ID   MAD50_ORYSJ             Reviewed;         230 AA.
AC   Q9XJ60; Q0DVM3; Q7XB97; Q8H8H3;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=MADS-box transcription factor 50;
DE            Short=OsMADS50;
DE   AltName: Full=Protein AGAMOUS-like 20;
DE   AltName: Full=Protein SUPPRESSOR OF CONSTANS OVEREXPRESSION 1-like;
DE            Short=OsSOC1;
DE   AltName: Full=RMADS208;
GN   Name=MADS50; Synonyms=AGL20, SOC1;
GN   OrderedLocusNames=Os03g0122600, LOC_Os03g03070; ORFNames=OJ1126B12.8;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=cv. Nipponbare; TISSUE=Shoot;
RX   PubMed=10382970; DOI=10.1093/dnares/6.2.123;
RA   Shinozuka Y., Kojima S., Shomura A., Ichimura H., Yano M., Yamamoto K.,
RA   Sasaki T.;
RT   "Isolation and characterization of rice MADS box gene homologues and their
RT   RFLP mapping.";
RL   DNA Res. 6:123-129(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA   Yao Q., Peng R., Xiong A.;
RT   "Isolation and characterization of rice MADS box gene homologs.";
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=17166135; DOI=10.1046/j.1467-7652.2003.00034.x;
RA   Tadege M., Sheldon C.C., Helliwell C.A., Upadhyaya N.M., Dennis E.S.,
RA   Peacock W.J.;
RT   "Reciprocal control of flowering time by OsSOC1 in transgenic Arabidopsis
RT   and by FLC in transgenic rice.";
RL   Plant Biotechnol. J. 1:361-369(2003).
RN   [8]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=15144377; DOI=10.1111/j.1365-313x.2004.02082.x;
RA   Lee S., Kim J., Han J.-J., Han M.-J., An G.;
RT   "Functional analyses of the flowering time gene OsMADS50, the putative
RT   SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog
RT   in rice.";
RL   Plant J. 38:754-764(2004).
CC   -!- FUNCTION: Probable transcription factor active in flowering time
CC       control. May control internode elongation and promote floral transition
CC       phase. May act upstream of the floral regulators MADS1, MADS14, MADS15
CC       and MADS18 in the floral induction pathway.
CC       {ECO:0000269|PubMed:15144377, ECO:0000269|PubMed:17166135}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9XJ60-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9XJ60-2; Sequence=VSP_017787, VSP_017788;
CC   -!- TISSUE SPECIFICITY: Expressed in mature leaves and at low levels in
CC       roots and young panicles. {ECO:0000269|PubMed:15144377,
CC       ECO:0000269|PubMed:17166135}.
CC   -!- DEVELOPMENTAL STAGE: Low expression in the young panicle continues to
CC       decline as the organ mature. {ECO:0000269|PubMed:15144377,
CC       ECO:0000269|PubMed:17166135}.
CC   -!- DISRUPTION PHENOTYPE: Late-flowering phenotype and elongated
CC       internodes. {ECO:0000269|PubMed:15144377}.
CC   -!- MISCELLANEOUS: Early flowering phenotype is observed in overexpressing
CC       plants.
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DR   EMBL; AB003328; BAA81886.1; -; mRNA.
DR   EMBL; AY332476; AAQ01162.1; -; mRNA.
DR   EMBL; AY551914; AAS59820.1; -; mRNA.
DR   EMBL; AC098695; AAN74833.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF10715.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS82045.1; -; Genomic_DNA.
DR   RefSeq; XP_015630979.1; XM_015775493.1. [Q9XJ60-1]
DR   AlphaFoldDB; Q9XJ60; -.
DR   SMR; Q9XJ60; -.
DR   BioGRID; 800482; 2.
DR   STRING; 4530.OS03T0122600-01; -.
DR   PaxDb; Q9XJ60; -.
DR   PRIDE; Q9XJ60; -.
DR   EnsemblPlants; Os03t0122600-01; Os03t0122600-01; Os03g0122600. [Q9XJ60-1]
DR   EnsemblPlants; Os03t0122600-02; Os03t0122600-02; Os03g0122600. [Q9XJ60-1]
DR   GeneID; 4331445; -.
DR   Gramene; Os03t0122600-01; Os03t0122600-01; Os03g0122600. [Q9XJ60-1]
DR   Gramene; Os03t0122600-02; Os03t0122600-02; Os03g0122600. [Q9XJ60-1]
DR   KEGG; osa:4331445; -.
DR   eggNOG; KOG0014; Eukaryota.
DR   HOGENOM; CLU_053053_0_4_1; -.
DR   InParanoid; Q9XJ60; -.
DR   OMA; WVFTTPT; -.
DR   OrthoDB; 1307109at2759; -.
DR   PlantReactome; R-OSA-8934036; Long day regulated expression of florigens.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q9XJ60; OS.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..230
FT                   /note="MADS-box transcription factor 50"
FT                   /id="PRO_0000229916"
FT   DOMAIN          1..61
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          86..176
FT                   /note="K-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT   REGION          209..230
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..79
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_017787"
FT   VAR_SEQ         80..87
FT                   /note="KTVQQDIE -> MCIVFEIM (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_017788"
SQ   SEQUENCE   230 AA;  26181 MW;  4DEE81FB6B2EDD42 CRC64;
     MVRGKTQMKR IENPTSRQVT FSKRRNGLLK KAFELSVLCD AEVALIVFSP RGKLYEFASA
     STQKTIERYR TYTKENIGNK TVQQDIEQVK ADADGLAKKL EALETYKRKL LGEKLDECSI
     EELHSLEVKL ERSLISIRGR KTKLLEEQVA KLREKEMKLR KDNEELREKC KNQPPLSAPL
     TVRAEDENPD RNINTTNDNM DVETELFIGL PGRSRSSGGA AEDSQAMPHS