MACF_PENTR
ID MACF_PENTR Reviewed; 519 AA.
AC A0A2P1DP98;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-MAY-2018, sequence version 1.
DT 03-AUG-2022, entry version 10.
DE RecName: Full=FAD-dependent monooxygenase macF {ECO:0000303|PubMed:28926261};
DE EC=1.-.-.- {ECO:0000250|UniProtKB:G3Y424};
DE AltName: Full=Macrophorins biosynthesis cluster protein F {ECO:0000303|PubMed:28926261};
DE Flags: Precursor;
GN Name=macF {ECO:0000303|PubMed:28926261};
OS Penicillium terrestre.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=374132;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC STRAIN=LM2;
RX PubMed=28926261; DOI=10.1021/acs.orglett.7b02653;
RA Tang M.C., Cui X., He X., Ding Z., Zhu T., Tang Y., Li D.;
RT "Late-stage terpene cyclization by an integral membrane cyclase in the
RT biosynthesis of isoprenoid epoxycyclohexenone natural products.";
RL Org. Lett. 19:5376-5379(2017).
CC -!- FUNCTION: FAD-dependent monooxygenase; part of the gene cluster that
CC mediates the biosynthesis of macrophorins, isoprenoid
CC epoxycyclohexenones containing cyclized drimane moieties
CC (PubMed:28926261). The first step of the pathway is the synthesis of 6-
CC methylsalicylic acid (6-MSA) by the polyketide synthase macA
CC (PubMed:28926261). 6-MSA is then converted to m-cresol by the
CC decarboxylase macB (By similarity). The cytochrome P450 monooxygenase
CC macC then catalyzes the oxidation of m-cresol to toluquinol (By
CC similarity). Epoxidation of toluquinol is then performed by the short
CC chain dehydrogenase macD, with the help of macE, and a further
CC prenylation by macG leads to 7-deacetoxyyanuthone A (By similarity).
CC The next step is the hydroxylation of C-22 of 7-deacetoxyyanuthone A by
CC the cytochrome P450 monooxygenase macH to yield 22-deacetylyanuthone A
CC (By similarity). O-Mevalon transferase macI then attaches mevalon to
CC the hydroxyl group of 22-deacetylyanuthone A to produce yanuthone E (By
CC similarity). The terpene cyclase macJ catalyzes the cyclization of 22-
CC deacetylyanuthone A to macrophorin A (PubMed:28926261). MacJ is also
CC able to catalyze cyclization of yanuthone E and 7-deacetoxyyanuthone A
CC to their corresponding macrophorins (PubMed:28926261). The macJ
CC products can be further modified by macH and macJ, as well as by the
CC FAD-dependent monooxygenase macF, to produce additional macrophorins,
CC including 4'-oxomacrophorin A, 4'-oxomacrophorin D and 4'-
CC oxomacrophorin E (PubMed:28926261). {ECO:0000250|UniProtKB:G3Y424,
CC ECO:0000269|PubMed:28926261}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:28926261}.
CC -!- MISCELLANEOUS: The macrophorins cluster contains a single gene
CC insertion (encoding for the terpene cyclase macJ) compared with the
CC yanuthone cluster that produces the linear compound yanuthone.
CC {ECO:0000269|PubMed:28926261}.
CC -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; MF990005; AVK70106.1; -; Genomic_DNA.
DR EMBL; MH388470; QBC75442.1; -; Genomic_DNA.
DR UniPathway; UPA00213; -.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.465.10; -; 1.
DR InterPro; IPR016166; FAD-bd_PCMH.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR006094; Oxid_FAD_bind_N.
DR Pfam; PF01565; FAD_binding_4; 1.
DR SUPFAM; SSF56176; SSF56176; 1.
DR PROSITE; PS51387; FAD_PCMH; 1.
PE 3: Inferred from homology;
KW FAD; Flavoprotein; Oxidoreductase; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..519
FT /note="FAD-dependent monooxygenase macF"
FT /id="PRO_5015136411"
FT DOMAIN 88..262
FT /note="FAD-binding PCMH-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00718"
FT MOD_RES 125
FT /note="Pros-8alpha-FAD histidine"
FT /evidence="ECO:0000250|UniProtKB:P08159"
SQ SEQUENCE 519 AA; 55144 MW; 3975E83101269FC5 CRC64;
MTKMTSIIGI LMGVLTTATA ASIPTGSSAA AAAALGSLGV SPPSGNVLIG NAGYTCSLLN
RVLSKNETFT VTSPYYDVLI DEAWSENCRL NASCIVTPES AEEVSRLLQI LSILETRFAI
RSGGHNTNPG FSSIGSDGVL IALEKLDSIS LSADRGTVTV GPGNKWESVY KYLQPYNLTA
LGGREAVVGV GGYILGETGG LSTFYNTHGL AIDSVTRFQV VLPNGTIVDA TPTEHADLYK
GLKGGLNNFG IVTEYDLTTN TGVDIYYEIK TYTTANTPAV LAAYATYLLD ADINSNVEIQ
INPSYTLVFY GYLGHVSAPT DFDPFSDIPV ASTMYPPTNG SLTELLLSIG STGLTSEGVS
YSGTFSFKVT GSTFLQDTYS TYLEAAASLP TGAVLSYVPQ GVIPNLVTQG KSQNGGNLLG
LDATPQVWAN IFVQFPATLS QSEVAGSVDS LLANLISSAK SEDLFLPYIF VNDAGAKQKP
LQSFGEKNIK YIDTVAKRYD PKRIMQRLQN QAYFVLEEL