ID   MACB_BRADU              Reviewed;         653 AA.
AC   Q89NX6;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Macrolide export ATP-binding/permease protein MacB {ECO:0000255|HAMAP-Rule:MF_01720};
DE            EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01720};
GN   Name=macB {ECO:0000255|HAMAP-Rule:MF_01720}; OrderedLocusNames=bll3707;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Non-canonical ABC transporter that contains transmembrane
CC       domains (TMD), which form a pore in the inner membrane, and an ATP-
CC       binding domain (NBD), which is responsible for energy generation.
CC       Confers resistance against macrolides. {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01720}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Macrolide
CC       exporter (TC 3.A.1.122) family. {ECO:0000255|HAMAP-Rule:MF_01720}.
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DR   EMBL; BA000040; BAC48972.1; -; Genomic_DNA.
DR   RefSeq; NP_770347.1; NC_004463.1.
DR   RefSeq; WP_011086488.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89NX6; -.
DR   SMR; Q89NX6; -.
DR   STRING; 224911.27351967; -.
DR   EnsemblBacteria; BAC48972; BAC48972; BAC48972.
DR   GeneID; 64023437; -.
DR   KEGG; bja:bll3707; -.
DR   PATRIC; fig|224911.44.peg.3374; -.
DR   eggNOG; COG0577; Bacteria.
DR   eggNOG; COG1136; Bacteria.
DR   HOGENOM; CLU_000604_78_2_5; -.
DR   InParanoid; Q89NX6; -.
DR   OMA; NEIGVRM; -.
DR   PhylomeDB; Q89NX6; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003838; ABC3_permease_dom.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR017911; MacB_ATP-bd.
DR   InterPro; IPR025857; MacB_PCD.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF02687; FtsX; 1.
DR   Pfam; PF12704; MacB_PCD; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51267; MACB; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Cell inner membrane; Cell membrane;
KW   Membrane; Nucleotide-binding; Reference proteome; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..653
FT                   /note="Macrolide export ATP-binding/permease protein MacB"
FT                   /id="PRO_0000269925"
FT   TRANSMEM        277..297
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        526..546
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        587..607
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        617..637
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   DOMAIN          6..244
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   BINDING         42..49
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
SQ   SEQUENCE   653 AA;  70187 MW;  969E530D6D385296 CRC64;
     MTEPILALSH ICREFMTGDT KVAALADVTL DIDRGELVAI IGSSGSGKST LLNILGCLDH
     ATSGSYRVAG EDVAALDADA LAALRREHFG FIFQRYHLLS ELPALENVEI PAVYAGDTAS
     ERQARAERLL ARLGMAERRG HRPNQLSGGQ QQRVSIARAL MNGAEVVLAD EPTGALDRRS
     GEEVLKILVE LHREGKTIII VTHDPEVAKR ANRVIELRDG LVVSDKRTDA AVATAAASLP
     ADKPDTRSSR WSGLAFRFRE TLRMALLSMA AHRLRSFLTM LGIIIGIAAV SSVVALGNAS
     QNKVLSDISN LGTNTIEVFP GKDFGDARAG KIKTLVLDDA RALDRQTFIA GVTPTVSTST
     TVRYRDRESN VLVNGVDRSY FQVKGTKIAA GALFDAVAVR NIERQAVIDD NTRRTFFSDD
     QNAGIGRVIW VGKVPCRVVG VIAQQQGGFG SNQNLSVYLP YTTVQAQFTG DRSLRSVLLR
     VSDEVSTDLA QEAVTTLLTR RHSTKDFVIL NTDDIRRTIT STTQTLAFLV AAIAVISLVV
     GGIGVMNIML VSVSERIGEI GVRMAVGARR EDILQQFLVE ATLISSLGGI AGILIAVALG
     ALLNLLLPGF QVSYSTFSIG AAFLTSTAIG IFFGYFPARR AASFDPVVAL SRE