ID   MADB_MALRU              Reviewed;         401 AA.
AC   O06923;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Carboxybiotin decarboxylase;
DE            EC=7.2.4.1 {ECO:0000269|PubMed:11902724};
DE   AltName: Full=Carboxybiotin protein decarboxylase;
GN   Name=madB;
OS   Malonomonas rubra.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Desulfuromonadaceae; Malonomonas.
OX   NCBI_TaxID=57040;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RX   PubMed=9128730; DOI=10.1111/j.1432-1033.1997.00103.x;
RA   Berg M., Hilbi H., Dimroth P.;
RT   "Sequence of a gene cluster from Malonomonas rubra encoding components of
RT   the malonate decarboxylase Na+ pump and evidence for their function.";
RL   Eur. J. Biochem. 245:103-115(1997).
RN   [2]
RP   CATALYTIC ACTIVITY.
RX   PubMed=11902724; DOI=10.1046/j.1365-2958.1997.4611824.x;
RA   Dimroth P., Hilbi H.;
RT   "Enzymic and genetic basis for bacterial growth on malonate.";
RL   Mol. Microbiol. 25:3-10(1997).
CC   -!- FUNCTION: Beta subunit of the biotin-dependent malonate decarboxylase
CC       multienzyme complex (EC 7.2.4.4). Acts as an integral membrane-bound
CC       carboxybiotin protein decarboxylase by releasing the carboxyl group of
CC       the carboxylated biotin carrier MADF. The free energy of the
CC       decarboxylation reaction is used to pump Na(+) out of the cell.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + n Na(+)(in) =
CC         CO2 + N(6)-biotinyl-L-lysyl-[protein] + n Na(+)(out);
CC         Xref=Rhea:RHEA:43336, Rhea:RHEA-COMP:10505, Rhea:RHEA-COMP:10506,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:29101,
CC         ChEBI:CHEBI:83144, ChEBI:CHEBI:83145; EC=7.2.4.1;
CC         Evidence={ECO:0000269|PubMed:11902724};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; U87980; AAC45399.1; -; Genomic_DNA.
DR   AlphaFoldDB; O06923; -.
DR   SMR; O06923; -.
DR   TCDB; 3.B.1.1.4; the na(+)-transporting carboxylic acid decarboxylase (nat-dc) family.
DR   KEGG; ag:AAC45399; -.
DR   BioCyc; MetaCyc:MON-14257; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015451; F:decarboxylation-driven active transmembrane transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0016829; F:lyase activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR   InterPro; IPR017558; Malonate_biotin.
DR   InterPro; IPR005661; OadB_MmdB.
DR   PANTHER; PTHR35806; PTHR35806; 1.
DR   Pfam; PF03977; OAD_beta; 1.
DR   PIRSF; PIRSF015658; MmdB_OadB; 1.
DR   TIGRFAMs; TIGR03136; malonate_biotin; 1.
DR   TIGRFAMs; TIGR01109; Na_pump_decarbB; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Translocase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..401
FT                   /note="Carboxybiotin decarboxylase"
FT                   /id="PRO_0000424275"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   401 AA;  43024 MW;  5E69B195E29748CD CRC64;
     MEQLMSLFPA ISTLFTQDPV ISITRIALII FGFFLSYFGF KRTLEPLIMV PMGLGMIAIN
     AGVLFLEAGV VGTIHLDPLV SEPSVLVNLM QVNWLQPVYN FTFSNGLIAC IVFFGIGAMS
     DISFILIRPW ASIIVALFAE MGTFATLIIG IKMGLLPNEA AAVATIGGAD GPMVLFASLI
     LAKDLFVPIA IIAYLYLSLT YAGYPYLIKL LVPKKYRGLE VEMDFPEVSQ RSKFVFSVLA
     CMLLCLLLPV ASPLILSFFL GIAIKEAQIE PFQNLLETTL TYGSTLFLGL LLGALCEAKT
     ILDPKISLIV VLGITALLIS GIGGVLGGWI VYWFSKGKFN PVIGIAGVSC LPTTAKIAQK
     TVTEENPYAV ILPLAMGAGV CGLIVSAIAT GVFISTLFLL N