MADS6_ORYSJ
ID MADS6_ORYSJ Reviewed; 250 AA.
AC Q6EU39; A0A0P0VN09; O24228; Q0DYM5;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=MADS-box transcription factor 6;
DE AltName: Full=OsMADS6;
DE AltName: Full=Protein MOSAIC FLORAL ORGANS 1;
GN Name=MADS6; Synonyms=MFO1; OrderedLocusNames=Os02g0682200, LOC_Os02g45770;
GN ORFNames=OJ1123_G04.6, OsJ_07942;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MADS1; MADS5; MADS7; MADS8;
RP MADS14; MADS15; MADS17 AND MADS18, AND TISSUE SPECIFICITY.
RX PubMed=10444103; DOI=10.1104/pp.120.4.1193;
RA Moon Y.-H., Kang H.-G., Jung J.-Y., Jeon J.-S., Sung S.-K., An G.;
RT "Determination of the motif responsible for interaction between the rice
RT APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system.";
RL Plant Physiol. 120:1193-1204(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF ARG-24, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19820190; DOI=10.1105/tpc.109.068742;
RA Ohmori S., Kimizu M., Sugita M., Miyao A., Hirochika H., Uchida E.,
RA Nagato Y., Yoshida H.;
RT "MOSAIC FLORAL ORGANS1, an AGL6-like MADS box gene, regulates floral organ
RT identity and meristem fate in rice.";
RL Plant Cell 21:3008-3025(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION.
RX AGRICOLA=IND22436162; DOI=10.1023/A:1011388620872;
RA Jeon J.-S., Lee S., Nam J., Jung K.-H., Yang W.-S., Yi G.-H., Oh B.-G.,
RA An G.;
RT "Production of transgenic rice plants showing reduced heading date and
RT plant height by ectopic expression of rice MADS-box genes.";
RL Mol. Breed. 6:581-592(2000).
RN [9]
RP INTERACTION WITH MADS18 AND NF-YB1.
RX PubMed=11971906; DOI=10.1074/jbc.m202546200;
RA Masiero S., Imbriano C., Ravasio F., Favaro R., Pelucchi N., Gorla M.S.,
RA Mantovani R., Colombo L., Kater M.M.;
RT "Ternary complex formation between MADS-box transcription factors and the
RT histone fold protein NF-YB.";
RL J. Biol. Chem. 277:26429-26435(2002).
RN [10]
RP INTERACTION WITH MADS13, AND DEVELOPMENTAL STAGE.
RX PubMed=12395189; DOI=10.1007/s00438-002-0746-6;
RA Favaro R., Immink R.G., Ferioli V., Bernasconi B., Byzova M.,
RA Angenent G.C., Kater M.M., Colombo L.;
RT "Ovule-specific MADS-box proteins have conserved protein-protein
RT interactions in monocot and dicot plants.";
RL Mol. Genet. Genomics 268:152-159(2002).
RN [11]
RP INTERACTION WITH MADS16.
RX PubMed=12905025; DOI=10.1007/s00425-003-1066-8;
RA Lee S., Jeon J.-S., An K., Moon Y.-H., Lee S.-H., Chung Y.-Y., An G.;
RT "Alteration of floral organ identity in rice through ectopic expression of
RT OsMADS16.";
RL Planta 217:904-911(2003).
RN [12]
RP INTERACTION WITH MADS18.
RX PubMed=15299121; DOI=10.1104/pp.104.045039;
RA Fornara F., Parenicova L., Falasca G., Pelucchi N., Masiero S.,
RA Ciannamea S., Lopez-Dee Z.P., Altamura M.M., Colombo L., Kater M.M.;
RT "Functional characterization of OsMADS18, a member of the AP1/SQUA
RT subfamily of MADS box genes.";
RL Plant Physiol. 135:2207-2219(2004).
CC -!- FUNCTION: Probable transcription factor. Regulates floral organ
CC identity and floral meristem determinacy. May be involved in the
CC control of flowering time. {ECO:0000269|PubMed:19820190,
CC ECO:0000269|Ref.8}.
CC -!- SUBUNIT: May interact with the K-box of MADS1, MADS5, MADS7, MADS8,
CC MADS14, MADS15, MADS16, MADS17 and MADS18. May interact with MADS13.
CC The dimer composed of MADS6 and MADS18 forms a ternary complex with
CC histone fold protein NF-YB1.
CC -!- INTERACTION:
CC Q6EU39; Q10PZ9: MADS1; NbExp=4; IntAct=EBI-627980, EBI-627957;
CC Q6EU39; Q10CQ1: MADS14; NbExp=5; IntAct=EBI-627980, EBI-627890;
CC Q6EU39; Q6Q9I2: MADS15; NbExp=3; IntAct=EBI-627980, EBI-627918;
CC Q6EU39; Q0D4T4: MADS18; NbExp=3; IntAct=EBI-627980, EBI-627967;
CC Q6EU39; Q0DEB8: MADS5; NbExp=3; IntAct=EBI-627980, EBI-627927;
CC Q6EU39; Q0J466: MADS7; NbExp=5; IntAct=EBI-627980, EBI-627872;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the floral meristem. Highly expressed
CC in lodicules. Expressed in palea and pistil. Weakly expressed in
CC carpels, empty glumes and stamens. Not detected in lemmas.
CC {ECO:0000269|PubMed:10444103, ECO:0000269|PubMed:19820190}.
CC -!- DEVELOPMENTAL STAGE: Expressed during ovule development in the inner
CC and outer integuments. Not detected in young panicles.
CC {ECO:0000269|PubMed:12395189, ECO:0000269|PubMed:19820190}.
CC -!- DISRUPTION PHENOTYPE: Abnormal and almost sterile florets. Mosaic
CC organs and disturbed identities of palea and lodicule. Extra carpels or
CC spikelets. {ECO:0000269|PubMed:19820190}.
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DR EMBL; U78782; AAB64250.1; -; mRNA.
DR EMBL; FJ666318; ACV89861.1; -; mRNA.
DR EMBL; AP004178; BAD27830.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09663.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80308.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57586.1; -; Genomic_DNA.
DR EMBL; AK069103; BAG91254.1; -; mRNA.
DR PIR; T04167; T04167.
DR RefSeq; XP_015623947.1; XM_015768461.1.
DR AlphaFoldDB; Q6EU39; -.
DR SMR; Q6EU39; -.
DR IntAct; Q6EU39; 15.
DR STRING; 4530.OS02T0682200-01; -.
DR PaxDb; Q6EU39; -.
DR PRIDE; Q6EU39; -.
DR EnsemblPlants; Os02t0682200-01; Os02t0682200-01; Os02g0682200.
DR GeneID; 4330328; -.
DR Gramene; Os02t0682200-01; Os02t0682200-01; Os02g0682200.
DR KEGG; osa:4330328; -.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_2_1; -.
DR InParanoid; Q6EU39; -.
DR OMA; HANPIDC; -.
DR OrthoDB; 1353656at2759; -.
DR PlantReactome; R-OSA-9609102; Flower development.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6EU39; OS.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; TAS:AgBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; TAS:AgBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IMP:AgBase.
DR GO; GO:0010582; P:floral meristem determinacy; IMP:AgBase.
DR GO; GO:0048437; P:floral organ development; IDA:AgBase.
DR GO; GO:0048449; P:floral organ formation; IGI:AgBase.
DR GO; GO:0048457; P:floral whorl morphogenesis; IMP:AgBase.
DR GO; GO:0048459; P:floral whorl structural organization; IMP:AgBase.
DR GO; GO:0080060; P:integument development; IMP:AgBase.
DR GO; GO:0010022; P:meristem determinacy; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0048509; P:regulation of meristem development; IGI:AgBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048316; P:seed development; IMP:AgBase.
DR GO; GO:0080112; P:seed growth; IMP:AgBase.
DR GO; GO:0010094; P:specification of carpel identity; IMP:AgBase.
DR GO; GO:0010093; P:specification of floral organ identity; IMP:UniProtKB.
DR GO; GO:0048833; P:specification of floral organ number; IMP:AgBase.
DR GO; GO:0048455; P:stamen formation; IMP:AgBase.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..250
FT /note="MADS-box transcription factor 6"
FT /id="PRO_0000229895"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 86..176
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT MUTAGEN 24
FT /note="R->H: In mfo1-1; loss of function."
FT /evidence="ECO:0000269|PubMed:19820190"
SQ SEQUENCE 250 AA; 28437 MW; 7E574BD86A48220C CRC64;
MGRGRVELKR IENKINRQVT FSKRRNGLLK KAYELSVLCD AEVALIIFSS RGKLYEFGSA
GITKTLERYQ HCCYNAQDSN NALSETQSWY HEMSKLKAKF EALQRTQRHL LGEDLGPLSV
KELQQLEKQL ECALSQARQR KTQLMMEQVE ELRRKERQLG EINRQLKHKL EVEGSTSNYR
AMQQASWAQG AVVENGAAYV QPPPHSAAMD SEPTLQIGYP HQFVPAEANT IQRSTAPAGA
ENNFMLGWVL
