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MACC_PENTR
ID   MACC_PENTR              Reviewed;         536 AA.
AC   A0A2P1DP94;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2018, sequence version 1.
DT   03-AUG-2022, entry version 8.
DE   RecName: Full=Cytochrome P450 monooxygenase macC {ECO:0000303|PubMed:28926261};
DE            EC=1.-.-.- {ECO:0000250|UniProtKB:G3Y416};
DE   AltName: Full=Macrophorins biosynthesis cluster protein C {ECO:0000303|PubMed:28926261};
GN   Name=macC {ECO:0000303|PubMed:28926261};
OS   Penicillium terrestre.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=374132;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=LM2;
RX   PubMed=28926261; DOI=10.1021/acs.orglett.7b02653;
RA   Tang M.C., Cui X., He X., Ding Z., Zhu T., Tang Y., Li D.;
RT   "Late-stage terpene cyclization by an integral membrane cyclase in the
RT   biosynthesis of isoprenoid epoxycyclohexenone natural products.";
RL   Org. Lett. 19:5376-5379(2017).
CC   -!- FUNCTION: Cytochrome P450 monooxygenase; part of the gene cluster that
CC       mediates the biosynthesis of macrophorins, isoprenoid
CC       epoxycyclohexenones containing cyclized drimane moieties
CC       (PubMed:28926261). The first step of the pathway is the synthesis of 6-
CC       methylsalicylic acid (6-MSA) by the polyketide synthase macA
CC       (PubMed:28926261). 6-MSA is then converted to m-cresol by the
CC       decarboxylase macB (By similarity). The cytochrome P450 monooxygenase
CC       macC then catalyzes the oxidation of m-cresol to toluquinol (By
CC       similarity). Epoxidation of toluquinol is then performed by the short
CC       chain dehydrogenase macD, with the help of macE, and a further
CC       prenylation by macG leads to 7-deacetoxyyanuthone A (By similarity).
CC       The next step is the hydroxylation of C-22 of 7-deacetoxyyanuthone A by
CC       the cytochrome P450 monooxygenase macH to yield 22-deacetylyanuthone A
CC       (By similarity). O-Mevalon transferase macI then attaches mevalon to
CC       the hydroxyl group of 22-deacetylyanuthone A to produce yanuthone E (By
CC       similarity). The terpene cyclase macJ catalyzes the cyclization of 22-
CC       deacetylyanuthone A to macrophorin A (PubMed:28926261). MacJ is also
CC       able to catalyze cyclization of yanuthone E and 7-deacetoxyyanuthone A
CC       to their corresponding macrophorins (PubMed:28926261). The macJ
CC       products can be further modified by macH and macJ, as well as by the
CC       FAD-dependent monooxygenase macF, to produce additional macrophorins,
CC       including 4'-oxomacrophorin A, 4'-oxomacrophorin D and 4'-
CC       oxomacrophorin E (PubMed:28926261). {ECO:0000250|UniProtKB:G3Y416,
CC       ECO:0000269|PubMed:28926261}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:28926261}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- MISCELLANEOUS: The macrophorins cluster contains a single gene
CC       insertion (encoding for the terpene cyclase macJ) compared with the
CC       yanuthone cluster that produces the linear compound yanuthone.
CC       {ECO:0000269|PubMed:28926261}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; MF989996; AVK70097.1; -; Genomic_DNA.
DR   EMBL; MH388470; QBC75451.1; -; Genomic_DNA.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..536
FT                   /note="Cytochrome P450 monooxygenase macC"
FT                   /id="PRO_0000454086"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         448
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
SQ   SEQUENCE   536 AA;  61412 MW;  C980E6537D1E4DA4 CRC64;
     MALLYITTAA LALLLLFLRA VFKSWRIQRK LPPGPPGLPL IGNIHQIPAV RAHQKFTEWA
     KVYGGLYTFR IGPATAAVIT DRGLVKELLD KRSALYSSRP VSYVGQNLIT GGDHLLLMDN
     NEMWRLFRKT VHQHFKASMC EKEHVKLLEA EHTQMMRDFL LYPEKHMLHT KRTTNSIIMS
     LLYGIRTPSW DTPHMRELYE IMEQWSKVME TGATPPVDIF PWLRWIPQRW LGNWVDRSVE
     VGSGMKALYG SFRRRAIEAR REAEQSSQSR ARTFIDHVLD LQEKANLTDN QVDFLGGVMM
     EGGSDTGSTM LLVMIQALVR YPEVQERARA ELDAVCGEGR SPTWADFSRL PYINMIVKET
     MRWRPVTPLS FPHALNQDDW VNGYLLPKGT TVFLNVWGLH HDESIFPNPE RFDPSHYEGR
     HNLASDYAAS PDYMQRDHFI YGAGRRLCPG IHLSERSMFI GAAKLLWCFQ FEPEMDESGR
     PVAIDTDPIT GYTEGFLVCP QAYKCKVSPR STARAETIMR EFAQAESEVL CQYATP
 
 
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