MAD2_ARATH
ID MAD2_ARATH Reviewed; 209 AA.
AC Q9LU93; Q67YV5;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Mitotic spindle checkpoint protein MAD2 {ECO:0000303|PubMed:19710914};
DE AltName: Full=Mitotic arrest deficient protein 2;
GN Name=MAD2 {ECO:0000303|PubMed:19710914};
GN OrderedLocusNames=At3g25980 {ECO:0000312|Araport:AT3G25980};
GN ORFNames=MPE11.15 {ECO:0000312|EMBL:BAB01061.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH BUBR1 AND BUB3.1, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=19710914; DOI=10.1371/journal.pone.0006757;
RA Caillaud M.-C., Paganelli L., Lecomte P., Deslandes L., Quentin M.,
RA Pecrix Y., Le Bris M., Marfaing N., Abad P., Favery B.;
RT "Spindle assembly checkpoint protein dynamics reveal conserved and
RT unsuspected roles in plant cell division.";
RL PLoS ONE 4:E6757-E6757(2009).
RN [6]
RP INTERACTION WITH EIF4B3.
RX PubMed=20706207; DOI=10.1038/msb.2010.53;
RA Van Leene J., Hollunder J., Eeckhout D., Persiau G., Van De Slijke E.,
RA Stals H., Van Isterdael G., Verkest A., Neirynck S., Buffel Y., De Bodt S.,
RA Maere S., Laukens K., Pharazyn A., Ferreira P.C.G., Eloy N., Renne C.,
RA Meyer C., Faure J.-D., Steinbrenner J., Beynon J., Larkin J.C.,
RA Van de Peer Y., Hilson P., Kuiper M., De Veylder L., Van Onckelen H.,
RA Inze D., Witters E., De Jaeger G.;
RT "Targeted interactomics reveals a complex core cell cycle machinery in
RT Arabidopsis thaliana.";
RL Mol. Syst. Biol. 6:397-397(2010).
RN [7]
RP INTERACTION WITH CDC20-1; CDC20-2 AND CDC20-5.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
RN [8]
RP INTERACTION WITH MAD1, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=22457071; DOI=10.1007/s11103-012-9903-4;
RA Ding D., Muthuswamy S., Meier I.;
RT "Functional interaction between the Arabidopsis orthologs of spindle
RT assembly checkpoint proteins MAD1 and MAD2 and the nucleoporin NUA.";
RL Plant Mol. Biol. 79:203-216(2012).
CC -!- FUNCTION: Required for the execution of the mitotic checkpoint which
CC monitors the process of kinetochore-spindle attachment and delays the
CC onset of anaphase when this process is not complete. It inhibits the
CC activity of the anaphase promoting complex by sequestering CDC20 until
CC all chromosomes are aligned at the metaphase plate.
CC {ECO:0000269|PubMed:19710914}.
CC -!- SUBUNIT: Part of the mitotic checkpoint complex (MCC); interacts with
CC MAD1, CDC20-1, CDC20-2 and CDC20-5. Interacts with BUBR1 at
CC chromocenters and with BUB3.1. Interacts with EIF4B3 (PubMed:20706207).
CC {ECO:0000269|PubMed:19710914, ECO:0000269|PubMed:20706207,
CC ECO:0000269|PubMed:21687678}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19710914,
CC ECO:0000269|PubMed:22457071}. Nucleus envelope
CC {ECO:0000269|PubMed:22457071}. Chromosome
CC {ECO:0000269|PubMed:19710914}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:19710914}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:19710914}. Cytoplasm {ECO:0000269|PubMed:19710914,
CC ECO:0000269|PubMed:22457071}. Note=Cytoplasmic in interphase cells.
CC Accumulates onto both kinetochores and the spindle microtubules in cell
CC arrested in metaphase. Present in chromocenters. Associates with
CC unattached kinetochores upon spindle assembly checkpoint (SAC)
CC activation. The nucleus envelope association requires the presence of
CC NUA. {ECO:0000269|PubMed:22457071}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LU93-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in actively dividing tissues, early in
CC organ development, in young leaves, lateral root primordia and root
CC meristems. {ECO:0000269|PubMed:19710914}.
CC -!- INDUCTION: Cell cycle regulated expression with a distinct expression
CC peak at the G2/M boundary. {ECO:0000269|PubMed:19710914}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when grown under normal
CC growth conditions and stunted roots when grown in vitro in absence of
CC sucrose. {ECO:0000269|PubMed:22457071}.
CC -!- SIMILARITY: Belongs to the MAD2 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Arabidopsis APC/C subunits;
CC URL="http://personal.rhul.ac.uk/ujba/110/apc/APC.htm";
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DR EMBL; AB023041; BAB01061.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77097.1; -; Genomic_DNA.
DR EMBL; BT010608; AAQ89630.1; -; mRNA.
DR EMBL; AK175296; BAD43059.1; -; mRNA.
DR EMBL; AK176363; BAD44126.1; -; mRNA.
DR RefSeq; NP_189227.1; NM_113502.3. [Q9LU93-1]
DR AlphaFoldDB; Q9LU93; -.
DR SMR; Q9LU93; -.
DR BioGRID; 7526; 31.
DR IntAct; Q9LU93; 26.
DR STRING; 3702.AT3G25980.1; -.
DR PaxDb; Q9LU93; -.
DR PRIDE; Q9LU93; -.
DR ProteomicsDB; 238511; -. [Q9LU93-1]
DR EnsemblPlants; AT3G25980.1; AT3G25980.1; AT3G25980. [Q9LU93-1]
DR GeneID; 822195; -.
DR Gramene; AT3G25980.1; AT3G25980.1; AT3G25980. [Q9LU93-1]
DR KEGG; ath:AT3G25980; -.
DR Araport; AT3G25980; -.
DR TAIR; locus:2092125; AT3G25980.
DR eggNOG; KOG3285; Eukaryota.
DR HOGENOM; CLU_072097_0_0_1; -.
DR InParanoid; Q9LU93; -.
DR OMA; MLDGNCT; -.
DR OrthoDB; 1197019at2759; -.
DR PhylomeDB; Q9LU93; -.
DR PRO; PR:Q9LU93; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LU93; baseline and differential.
DR Genevisible; Q9LU93; AT.
DR GO; GO:0010369; C:chromocenter; IDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0000776; C:kinetochore; IDA:TAIR.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:UniProtKB.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005876; C:spindle microtubule; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IDA:TAIR.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IMP:TAIR.
DR Gene3D; 3.30.900.10; -; 1.
DR InterPro; IPR003511; HORMA_dom.
DR InterPro; IPR036570; HORMA_dom_sf.
DR InterPro; IPR027097; Mad2.
DR InterPro; IPR045091; Mad2-like.
DR PANTHER; PTHR11842; PTHR11842; 1.
DR PANTHER; PTHR11842:SF11; PTHR11842:SF11; 1.
DR Pfam; PF02301; HORMA; 1.
DR SUPFAM; SSF56019; SSF56019; 1.
DR PROSITE; PS50815; HORMA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW Cytoplasm; Cytoskeleton; Kinetochore; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..209
FT /note="Mitotic spindle checkpoint protein MAD2"
FT /id="PRO_0000126121"
FT DOMAIN 15..198
FT /note="HORMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00109"
SQ SEQUENCE 209 AA; 23731 MW; 91473FD5334C7A99 CRC64;
MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFVKVKKY GLPMLLIEDE
SVKSFMSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER WNFRIETDNE VVDKGVSREK
SDKEIMREIQ AIMRQVASSV TYLPCLDETC VFDVLAYTDT DVAVPFTWIE SDPKLIANPQ
MVKLHGFDTK IHKVDTLVSY KNDEWDEEE
