ID   MAD1_ARATH              Reviewed;         726 AA.
AC   Q9LTY1;
DT   26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Mitotic spindle checkpoint protein MAD1 {ECO:0000303|PubMed:22457071};
DE   AltName: Full=Mitotic arrest deficient protein 1;
GN   Name=MAD1 {ECO:0000303|PubMed:22457071};
GN   OrderedLocusNames=At5g49880 {ECO:0000312|Araport:AT5G49880};
GN   ORFNames=K9P8.2 {ECO:0000312|EMBL:BAA97009.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, INTERACTION WITH NUA AND MAD2, AND SUBCELLULAR LOCATION.
RX   PubMed=22457071; DOI=10.1007/s11103-012-9903-4;
RA   Ding D., Muthuswamy S., Meier I.;
RT   "Functional interaction between the Arabidopsis orthologs of spindle
RT   assembly checkpoint proteins MAD1 and MAD2 and the nucleoporin NUA.";
RL   Plant Mol. Biol. 79:203-216(2012).
CC   -!- FUNCTION: Required for the execution of the mitotic checkpoint which
CC       monitors the process of kinetochore-spindle attachment and delays the
CC       onset of anaphase when this process is not complete. It inhibits the
CC       activity of the anaphase promoting complex by sequestering CDC20 until
CC       all chromosomes are aligned at the metaphase plate. Required for
CC       anchoring MAD2 to the nuclear envelope. {ECO:0000269|PubMed:22457071}.
CC   -!- SUBUNIT: Homodimer. Part of the mitotic checkpoint complex (MCC).
CC       Interacts with MAD2 and NUA. {ECO:0000269|PubMed:22457071}.
CC   -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:22457071}.
CC       Note=The nucleus envelope association requires the presence of NUA.
CC       {ECO:0000269|PubMed:22457071}.
CC   -!- SIMILARITY: Belongs to the MAD1 family. {ECO:0000305}.
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DR   EMBL; AB024032; BAA97009.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95867.1; -; Genomic_DNA.
DR   RefSeq; NP_199799.1; NM_124366.4.
DR   AlphaFoldDB; Q9LTY1; -.
DR   SMR; Q9LTY1; -.
DR   BioGRID; 20297; 5.
DR   STRING; 3702.AT5G49880.1; -.
DR   iPTMnet; Q9LTY1; -.
DR   PaxDb; Q9LTY1; -.
DR   PRIDE; Q9LTY1; -.
DR   ProteomicsDB; 238646; -.
DR   EnsemblPlants; AT5G49880.1; AT5G49880.1; AT5G49880.
DR   GeneID; 835051; -.
DR   Gramene; AT5G49880.1; AT5G49880.1; AT5G49880.
DR   KEGG; ath:AT5G49880; -.
DR   Araport; AT5G49880; -.
DR   TAIR; locus:2158804; AT5G49880.
DR   eggNOG; KOG4593; Eukaryota.
DR   HOGENOM; CLU_023152_0_0_1; -.
DR   InParanoid; Q9LTY1; -.
DR   OMA; YMLLGYR; -.
DR   OrthoDB; 602490at2759; -.
DR   PhylomeDB; Q9LTY1; -.
DR   PRO; PR:Q9LTY1; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LTY1; baseline and differential.
DR   Genevisible; Q9LTY1; AT.
DR   GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR   GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR   GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   InterPro; IPR008672; Mad1.
DR   PANTHER; PTHR23168; PTHR23168; 1.
DR   Pfam; PF05557; MAD; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Coiled coil; Mitosis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..726
FT                   /note="Mitotic spindle checkpoint protein MAD1"
FT                   /id="PRO_0000431096"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          68..246
FT                   /evidence="ECO:0000255"
FT   COILED          272..625
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   726 AA;  82433 MW;  03F7EF063968F176 CRC64;
     MILRTPQPKR LRSDAGESPF PTGATGSGNQ LIIYEDSPLP APAPLQTSHD HSADQHLCTY
     QCRQMVKADV LDALSTAEKQ VEESKTKLQT LNANFTEADA ERKHFRDKFL YSEQELAAAK
     AREKMLQEQL LMEINNSQER YTKELQSCHE LEVKLQNEMN LRKKAESSAA TAEEKAKLLE
     DKLTQLSGSV DREKKRLNND IAQLGKEAKL SVARIGADLE RMQCRAQNAE TESNLLRSQL
     EHLKLIFDEC LQEKTEVDKK LSSFTSEAAS SSDNSVLVKH LQEELKRYEA EVREARKLKS
     RHLDAELLNV NLLEEQSRRE RAESELSKFH DLQLSMEKLE NELSSWKSLL NDIPGVSCPD
     DIVMRFSVLQ NEVVQSTMKI GEASTRIKQL EETLEAIQLG RQNAVSEAAL AKEKSEALKT
     DVKRIEVMLT LVTEEKEQLK AVVNELRKSN SEGSVSGAAD GALIQGFESS LAKKENYIKD
     LEQDLNQLKD VNNRQRTEIE LLNEKLVDEA RRNKSLERDS DRLRSEISLL ESKLGHGDYS
     AANTRVLRMV NTLGVENEAK QTIEALQAEL QKTKERLQAV EELKSQSGDA GKLVDSHITG
     KIAQLKEQNA TLEKREERYK TVFADRISVF RRACCELFGY KIVMDEHQRP NGIPVTRFTL
     QSIYAQSDDE KLEFEYESGN TSILNNEYAS QGDIAKQIEI FIRKFNSIPA FTANLTMESF
     NRRTLY