ID   MADS3_ORYSJ             Reviewed;         236 AA.
AC   Q40704; Q6Q7W1;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=MADS-box transcription factor 3;
DE   AltName: Full=OsMADS3;
DE   AltName: Full=Protein AGAMOUS-like;
DE   AltName: Full=RMADS222;
GN   Name=MADS3; Synonyms=RAG; OrderedLocusNames=Os01g0201700, LOC_Os01g10504;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Flower;
RX   PubMed=7579155; DOI=10.1007/bf00019114;
RA   Kang H.-G., Noh Y.-S., Chung Y.-Y., Costa M.A., An K., An G.;
RT   "Phenotypic alterations of petal and sepal by ectopic expression of a rice
RT   MADS box gene in tobacco.";
RL   Plant Mol. Biol. 29:1-10(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Yao Q., Peng R., Xiong A.;
RT   "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT   factor family in rice.";
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   FUNCTION.
RX   PubMed=9869408; DOI=10.1023/a:1006051911291;
RA   Kang H.-G., Jeon J.-S., Lee S., An G.;
RT   "Identification of class B and class C floral organ identity genes from
RT   rice plants.";
RL   Plant Mol. Biol. 38:1021-1029(1998).
RN   [8]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=10945340; DOI=10.1093/pcp/41.6.710;
RA   Kyozuka J., Kobayashi T., Morita M., Shimamoto K.;
RT   "Spatially and temporally regulated expression of rice MADS box genes with
RT   similarity to Arabidopsis class A, B and C genes.";
RL   Plant Cell Physiol. 41:710-718(2000).
RN   [9]
RP   FUNCTION.
RX   PubMed=11828031; DOI=10.1093/pcp/pcf010;
RA   Kyozuka J., Shimamoto K.;
RT   "Ectopic expression of OsMADS3, a rice ortholog of AGAMOUS, caused a
RT   homeotic transformation of lodicules to stamens in transgenic rice
RT   plants.";
RL   Plant Cell Physiol. 43:130-135(2002).
RN   [10]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX   PubMed=16326928; DOI=10.1105/tpc.105.037200;
RA   Yamaguchi T., Lee D.-Y., Miyao A., Hirochika H., An G., Hirano H.;
RT   "Functional diversification of the two C-class MADS box genes OSMADS3 and
RT   OSMADS58 in Oryza sativa.";
RL   Plant Cell 18:15-28(2006).
CC   -!- FUNCTION: Probable transcription factor involved in the development of
CC       floral organs. Acts as C-class protein in association with MADS58.
CC       Involved in the control of lodicule number (whorl 2), stamen
CC       specification (whorl 3) and floral meristem determinacy (whorl 4), but
CC       not in the regulation of carpel morphogenesis. Plays a more predominant
CC       role in controlling lodicule development and in specifying stamen
CC       identity than MADS58. {ECO:0000269|PubMed:11828031,
CC       ECO:0000269|PubMed:16326928, ECO:0000269|PubMed:9869408}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q40704-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q40704-2; Sequence=VSP_017779;
CC       Name=3;
CC         IsoId=Q40704-3; Sequence=VSP_017777, VSP_017778;
CC   -!- TISSUE SPECIFICITY: Expressed in lemmas, paleas and lodicules.
CC       {ECO:0000269|PubMed:10945340}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at early stage of flower development in
CC       stamen anlagen before stamen initiation, and at later stage in lodicule
CC       and ovule primordia initiation region. {ECO:0000269|PubMed:10945340,
CC       ECO:0000269|PubMed:16326928}.
CC   -!- DISRUPTION PHENOTYPE: Homeotic transformation of stamens into
CC       lodicules, and ectopic development of lodicules where they do not form
CC       in the wild-type. {ECO:0000269|PubMed:16326928}.
CC   -!- MISCELLANEOUS: The transformation of lodicules into stamens is observed
CC       in overexpressing plants.
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DR   EMBL; L37528; AAA99964.1; -; mRNA.
DR   EMBL; AY553724; AAS60204.1; -; mRNA.
DR   EMBL; AP003105; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK108568; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; S59480; S59480.
DR   AlphaFoldDB; Q40704; -.
DR   SMR; Q40704; -.
DR   IntAct; Q40704; 3.
DR   STRING; 4530.OS01T0201700-01; -.
DR   PaxDb; Q40704; -.
DR   PRIDE; Q40704; -.
DR   eggNOG; KOG0014; Eukaryota.
DR   InParanoid; Q40704; -.
DR   PlantReactome; R-OSA-9609102; Flower development.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0010097; P:specification of stamen identity; TAS:AgBase.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..236
FT                   /note="MADS-box transcription factor 3"
FT                   /id="PRO_0000229893"
FT   DOMAIN          1..61
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          87..178
FT                   /note="K-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT   VAR_SEQ         110..114
FT                   /note="RTIVG -> LTRLA (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_017777"
FT   VAR_SEQ         115..236
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:12869764"
FT                   /id="VSP_017778"
FT   VAR_SEQ         231..236
FT                   /note="QQPAFN -> SRPSISFGVDTVRTHVR (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_017779"
SQ   SEQUENCE   236 AA;  27141 MW;  241BD9BCE2DAD35F CRC64;
     MGRGKIEIKR IENTTNRQVT FCKRRNGLLK KAYELSVLCD AEVALIVFSS RGRLYEYANN
     SVKSTVERYK KANSDTSNSG TVAEVNAQHY QQESSKLRQQ ISSLQNANSR TIVGDSINTM
     SLRDLKQVEN RLEKGIAKIR ARKNELLYAE VEYMQKREVE LQNDNMYLRS KVVENERGQQ
     PLNMMGAAST SEYDHMVNNP YDSRNFLQVN IMQQPQHYAH QLQPTTLQLG QQPAFN