ID   MACA2_RHOOP             Reviewed;          15 AA.
AC   P56870;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   02-DEC-2020, entry version 46.
DE   RecName: Full=Putative maleylacetate reductase 2;
DE            EC=1.3.1.32;
DE   AltName: Full=maleylacetate reductase II;
DE   Flags: Fragment;
OS   Rhodococcus opacus (Nocardia opaca).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=37919;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=1CP;
RX   PubMed=9657989; DOI=10.1128/jb.180.14.3503-3508.1998;
RA   Seibert V., Kourbatova E.M., Golovleva L.A., Schloemann M.;
RT   "Characterization of the maleylacetate reductase MacA of Rhodococcus opacus
RT   1CP and evidence for the presence of an isofunctional enzyme.";
RL   J. Bacteriol. 180:3503-3508(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NAD(+) = H(+) + maleylacetate + NADH;
CC         Xref=Rhea:RHEA:16981, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.32;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxoadipate + NADP(+) = H(+) + maleylacetate + NADPH;
CC         Xref=Rhea:RHEA:16985, ChEBI:CHEBI:15378, ChEBI:CHEBI:15775,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.32;
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   UniPathway; UPA00083; -.
DR   GO; GO:0018506; F:maleylacetate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; NAD;
KW   Oxidoreductase.
FT   CHAIN           1..>15
FT                   /note="Putative maleylacetate reductase 2"
FT                   /id="PRO_0000087848"
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1885 MW;  58DA90DD038F025E CRC64;
     MRFEHENLPQ RIXFG