MACA_ECO57
ID MACA_ECO57 Reviewed; 371 AA.
AC P64176; P58410;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Macrolide export protein MacA;
GN Name=macA; OrderedLocusNames=Z1115, ECs0964;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Part of the tripartite efflux system MacAB-TolC. MacA
CC stimulates the ATPase activity of MacB by promoting the closed ATP-
CC bound state of MacB, increases the capacity of MacB to bind macrolides
CC such as erythromycin, and provides a physical link between MacB and
CC TolC. Confers resistance against macrolides (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homohexamer. Part of the tripartite efflux system MacAB-TolC,
CC which is composed of an inner membrane transporter, MacB, a periplasmic
CC membrane fusion protein, MacA, and an outer membrane component, TolC.
CC The complex forms a large protein conduit and can translocate molecules
CC across both the inner and outer membranes. MacA interacts with MacB and
CC TolC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC membrane protein {ECO:0000250}; Periplasmic side {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG55260.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB34387.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG55260.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB34387.1; ALT_INIT; Genomic_DNA.
DR PIR; D90749; D90749.
DR PIR; H85599; H85599.
DR RefSeq; NP_308991.2; NC_002695.1.
DR RefSeq; WP_000746446.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P64176; -.
DR SMR; P64176; -.
DR STRING; 155864.EDL933_1012; -.
DR EnsemblBacteria; AAG55260; AAG55260; Z1115.
DR EnsemblBacteria; BAB34387; BAB34387; ECs_0964.
DR GeneID; 58389704; -.
DR GeneID; 917702; -.
DR KEGG; ece:Z1115; -.
DR KEGG; ecs:ECs_0964; -.
DR PATRIC; fig|386585.9.peg.1080; -.
DR eggNOG; COG0845; Bacteria.
DR HOGENOM; CLU_018816_14_1_6; -.
DR OMA; TGKIQPE; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0019898; C:extrinsic component of membrane; IEA:InterPro.
DR GO; GO:1990195; C:macrolide transmembrane transporter complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR030190; MacA.
DR InterPro; IPR006143; RND_pump_MFP.
DR PANTHER; PTHR30469:SF34; PTHR30469:SF34; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR TIGRFAMs; TIGR01730; RND_mfp; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Coiled coil;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..371
FT /note="Macrolide export protein MacA"
FT /id="PRO_0000018696"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..371
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT COILED 92..137
FT /evidence="ECO:0000255"
SQ SEQUENCE 371 AA; 40639 MW; 25F7D3CB1A2D080F CRC64;
MKKRKTVKKR YVIALVIVIA GLITLWRILN APVPTYQTLI VRPGDLQQSV LATGKLDALR
KVDVGAQVSG QLKTLSVAIG DKVKKDQLLG VIDPEQAENQ IKEVEATLME LRAQRQQAEA
ELKLARVTYS RQQRLAQTQA VSLQDLDTAA TEMAVKQAQI GTIDAQIKRN QASLDTAKTN
LDYTRIVAPM AGEVTQITTL QGQTVIAAQQ APNILTLADM STMLVKAQVS EADVIHLKPG
QKAWFTVLGD PLTRYEGQIK DVLPTPEKVN DAIFYYARFE VPNPNGLLRL DMTAQVHIQL
TDVKNVLTIP LSALGDPVGD NRYKVKLLRN GETREREVTI GARNDTDVEI VKGLEAGDEV
VIGEAKPGAA Q