MAB3_CAEEL
ID MAB3_CAEEL Reviewed; 290 AA.
AC O18214; Q8I0Z0;
DT 08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein male abnormal 3;
GN Name=mab-3 {ECO:0000312|WormBase:Y53C12B.5a};
GN ORFNames=Y53C12B.5 {ECO:0000312|WormBase:Y53C12B.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=9490411; DOI=10.1038/35618;
RA Raymond C.S., Shamu C.E., Shen M.M., Seifert K.J., Hirsch B., Hodgkin J.,
RA Zarkower D.;
RT "Evidence for evolutionary conservation of sex-determining genes.";
RL Nature 391:691-695(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=18550714; DOI=10.1242/dev.017046;
RA Mason D.A., Rabinowitz J.S., Portman D.S.;
RT "dmd-3, a doublesex-related gene regulated by tra-1, governs sex-specific
RT morphogenesis in C. elegans.";
RL Development 135:2373-2382(2008).
RN [4]
RP FUNCTION.
RX PubMed=21408209; DOI=10.1371/journal.pgen.1002010;
RA Nelson M.D., Zhou E., Kiontke K., Fradin H., Maldonado G., Martin D.,
RA Shah K., Fitch D.H.;
RT "A bow-tie genetic architecture for morphogenesis suggested by a genome-
RT wide RNAi screen in Caenorhabditis elegans.";
RL PLoS Genet. 7:E1002010-E1002010(2011).
RN [5]
RP REPRESSION BY LIN-41.
RX PubMed=28111013; DOI=10.1016/j.molcel.2016.12.010;
RA Aeschimann F., Kumari P., Bartake H., Gaidatzis D., Xu L., Ciosk R.,
RA Grosshans H.;
RT "LIN41 post-transcriptionally silences mRNAs by two distinct and position-
RT dependent mechanisms.";
RL Mol. Cell 65:476-489(2017).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=31264582; DOI=10.7554/elife.43660;
RA Lawson H., Vuong E., Miller R.M., Kiontke K., Fitch D.H., Portman D.S.;
RT "The Makorin lep-2 and the lncRNA lep-5 regulate lin-28 to schedule sexual
RT maturation of the C. elegans nervous system.";
RL Elife 8:0-0(2019).
CC -!- FUNCTION: Transcription factor which binds the DNA motif 5'-
CC [CGA][TCA][TA]ACAATGT[AT][TGA]C-3', probably as a monomer
CC (PubMed:9490411). Acts partially redundantly with the transcription
CC factor dmd-3 to coordinate tail tip cell fusion and retraction and
CC thereby regulate male tail tip morphogenesis (PubMed:18550714,
CC PubMed:21408209). Promotes male-specific development of two tissues,
CC the peripheral nervous system and the intestine (PubMed:9490411). In
CC the peripheral nervous system, directs differentiation of sensory ray
CC neuroblasts into peripheral sense organs (PubMed:9490411). In the
CC intestine, causes repression of vitellogenin gene transcription
CC (PubMed:9490411). {ECO:0000269|PubMed:18550714,
CC ECO:0000269|PubMed:21408209, ECO:0000269|PubMed:9490411}.
CC -!- INTERACTION:
CC O18214; Q10666: pop-1; NbExp=3; IntAct=EBI-2420993, EBI-317870;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00070}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:Y53C12B.5a};
CC IsoId=O18214-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y53C12B.5b};
CC IsoId=O18214-3; Sequence=VSP_060330;
CC -!- TISSUE SPECIFICITY: Expression is undetectable in hermaphrodites, but
CC persists in males (PubMed:9490411). In males, expressed in cells of the
CC tail tip (PubMed:18550714). {ECO:0000269|PubMed:18550714,
CC ECO:0000269|PubMed:9490411}.
CC -!- DEVELOPMENTAL STAGE: Expressed in late larvae of both sexes. In the
CC fourth larval stage, expression is approximately six-fold higher in
CC males than in hermaphrodites. Expressed in ADF chemosensory neurons in
CC males from the L4 larval stage (PubMed:31264582). Expressed in cells of
CC the male tail tip from the L4 larval stage (PubMed:18550714). Not
CC expressed in cells of the hermaphrodite tail tip at any developmental
CC stage (PubMed:18550714). {ECO:0000269|PubMed:18550714,
CC ECO:0000269|PubMed:31264582, ECO:0000269|PubMed:9490411}.
CC -!- INDUCTION: Negatively regulated by lin-41 which causes degradation of
CC the mRNA encoding this protein. {ECO:0000269|PubMed:18550714,
CC ECO:0000269|PubMed:28111013}.
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DR EMBL; AF022388; AAC38956.1; -; Genomic_DNA.
DR EMBL; BX284602; CAB16489.1; -; Genomic_DNA.
DR EMBL; BX284602; CAD59170.2; -; Genomic_DNA.
DR PIR; T27132; T27132.
DR RefSeq; NP_001022464.1; NM_001027293.1. [O18214-1]
DR RefSeq; NP_871909.2; NM_182109.4.
DR AlphaFoldDB; O18214; -.
DR SMR; O18214; -.
DR BioGRID; 39855; 55.
DR IntAct; O18214; 55.
DR STRING; 6239.Y53C12B.5a; -.
DR PaxDb; O18214; -.
DR PRIDE; O18214; -.
DR EnsemblMetazoa; Y53C12B.5a.1; Y53C12B.5a.1; WBGene00003100. [O18214-1]
DR EnsemblMetazoa; Y53C12B.5b.1; Y53C12B.5b.1; WBGene00003100. [O18214-3]
DR EnsemblMetazoa; Y53C12B.5b.2; Y53C12B.5b.2; WBGene00003100. [O18214-3]
DR GeneID; 174533; -.
DR KEGG; cel:CELE_Y53C12B.5; -.
DR UCSC; Y53C12B.5a; c. elegans. [O18214-1]
DR CTD; 174533; -.
DR WormBase; Y53C12B.5a; CE14902; WBGene00003100; mab-3. [O18214-1]
DR WormBase; Y53C12B.5b; CE51560; WBGene00003100; mab-3. [O18214-3]
DR eggNOG; KOG3815; Eukaryota.
DR HOGENOM; CLU_934575_0_0_1; -.
DR InParanoid; O18214; -.
DR OMA; RCLNHGE; -.
DR OrthoDB; 1727673at2759; -.
DR SignaLink; O18214; -.
DR PRO; PR:O18214; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003100; Expressed in adult organism and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0110039; P:positive regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IMP:WormBase.
DR Gene3D; 4.10.1040.10; -; 2.
DR InterPro; IPR001275; DM_DNA-bd.
DR InterPro; IPR036407; DM_DNA-bd_sf.
DR InterPro; IPR026607; DMRT.
DR PANTHER; PTHR12322; PTHR12322; 1.
DR Pfam; PF00751; DM; 2.
DR SMART; SM00301; DM; 2.
DR SUPFAM; SSF82927; SSF82927; 2.
DR PROSITE; PS40000; DM_1; 2.
DR PROSITE; PS50809; DM_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Sexual differentiation;
KW Zinc.
FT CHAIN 1..290
FT /note="Protein male abnormal 3"
FT /id="PRO_0000207051"
FT DNA_BIND 28..74
FT /note="DM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT DNA_BIND 94..142
FT /note="DM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00070"
FT REGION 139..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..166
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..133
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060330"
SQ SEQUENCE 290 AA; 32463 MW; 2F324FFB0B8846D5 CRC64;
MLTEDPVSEI CEAKAVDELA EQEKNYYCQR CLNHGELKPR KGHKPDCRYL KCPCRECTMV
EQRRQLNNLL SKKKIHCTPA TQTRDGKRVR DPHCARCSAH GVLVPLRGHK RTMCQFVTCE
CTLCTLVEHR RNLMAAQIKL RRSQQKSRDG KEPKRNSRRK SKDMDMEMMV VTATDGQKII
GTSASPSPSS TTDTMSPSLS MSPPCSPSPL LAQYTLTLAA PIPIYPPIPM NQQLISLQQQ
QFLMSIIQNM APSIGQQAPL LPGISAGSVS SAAILNEFWS MYLKNYGLQA
