ID   MAD2_MAIZE              Reviewed;         208 AA.
AC   Q9XFH3;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Mitotic spindle checkpoint protein MAD2;
GN   Name=MAD2;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10225945; DOI=10.1083/jcb.145.3.425;
RA   Yu H.-G., Muszynski M.G., Dawe R.K.;
RT   "The maize homologue of the cell cycle checkpoint protein MAD2 reveals
RT   kinetochore substructure and contrasting mitotic and meiotic localization
RT   patterns.";
RL   J. Cell Biol. 145:425-435(1999).
CC   -!- FUNCTION: Required for the execution of the mitotic checkpoint which
CC       monitors the process of kinetochore-spindle attachment and delays the
CC       onset of anaphase when this process is not complete. It inhibits the
CC       activity of the anaphase promoting complex by sequestering CDC20 until
CC       all chromosomes are aligned at the metaphase plate (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MAD2 family. {ECO:0000305}.
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DR   EMBL; AF143681; AAD30555.1; -; mRNA.
DR   RefSeq; NP_001105618.1; NM_001112148.1.
DR   AlphaFoldDB; Q9XFH3; -.
DR   SMR; Q9XFH3; -.
DR   STRING; 4577.GRMZM2G047143_P01; -.
DR   PaxDb; Q9XFH3; -.
DR   PRIDE; Q9XFH3; -.
DR   ProMEX; Q9XFH3; -.
DR   GeneID; 542621; -.
DR   KEGG; zma:542621; -.
DR   eggNOG; KOG3285; Eukaryota.
DR   HOGENOM; CLU_072097_0_0_1; -.
DR   OMA; MLDGNCT; -.
DR   OrthoDB; 1197019at2759; -.
DR   Proteomes; UP000007305; Chromosome 10.
DR   ExpressionAtlas; Q9XFH3; baseline and differential.
DR   Genevisible; Q9XFH3; ZM.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   Gene3D; 3.30.900.10; -; 1.
DR   InterPro; IPR003511; HORMA_dom.
DR   InterPro; IPR036570; HORMA_dom_sf.
DR   InterPro; IPR027097; Mad2.
DR   InterPro; IPR045091; Mad2-like.
DR   PANTHER; PTHR11842; PTHR11842; 1.
DR   PANTHER; PTHR11842:SF11; PTHR11842:SF11; 1.
DR   Pfam; PF02301; HORMA; 1.
DR   SUPFAM; SSF56019; SSF56019; 1.
DR   PROSITE; PS50815; HORMA; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..208
FT                   /note="Mitotic spindle checkpoint protein MAD2"
FT                   /id="PRO_0000126122"
FT   DOMAIN          14..197
FT                   /note="HORMA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00109"
SQ   SEQUENCE   208 AA;  23505 MW;  E975AF68E47F5037 CRC64;
     MASRSASKDI ITLRGSAAIV SEFFGYAANS ILYNRAVYPE ESFSKVKKYG LTMLLTQDEG
     VKNFIASLTS QLSEWLEAGK LQRIVLVIMS KATSEVLERW NFNIVTDAEV VEKGAIKEKS
     DKEIMREIQA IMRQIASCIT YLPCLDEPCV FDVLAYTDTD VDAPGTWVES DAKLIDNPQM
     VKLHSFDTKI HKVDTLVSYK KDEWDEEE