MACA_YERPE
ID MACA_YERPE Reviewed; 371 AA.
AC P58411; Q0WH51;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Macrolide export protein MacA;
GN Name=macA; OrderedLocusNames=YPO1364, y2814, YP_1230;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Part of the tripartite efflux system MacAB-TolC. MacA
CC stimulates the ATPase activity of MacB by promoting the closed ATP-
CC bound state of MacB, increases the capacity of MacB to bind macrolides
CC such as erythromycin, and provides a physical link between MacB and
CC TolC. Confers resistance against macrolides (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homohexamer. Part of the tripartite efflux system MacAB-TolC,
CC which is composed of an inner membrane transporter, MacB, a periplasmic
CC membrane fusion protein, MacA, and an outer membrane component, TolC.
CC The complex forms a large protein conduit and can translocate molecules
CC across both the inner and outer membranes. MacA interacts with MacB and
CC TolC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC membrane protein {ECO:0000250}; Periplasmic side {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAS61473.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL590842; CAL20016.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM86365.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS61473.1; ALT_INIT; Genomic_DNA.
DR PIR; AF0166; AF0166.
DR RefSeq; YP_002346387.1; NC_003143.1.
DR AlphaFoldDB; P58411; -.
DR SMR; P58411; -.
DR IntAct; P58411; 2.
DR STRING; 214092.YPO1364; -.
DR PaxDb; P58411; -.
DR DNASU; 1147761; -.
DR EnsemblBacteria; AAM86365; AAM86365; y2814.
DR EnsemblBacteria; AAS61473; AAS61473; YP_1230.
DR KEGG; ype:YPO1364; -.
DR KEGG; ypk:y2814; -.
DR KEGG; ypm:YP_1230; -.
DR PATRIC; fig|214092.21.peg.1685; -.
DR eggNOG; COG0845; Bacteria.
DR HOGENOM; CLU_018816_14_1_6; -.
DR OMA; ADYQIIR; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:1990281; C:efflux pump complex; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IEA:InterPro.
DR GO; GO:1990195; C:macrolide transmembrane transporter complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IEA:InterPro.
DR InterPro; IPR032317; HlyD_D23.
DR InterPro; IPR030190; MacA.
DR InterPro; IPR006143; RND_pump_MFP.
DR PANTHER; PTHR30469:SF34; PTHR30469:SF34; 1.
DR Pfam; PF16576; HlyD_D23; 1.
DR TIGRFAMs; TIGR01730; RND_mfp; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Coiled coil;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..371
FT /note="Macrolide export protein MacA"
FT /id="PRO_0000018698"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..371
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT COILED 94..127
FT /evidence="ECO:0000255"
SQ SEQUENCE 371 AA; 40186 MW; 13173F46A8696E3E CRC64;
MMQLSRGQRR WLAAIAVLLI GGFFIARHLM APVPVNYQTV KVVHRDLQQN VLATGKLDAV
RKVDVGAQVS GQLEKLYVEI GDHVKRGQLL AMIDPQQAQN QIKEVEATLQ DLNAQRIQAK
AELHLATVTL GRQQNLAKLQ VVSRQDLDQA VTDLAVKNAK VGTIDAQINK AKASLDTAKI
NLDYTQISAP MDGDVVQITT LQGQTVIAAQ QAPNILTLAD MSTMLVQAQV SEADVINLKP
GMKASFTVLG DPGKRFSGVL KDILPTPEKV NDAIFYSARF EVPNPDRLLR LQMTAQVSIQ
LANVDQAVVI PLAALGDELG SNRYQVTVLK EGKEEKREVT IGIRNNVDAQ VISGLSVGED
VIVSRGGTGD A
