MACB2_PSEE4
ID MACB2_PSEE4 Reviewed; 654 AA.
AC Q1I7I9;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Macrolide export ATP-binding/permease protein MacB 2 {ECO:0000255|HAMAP-Rule:MF_01720};
DE EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01720};
GN Name=macB2 {ECO:0000255|HAMAP-Rule:MF_01720}; OrderedLocusNames=PSEEN3665;
OS Pseudomonas entomophila (strain L48).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=384676;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L48;
RX PubMed=16699499; DOI=10.1038/nbt1212;
RA Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C.,
RA Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P.,
RA Weissenbach J., Lemaitre B., Medigue C., Boccard F.;
RT "Complete genome sequence of the entomopathogenic and metabolically
RT versatile soil bacterium Pseudomonas entomophila.";
RL Nat. Biotechnol. 24:673-679(2006).
CC -!- FUNCTION: Part of the tripartite efflux system MacAB-TolC. MacB is a
CC non-canonical ABC transporter that contains transmembrane domains
CC (TMD), which form a pore in the inner membrane, and an ATP-binding
CC domain (NBD), which is responsible for energy generation. Confers
CC resistance against macrolides. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC -!- SUBUNIT: Homodimer. Part of the tripartite efflux system MacAB-TolC,
CC which is composed of an inner membrane transporter, MacB, a periplasmic
CC membrane fusion protein, MacA, and an outer membrane component, TolC.
CC The complex forms a large protein conduit and can translocate molecules
CC across both the inner and outer membranes. Interacts with MacA.
CC {ECO:0000255|HAMAP-Rule:MF_01720}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01720}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01720}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Macrolide
CC exporter (TC 3.A.1.122) family. {ECO:0000255|HAMAP-Rule:MF_01720}.
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DR EMBL; CT573326; CAK16390.1; -; Genomic_DNA.
DR RefSeq; WP_011534771.1; NC_008027.1.
DR AlphaFoldDB; Q1I7I9; -.
DR SMR; Q1I7I9; -.
DR STRING; 384676.PSEEN3665; -.
DR PRIDE; Q1I7I9; -.
DR EnsemblBacteria; CAK16390; CAK16390; PSEEN3665.
DR KEGG; pen:PSEEN3665; -.
DR eggNOG; COG0577; Bacteria.
DR eggNOG; COG1136; Bacteria.
DR HOGENOM; CLU_000604_78_2_6; -.
DR OMA; VVILITH; -.
DR OrthoDB; 1181903at2; -.
DR Proteomes; UP000000658; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003838; ABC3_permease_dom.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR017911; MacB_ATP-bd.
DR InterPro; IPR025857; MacB_PCD.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF02687; FtsX; 1.
DR Pfam; PF12704; MacB_PCD; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51267; MACB; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; ATP-binding; Cell inner membrane; Cell membrane;
KW Membrane; Nucleotide-binding; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..654
FT /note="Macrolide export ATP-binding/permease protein MacB
FT 2"
FT /id="PRO_0000280173"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 617..637
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT DOMAIN 6..245
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT BINDING 43..50
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
SQ SEQUENCE 654 AA; 69628 MW; EF96C8CB11B25740 CRC64;
MSAPLIELCD IRKAYGGIDS PKVEVLRGIS LSIHPGEFVA IVGASGSGKS TLMNILGCLD
RPTSGSYRFA GRDVAELDSD ELAWLRREAF GFVFQGYHLI PSGSAQENVE MPAIYAGTPP
AERHARALAL LDRLGLASRT GNRPHQLSGG QQQRVSIARA LMNGGHIILA DEPTGALDSH
SGAEVMALLD ELASQGHVII LITHDREVAA RAQRIIEIRD GQMVSDSAAS QPSPAQPEQL
QANDLRQRLD RGAILKGAWK GEMIEALQAA WRVMWINRFR TALTLLGIII GVASVVVMLA
VGEGSKRQVM AQMAAFGSNI LYLNGKRATA QEPGGIVTLD DVAAIGELPQ VLHVMPVIGG
QLMVRQGNSS QKFYVGGNNT WFPAIFNWPV VEGTFFSEAD EASGAAVAVI GQKVRSKMFG
EGSNPLGQYL LIGNVPFQVV GILAAKGASS GSEDSDERIV VPYSAASIRL FGSHDPEYVA
IAAIDSRRVN ETEAAIDRLL RQRHQGKHDF DLTNDAALIQ AEARTQNSLS LMLGAIAAIS
LLVGGIGVMN IMLMTVRERT REIGIRMATG ARQRDILRQF LTEAVMLSMV GGVTGIVIAL
LVGGGLLLAD IAVAFALPAI LGAFACAVIT GVVFGFMPAR KAARLDPVKA LTSE
