MACE_PENTR
ID MACE_PENTR Reviewed; 260 AA.
AC A0A2P1DP82;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2018, sequence version 1.
DT 03-AUG-2022, entry version 6.
DE RecName: Full=Oxidoreductase macE {ECO:0000303|PubMed:28926261};
DE EC=1.-.-.- {ECO:0000250|UniProtKB:G3Y423};
DE AltName: Full=Macrophorins biosynthesis cluster protein E {ECO:0000303|PubMed:28926261};
GN Name=macE {ECO:0000303|PubMed:28926261};
OS Penicillium terrestre.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=374132;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC STRAIN=LM2;
RX PubMed=28926261; DOI=10.1021/acs.orglett.7b02653;
RA Tang M.C., Cui X., He X., Ding Z., Zhu T., Tang Y., Li D.;
RT "Late-stage terpene cyclization by an integral membrane cyclase in the
RT biosynthesis of isoprenoid epoxycyclohexenone natural products.";
RL Org. Lett. 19:5376-5379(2017).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of macrophorins, isoprenoid epoxycyclohexenones containing
CC cyclized drimane moieties (PubMed:28926261). The first step of the
CC pathway is the synthesis of 6-methylsalicylic acid (6-MSA) by the
CC polyketide synthase macA (PubMed:28926261). 6-MSA is then converted to
CC m-cresol by the decarboxylase macB (By similarity). The cytochrome P450
CC monooxygenase macC then catalyzes the oxidation of m-cresol to
CC toluquinol (By similarity). Epoxidation of toluquinol is then performed
CC by the short chain dehydrogenase macD, with the help of macE, and a
CC further prenylation by macG leads to 7-deacetoxyyanuthone A (By
CC similarity). The next step is the hydroxylation of C-22 of 7-
CC deacetoxyyanuthone A by the cytochrome P450 monooxygenase macH to yield
CC 22-deacetylyanuthone A (By similarity). O-Mevalon transferase macI then
CC attaches mevalon to the hydroxyl group of 22-deacetylyanuthone A to
CC produce yanuthone E (By similarity). The terpene cyclase macJ catalyzes
CC the cyclization of 22-deacetylyanuthone A to macrophorin A
CC (PubMed:28926261). MacJ is also able to catalyze cyclization of
CC yanuthone E and 7-deacetoxyyanuthone A to their corresponding
CC macrophorins (PubMed:28926261). The macJ products can be further
CC modified by macH and macJ, as well as by the FAD-dependent
CC monooxygenase macF, to produce additional macrophorins, including 4'-
CC oxomacrophorin A, 4'-oxomacrophorin D and 4'-oxomacrophorin E
CC (PubMed:28926261). {ECO:0000250|UniProtKB:G3Y423,
CC ECO:0000269|PubMed:28926261}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:28926261}.
CC -!- MISCELLANEOUS: The macrophorins cluster contains a single gene
CC insertion (encoding for the terpene cyclase macJ) compared with the
CC yanuthone cluster that produces the linear compound yanuthone.
CC {ECO:0000269|PubMed:28926261}.
CC -!- SIMILARITY: Belongs to the oxidoreductase OpS7 family. {ECO:0000305}.
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DR EMBL; MF990003; AVK70104.1; -; Genomic_DNA.
DR EMBL; MH388470; QBC75444.1; -; Genomic_DNA.
DR UniPathway; UPA00213; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 3: Inferred from homology;
KW Oxidoreductase.
FT CHAIN 1..260
FT /note="Oxidoreductase macE"
FT /id="PRO_0000454089"
SQ SEQUENCE 260 AA; 29586 MW; 5AB3A043B86020D4 CRC64;
MAPCKPRTFT AGQDPLTKFD GAISVRNLPR PPDRLFLFHG IMRPSRGIYA KLIATGQKPP
THFHPSQWEF FRVLRGNLTI DFNGRAIHRT ASDGELAVPP YTHHVIYGTP GTEMNEVEFV
VSASDPAAEE QGATVMDQPF FENWYGYQED VFQRGEKLDF IQVLSMFDAG GTYLSPPWWV
PFRSWVGLFL GIVVGRWIGG LLGYAPFYPE WTTDWEAACE TMQQSWFQRR FADPQAQERA
REKFRGQLEQ EASAKGGKSE