ID   MACB_AROAE              Reviewed;         641 AA.
AC   Q5P6D5;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Macrolide export ATP-binding/permease protein MacB {ECO:0000255|HAMAP-Rule:MF_01720};
DE            EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01720};
GN   Name=macB {ECO:0000255|HAMAP-Rule:MF_01720}; OrderedLocusNames=AZOSEA10010;
GN   ORFNames=ebA1848;
OS   Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Aromatoleum.
OX   NCBI_TaxID=76114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EbN1;
RX   PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA   Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA   Reinhardt R.;
RT   "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT   bacterium, strain EbN1.";
RL   Arch. Microbiol. 183:27-36(2005).
CC   -!- FUNCTION: Non-canonical ABC transporter that contains transmembrane
CC       domains (TMD), which form a pore in the inner membrane, and an ATP-
CC       binding domain (NBD), which is responsible for energy generation.
CC       Confers resistance against macrolides. {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01720}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Macrolide
CC       exporter (TC 3.A.1.122) family. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAI07126.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CR555306; CAI07126.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q5P6D5; -.
DR   SMR; Q5P6D5; -.
DR   STRING; 76114.ebA1848; -.
DR   EnsemblBacteria; CAI07126; CAI07126; ebA1848.
DR   KEGG; eba:ebA1848; -.
DR   eggNOG; COG0577; Bacteria.
DR   eggNOG; COG1136; Bacteria.
DR   HOGENOM; CLU_000604_78_2_4; -.
DR   OMA; VVILITH; -.
DR   Proteomes; UP000006552; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003838; ABC3_permease_dom.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR017911; MacB_ATP-bd.
DR   InterPro; IPR025857; MacB_PCD.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF02687; FtsX; 1.
DR   Pfam; PF12704; MacB_PCD; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51267; MACB; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Cell inner membrane; Cell membrane;
KW   Membrane; Nucleotide-binding; Reference proteome; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..641
FT                   /note="Macrolide export ATP-binding/permease protein MacB"
FT                   /id="PRO_0000269920"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        516..536
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        573..593
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        604..624
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   DOMAIN          4..242
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
SQ   SEQUENCE   641 AA;  67786 MW;  ECD6710C803537A6 CRC64;
     MPLIELSGVT KTFRNGELAV EVLHGIDLTI LPGEFVAIVG SSGSGKSTLM NILGCLDRPT
     SGSYRFMGRN VAAFDRDELA LLRRETFGFV FQSYHLIGGA SARENVEVPA VYSGMPPAER
     HARATGLLAS LGLGERIEHR PNQLSGGQQQ RVSIARALMN GGRVILADEP TGALDTKSGA
     EVMQLLNKLS ADGHTIILIT HEREVAEQAQ RIIEIRDGRI VADPGPRPRS GLEPDFAPHV
     DRTSPLSDIV EAARTALRAL RANIFRAALT LLGIVIGVAA VIAMLAIGDG AKQDVVDRIS
     SMGTNLLTVR PGAPNQRGRD TTATLVLDDV RAIRDLPNVL AAVPEQSSTV TIRSGNADHR
     TSANATGADF TLARAWPIAR GTFFGAADER SYATVAVLGQ TVAKALFGDA DPVGEFVLVN
     SIMFQVLGVM GPQGATPWGT DQDDVIFVPY STGSLRLFGQ RHLRNATIAV EDVAAIDDTQ
     AAVHELLQAR HGGIEDFQIR NMASVIESVS ETQNTLTVLL GTVAAISLLV GGIGVMNIML
     VSVTERTREI GIRMATGARM KNILQQFLIE ALVVSALGGV IGVVVGLGAA AIIEAFDTPI
     VYSAPPVLLA FGCAFATGLV FGYLPARKAA RLDPVVALAS E