ID   MACA1_RHOOP             Reviewed;         357 AA.
AC   O84992;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Maleylacetate reductase 1;
DE            EC=1.3.1.32;
DE   AltName: Full=Maleylacetate reductase I;
GN   Name=macA;
OS   Rhodococcus opacus (Nocardia opaca).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=37919;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1CP;
RX   PubMed=9657989; DOI=10.1128/jb.180.14.3503-3508.1998;
RA   Seibert V., Kourbatova E.M., Golovleva L.A., Schloemann M.;
RT   "Characterization of the maleylacetate reductase MacA of Rhodococcus opacus
RT   1CP and evidence for the presence of an isofunctional enzyme.";
RL   J. Bacteriol. 180:3503-3508(1998).
CC   -!- FUNCTION: Plays a major role in the degradation of chloroaromatic
CC       compounds by channeling maleylacetate and some of its substituted
CC       derivatives into the 3-oxoadipate pathway. This enzyme converts
CC       maleylacetate and 2-chloromaleylacetate with similar efficiencies.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NAD(+) = H(+) + maleylacetate + NADH;
CC         Xref=Rhea:RHEA:16981, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.32;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NADP(+) = H(+) + maleylacetate + NADPH;
CC         Xref=Rhea:RHEA:16985, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.32;
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; AF030176; AAC38802.1; -; Genomic_DNA.
DR   AlphaFoldDB; O84992; -.
DR   SMR; O84992; -.
DR   BioCyc; MetaCyc:MON-13024; -.
DR   UniPathway; UPA00083; -.
DR   GO; GO:0018506; F:maleylacetate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08177; MAR; 1.
DR   InterPro; IPR001670; ADH_Fe/GldA.
DR   InterPro; IPR039697; Alcohol_dehydrogenase_Fe.
DR   InterPro; IPR034786; MAR.
DR   PANTHER; PTHR11496; PTHR11496; 1.
DR   Pfam; PF00465; Fe-ADH; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..357
FT                   /note="Maleylacetate reductase 1"
FT                   /id="PRO_0000087847"
SQ   SEQUENCE   357 AA;  37798 MW;  05BEFE211A7249CF CRC64;
     MAERSSEFVF TGNPARVIFG AGRMRNVREE VERLGRGRVL LLGSENLREV CDQVQDLLGE
     LFVNRYDGAA MHTPVEVTDI ALAQLRTSEA DCVVAIGGGS TTGLAKALAA RTGVDQVILP
     TTYAGSEVTP VLGETVEGRK TTRSTLAVLP ETVIYDVELS KNLPVPIAVA SAVNALAHAV
     EAMYSPDANP VVDTWALEAA QALARGLRGL VSDPSCRRIR TDLLRGSWLA GMCLGSVGMA
     VHHKLCHTLG GAFGLPHAPT HTVVLPYAMS FNASEVPDVM DSLASAMNVS NAPAGVWDLI
     ADAGGPTSLA SLGLLQTDLD RAADLATEAP YRNPRQITRS GIRDLLQSAW EGNRPPE