSSIFH SAEMLAVGES ATSLI
