MAD2_YEAST
ID MAD2_YEAST Reviewed; 196 AA.
AC P40958; D6VWF2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Mitotic spindle checkpoint component MAD2;
DE Short=Mitotic MAD2 protein;
GN Name=MAD2; OrderedLocusNames=YJL030W; ORFNames=J1256;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=8232541; DOI=10.1038/366082a0;
RA Li R., Havel C., Watson J.A., Murray A.W.;
RT "The mitotic feedback control gene MAD2 encodes the alpha-subunit of a
RT prenyltransferase.";
RL Nature 366:82-84(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=1651172; DOI=10.1016/0092-8674(81)90015-5;
RA Li R., Murray A.W.;
RT "Feedback control of mitosis in budding yeast.";
RL Cell 66:519-531(1991).
RN [6]
RP INTERACTION WITH CDC20.
RX PubMed=9461437; DOI=10.1126/science.279.5353.1041;
RA Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S.,
RA Amon A., Murray A.W.;
RT "Budding yeast Cdc20: a target of the spindle checkpoint.";
RL Science 279:1041-1044(1998).
RN [7]
RP INTERACTION WITH MAD1.
RX PubMed=10436016; DOI=10.1091/mbc.10.8.2607;
RA Chen R.H., Brady D.M., Smith D., Murray A.W., Hardwick K.G.;
RT "The spindle checkpoint of budding yeast depends on a tight complex between
RT the Mad1 and Mad2 proteins.";
RL Mol. Biol. Cell 10:2607-2618(1999).
RN [8]
RP INTERACTION WITH BUB3, AND IDENTIFICATION IN THE MCC COMPLEX.
RX PubMed=11726501; DOI=10.1093/emboj/20.23.6648;
RA Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S.;
RT "Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and
RT does not require intact kinetochores.";
RL EMBO J. 20:6648-6659(2001).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20
RP COMPLEX, AND FUNCTION OF THE MCC COMPLEX.
RX PubMed=15879521; DOI=10.1128/ec.4.5.867-878.2005;
RA Poddar A., Stukenberg P.T., Burke D.J.;
RT "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2
RT assemble during mitosis independently of the kinetochore in Saccharomyces
RT cerevisiae.";
RL Eukaryot. Cell 4:867-878(2005).
CC -!- FUNCTION: Central component of the spindle assembly checkpoint which is
CC a feedback control that prevents cells with incompletely assembled
CC spindles from leaving mitosis. Thought to be recruited to unattached
CC kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from
CC the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and
CC presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase
CC activity of the anaphase promoting complex/cyclosome (APC/C) by
CC preventing its activation by CDC20. {ECO:0000269|PubMed:15879521,
CC ECO:0000269|PubMed:8232541}.
CC -!- SUBUNIT: Component of the mitotic checkpoint complex (MCC) which
CC consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20
CC subcomplex, both of which appear to be assembled during mitoisis
CC independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20
CC occurs in a larger amount than MCC. Interacts with CDC20 and BUB3.
CC {ECO:0000269|PubMed:10436016, ECO:0000269|PubMed:11726501,
CC ECO:0000269|PubMed:15879521, ECO:0000269|PubMed:9461437}.
CC -!- INTERACTION:
CC P40958; P26449: BUB3; NbExp=4; IntAct=EBI-10362, EBI-3830;
CC P40958; P26309: CDC20; NbExp=6; IntAct=EBI-10362, EBI-4212;
CC P40958; P40957: MAD1; NbExp=6; IntAct=EBI-10362, EBI-10354;
CC P40958; P47074: MAD3; NbExp=3; IntAct=EBI-10362, EBI-10369;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 1110 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the MAD2 family. {ECO:0000305}.
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DR EMBL; U14132; AAA21385.1; -; Genomic_DNA.
DR EMBL; Z49305; CAA89321.1; -; Genomic_DNA.
DR EMBL; AY558243; AAS56569.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08768.1; -; Genomic_DNA.
DR PIR; S48302; S48302.
DR RefSeq; NP_012504.3; NM_001181464.3.
DR AlphaFoldDB; P40958; -.
DR SMR; P40958; -.
DR BioGRID; 33729; 389.
DR ComplexPortal; CPX-3212; Mitotic checkpoint complex, MAD1-MAD2-BUB1-BUB3 subcomplex.
DR ComplexPortal; CPX-961; Mitotic spindle assembly checkpoint, MAD1-MAD2 complex.
DR ComplexPortal; CPX-962; Mitotic checkpoint complex, CDC20-MAD2 subcomplex.
DR ComplexPortal; CPX-963; Mitotic checkpoint complex, MAD2-MAD3-BUB3-CDC20.
DR DIP; DIP-815N; -.
DR IntAct; P40958; 30.
DR MINT; P40958; -.
DR STRING; 4932.YJL030W; -.
DR MaxQB; P40958; -.
DR PaxDb; P40958; -.
DR PRIDE; P40958; -.
DR EnsemblFungi; YJL030W_mRNA; YJL030W; YJL030W.
DR GeneID; 853422; -.
DR KEGG; sce:YJL030W; -.
DR SGD; S000003567; MAD2.
DR VEuPathDB; FungiDB:YJL030W; -.
DR eggNOG; KOG3285; Eukaryota.
DR GeneTree; ENSGT00940000153395; -.
DR HOGENOM; CLU_072097_0_0_1; -.
DR InParanoid; P40958; -.
DR OMA; MLDGNCT; -.
DR BioCyc; YEAST:G3O-31499-MON; -.
DR Reactome; R-SCE-141405; Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
DR Reactome; R-SCE-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR PRO; PR:P40958; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P40958; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0033597; C:mitotic checkpoint complex; IDA:SGD.
DR GO; GO:1990333; C:mitotic checkpoint complex, CDC20-MAD2 subcomplex; IPI:ComplexPortal.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:SGD.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0044774; P:mitotic DNA integrity checkpoint signaling; IGI:SGD.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IDA:ComplexPortal.
DR GO; GO:0051444; P:negative regulation of ubiquitin-protein transferase activity; IC:ComplexPortal.
DR GO; GO:1902499; P:positive regulation of protein autoubiquitination; IDA:SGD.
DR Gene3D; 3.30.900.10; -; 1.
DR InterPro; IPR003511; HORMA_dom.
DR InterPro; IPR036570; HORMA_dom_sf.
DR InterPro; IPR027097; Mad2.
DR InterPro; IPR045091; Mad2-like.
DR PANTHER; PTHR11842; PTHR11842; 1.
DR PANTHER; PTHR11842:SF11; PTHR11842:SF11; 1.
DR Pfam; PF02301; HORMA; 1.
DR SUPFAM; SSF56019; SSF56019; 1.
DR PROSITE; PS50815; HORMA; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..196
FT /note="Mitotic spindle checkpoint component MAD2"
FT /id="PRO_0000126115"
FT DOMAIN 8..192
FT /note="HORMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00109"
SQ SEQUENCE 196 AA; 22284 MW; EFE59916C5720644 CRC64;
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR
KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ
IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT
FSTNDHKVGA QVSYKY
