ID   MACB_NITEU              Reviewed;         659 AA.
AC   Q82VK1;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Macrolide export ATP-binding/permease protein MacB {ECO:0000255|HAMAP-Rule:MF_01720};
DE            EC=7.6.2.- {ECO:0000255|HAMAP-Rule:MF_01720};
GN   Name=macB {ECO:0000255|HAMAP-Rule:MF_01720}; OrderedLocusNames=NE1081;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
CC   -!- FUNCTION: Non-canonical ABC transporter that contains transmembrane
CC       domains (TMD), which form a pore in the inner membrane, and an ATP-
CC       binding domain (NBD), which is responsible for energy generation.
CC       Confers resistance against macrolides. {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01720}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01720}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01720}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Macrolide
CC       exporter (TC 3.A.1.122) family. {ECO:0000255|HAMAP-Rule:MF_01720}.
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DR   EMBL; AL954747; CAD84992.1; -; Genomic_DNA.
DR   RefSeq; WP_011111680.1; NC_004757.1.
DR   AlphaFoldDB; Q82VK1; -.
DR   SMR; Q82VK1; -.
DR   STRING; 228410.NE1081; -.
DR   EnsemblBacteria; CAD84992; CAD84992; NE1081.
DR   KEGG; neu:NE1081; -.
DR   eggNOG; COG0577; Bacteria.
DR   eggNOG; COG1136; Bacteria.
DR   HOGENOM; CLU_000604_78_2_4; -.
DR   OMA; VVILITH; -.
DR   OrthoDB; 1181903at2; -.
DR   PhylomeDB; Q82VK1; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd03255; ABC_MJ0796_LolCDE_FtsE; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003838; ABC3_permease_dom.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR017911; MacB_ATP-bd.
DR   InterPro; IPR025857; MacB_PCD.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF02687; FtsX; 1.
DR   Pfam; PF12704; MacB_PCD; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51267; MACB; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Cell inner membrane; Cell membrane;
KW   Membrane; Nucleotide-binding; Reference proteome; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..659
FT                   /note="Macrolide export ATP-binding/permease protein MacB"
FT                   /id="PRO_0000269952"
FT   TRANSMEM        287..307
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        538..558
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        594..614
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   TRANSMEM        619..639
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   DOMAIN          10..249
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
FT   BINDING         47..54
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01720"
SQ   SEQUENCE   659 AA;  72075 MW;  5A591DE060CA4E0D CRC64;
     MVSTDLPPLI ELRGIRKRYG GGDKPEVEVL HGIDLDIRAG EFIAIVGSSG SGKSTLMHLL
     GCLDRPSSGS YRFAGEDVST FGSDELAWLR RKAFGFVFQG YHLIPTESAR ENVEIPAIYA
     GLPPGERMQR AADLLGRLGL SDKLNNRPNQ LSGGQQQRVS IARALMNGGH IILADEPTGA
     LDSRSGAEVM ELLRELAGAG HTVILITHDR DVAAQAQRVV EIRDGRIVAD SVTDRQPSEQ
     PLLHHAGLSS LEMTQAHEDT GTPFWQGLHE TIRAAWRVMW IHRVRTSLTL LGIVIGVASV
     IVMLAIGEGT KQRVIDQMGS MGTTIMYMSS DVPSTGGPVG VITEEDLDEV ARLPEISRVM
     PVIGDPILVR HQNVDKQIYV FSSPYIMPMV HHWRVAQGRF FTETEDRELA PVVVLGHKIY
     RSFFPHLSNP VGQYLLIGTS PFEVIGVMAE RGAESGSQNY DDMVFIPYRA GRARVYQAQE
     QPDYIVMEAA SMDQVQEAEE AIRALLLERH GREDFRIGNA AARLKTQLET RDTMTRMLGL
     VAAVSLLVGG IGVMNVMLMT VRERTREIGI RMATGAREYD ILSQFLIEAM LVTITGGTVG
     VILGLTVGAL LVFWEVPVVF SFGVMIGAFA CAVITGLIFG YMPARTAARL DPVVALSSE