ID   MAD1_ASHGO              Reviewed;         659 AA.
AC   Q754Z1;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Spindle assembly checkpoint component MAD1;
DE   AltName: Full=Mitotic arrest deficient protein 1;
GN   Name=MAD1; OrderedLocusNames=AFL065C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 136.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Central component of the spindle assembly checkpoint.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MAD1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016819; AAS53307.2; -; Genomic_DNA.
DR   RefSeq; NP_985483.2; NM_210837.2.
DR   AlphaFoldDB; Q754Z1; -.
DR   SMR; Q754Z1; -.
DR   STRING; 33169.AAS53307; -.
DR   EnsemblFungi; AAS53307; AAS53307; AGOS_AFL065C.
DR   GeneID; 4621713; -.
DR   KEGG; ago:AGOS_AFL065C; -.
DR   eggNOG; KOG4593; Eukaryota.
DR   HOGENOM; CLU_026595_0_0_1; -.
DR   InParanoid; Q754Z1; -.
DR   OMA; YMLLGYR; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR   GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR   GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR   GO; GO:0044877; F:protein-containing complex binding; IEA:EnsemblFungi.
DR   GO; GO:0090268; P:activation of mitotic cell cycle spindle assembly checkpoint; IEA:EnsemblFungi.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0044774; P:mitotic DNA integrity checkpoint signaling; IEA:EnsemblFungi.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   GO; GO:1901925; P:negative regulation of protein import into nucleus during spindle assembly checkpoint; IEA:EnsemblFungi.
DR   GO; GO:0006913; P:nucleocytoplasmic transport; IEA:EnsemblFungi.
DR   InterPro; IPR008672; Mad1.
DR   PANTHER; PTHR23168; PTHR23168; 1.
DR   Pfam; PF05557; MAD; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Coiled coil; Mitosis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..659
FT                   /note="Spindle assembly checkpoint component MAD1"
FT                   /id="PRO_0000213791"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          23..531
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   659 AA;  75421 MW;  3E0F7EE664857D4E CRC64;
     MSQTGDSSSF VEEPASPEAR QRELERQVLG LEYRLRTEHS EAEMARLALE RELAGMQGKY
     KQCMGELEAA LADTRYLYEQ NAALEARARA ADEDAGGAAA AERAAAAEER ARAAEARARE
     LEEALQAARG EAAAAGRAAA GAAAGAAAAE QSGLLRKYEA EIERQMGELR ALAERLAERE
     DEVAGLKAQR VLQAHRSYSA EEVEELGVLR RTLQEQLALS KELERANLEQ ANELKRLRQR
     SESQQFLQLE NSKLRARLEK TDALRQQLDD LQLENVQLQE KLTKWEVYQA DGSSPENVLH
     EMALAKQERL ALVEQVGKLQ LDLNNMKILN DELALERNQL LDLNKKYESS ILNLKKLNYE
     IEQQKLLSFE ECKLLRQQLD DLSEVDKDHT PDTRDRKKIQ TLVDTYKNQT EDLTNELKKL
     NEQLLERSDL DGNPRKRRKA SDDLAFNYSQ RLNELQLRNK DLERRLANSD ENASLLEEKL
     RKLQSLNEKK IRILQLRNNP LLKDQFVKQK QLTLLKQENL ELAQQLDAQQ GLEAVPRSVY
     ERQQYDIQLL EDELFSINKR TTRLKEMFNR KSLEFIDAVN SLLGFKLEFQ VGGKVKMIPC
     FKTDRYLIAD LQTNTLKSNL DKVMPEWDAM LAEYVGKRQQ LPCFLAKIML LLAESSSSQ