MACB_BRUME
ID MACB_BRUME Reviewed; 647 AA.
AC Q8YIT2; Q8YIT1;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Macrolide export ATP-binding/permease protein MacB {ECO:0000255|HAMAP-Rule:MF_01720};
DE EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01720};
GN Name=macB {ECO:0000255|HAMAP-Rule:MF_01720};
GN OrderedLocusNames=BMEI0360/BMEI0361;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC -!- FUNCTION: Non-canonical ABC transporter that contains transmembrane
CC domains (TMD), which form a pore in the inner membrane, and an ATP-
CC binding domain (NBD), which is responsible for energy generation.
CC Confers resistance against macrolides. {ECO:0000255|HAMAP-
CC Rule:MF_01720}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01720}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01720}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Macrolide
CC exporter (TC 3.A.1.122) family. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL51541.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
CC Sequence=AAL51542.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
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DR EMBL; AE008917; AAL51541.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE008917; AAL51542.1; ALT_SEQ; Genomic_DNA.
DR PIR; AB3297; AB3297.
DR PIR; AC3297; AC3297.
DR AlphaFoldDB; Q8YIT2; -.
DR SMR; Q8YIT2; -.
DR STRING; 224914.BMEI0360; -.
DR EnsemblBacteria; AAL51541; AAL51541; BMEI0360.
DR EnsemblBacteria; AAL51542; AAL51542; BMEI0361.
DR KEGG; bme:BMEI0360; -.
DR KEGG; bme:BMEI0361; -.
DR eggNOG; COG1136; Bacteria.
DR eggNOG; COG4591; Bacteria.
DR OMA; VVILITH; -.
DR Proteomes; UP000000419; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003838; ABC3_permease_dom.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR017911; MacB_ATP-bd.
DR InterPro; IPR025857; MacB_PCD.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF02687; FtsX; 1.
DR Pfam; PF12704; MacB_PCD; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51267; MACB; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; ATP-binding; Cell inner membrane; Cell membrane;
KW Membrane; Nucleotide-binding; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..647
FT /note="Macrolide export ATP-binding/permease protein MacB"
FT /id="PRO_0000269927"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 573..593
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT TRANSMEM 610..630
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT DOMAIN 7..245
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT BINDING 43..50
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
SQ SEQUENCE 647 AA; 69107 MW; 69E391F204C1588E CRC64;
MAGAPLIRLE DICKTFHNGD LAVEVLHGIT LDIRAGEFVA IMGASGSGKS TLMNILGCLD
TPTGGRYLLD GEDVSTLNAD ELATLRRRTF GFVFQSYNLI PTSTAQENVE VPAIYAGTPA
AERRKRAAAL LNALKLGDRL DHRPSQLSGG QQQRVSIARA LMNGGRIILA DEPTGALDSQ
SGEDVMELLR SMHQQGHTVI VITHAREVAE RADRLIEIRD GQILSDTTKR DIHTPEATLQ
PHEEIAGNGA HIADISEAVK MALRALRANI FRTVLTLLGI IIGVSSVVTM LAIGTGAQNT
ILDRINAMGT DLILVRPAMA GFRGSGSIAT LVPQDADAIL ELPNVKSAVP EVTGTVTLRR
GNVDYQSQAN GTVPAFSLSE IVESRQRQLH HPERYRYLRP CGWLGTTVVK TLFPDGSNPV
GDYILIQKIP FQIIGTLEPK GAGFGGSDQD DVVVVPLSTG NLRLFGQKYV RSITVQVKDS
SLIDTTQNQI QSLLDQRHKK RDTMITNMSS VREDAAAMGK TMTVFLGSVA AISLLVGGIG
VMNIMLVSVT ERTREIGVRM ATGARRRDIL LQFIIEALSV SAIGGAIGVI LGLGAAALAS
WAGLSVGYSF GPVLLAFACA FATGLIFGFL PARKASRLLP AVALSSE
