ID   MAD1_KLULA              Reviewed;         648 AA.
AC   Q6CMM2;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Spindle assembly checkpoint component MAD1;
DE   AltName: Full=Mitotic arrest deficient protein 1;
GN   Name=MAD1; OrderedLocusNames=KLLA0E19239g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Central component of the spindle assembly checkpoint.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MAD1 family. {ECO:0000305}.
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DR   EMBL; CR382125; CAG99904.1; -; Genomic_DNA.
DR   RefSeq; XP_454817.1; XM_454817.1.
DR   AlphaFoldDB; Q6CMM2; -.
DR   SMR; Q6CMM2; -.
DR   STRING; 28985.XP_454817.1; -.
DR   EnsemblFungi; CAG99904; CAG99904; KLLA0_E19141g.
DR   GeneID; 2893955; -.
DR   KEGG; kla:KLLA0_E19141g; -.
DR   eggNOG; KOG4593; Eukaryota.
DR   HOGENOM; CLU_026595_0_0_1; -.
DR   InParanoid; Q6CMM2; -.
DR   OMA; HHNYSTE; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0000776; C:kinetochore; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044877; F:protein-containing complex binding; IEA:EnsemblFungi.
DR   GO; GO:0090268; P:activation of mitotic cell cycle spindle assembly checkpoint; IEA:EnsemblFungi.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0044774; P:mitotic DNA integrity checkpoint signaling; IEA:EnsemblFungi.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IEA:EnsemblFungi.
DR   GO; GO:1901925; P:negative regulation of protein import into nucleus during spindle assembly checkpoint; IEA:EnsemblFungi.
DR   GO; GO:0006913; P:nucleocytoplasmic transport; IEA:EnsemblFungi.
DR   InterPro; IPR008672; Mad1.
DR   PANTHER; PTHR23168; PTHR23168; 1.
DR   Pfam; PF05557; MAD; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Coiled coil; Mitosis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..648
FT                   /note="Spindle assembly checkpoint component MAD1"
FT                   /id="PRO_0000213795"
FT   COILED          19..283
FT                   /evidence="ECO:0000255"
FT   COILED          322..483
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   648 AA;  76063 MW;  32824FD978EA0979 CRC64;
     MSLTPQIKEK PLESLDADQL RRRVLTLQYK YTTLQNEYEI SKLSSERETY SLQNKYDKSM
     NELENALKDT KILYEENIKL KEELKSKDAL PKEDEKIITT LRTQVVSLKN EVNLKEQRIV
     ELAHKLSSKQ NEVTNAIESM KLEIEGSGNI LHQHETQINK HVEEIRYLQQ ELKEKENLIS
     ELRSVQTSAS HRNNSTEELQ DLAVLNKTLK EQLNYAKELE DMNLKQATEL KKLRQNNDVQ
     SLLKKENELL QSKLDQLNSL QKKFESLEME NISLQEKITQ WGIVRLDGKF KNPNDVITEL
     NMLQRENTFL LDTNSKLQID FNQMSMLNDE MAIERNQLLD LNKDYETSIL NLKRLNHELE
     QQKLLSFEEC KLLRQQLEEF EEQDDKKKTP GDQVNASNII EGYKNQTEDL TNELKRLNEE
     LSKTDDKIQK RRKITNDLGI SYSQRLNELI LENKKLERLL SATKNHANIL EQKILDLTSL
     KEKKVRILQL RDNPLLKEHY IRKEKLALLE KENSDLLSGI ETDAIPRSVY DRIKHDMKLL
     EKEIFSANKK TTRLKEVFNK KSLEFIEAVN SLLGFKLEFL AHGKVKLVSC YQPNKYIIAD
     LQSNTLKSDL DKVIPEWETL FQLYVIEKGE LPAFLSQVKL RLWELSQP