ID   MAEL1_DROPS             Reviewed;         371 AA.
AC   B5DR03;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Protein maelstrom 1;
GN   Name=mael1; ORFNames=GA28467;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; CH379070; EDY73922.1; -; Genomic_DNA.
DR   RefSeq; XP_002135295.1; XM_002135259.2.
DR   RefSeq; XP_015043537.1; XM_015188051.1.
DR   AlphaFoldDB; B5DR03; -.
DR   SMR; B5DR03; -.
DR   STRING; 7237.FBpp0286632; -.
DR   EnsemblMetazoa; FBtr0288194; FBpp0286632; FBgn0249827.
DR   EnsemblMetazoa; FBtr0374140; FBpp0335782; FBgn0249827.
DR   GeneID; 6899927; -.
DR   KEGG; dpo:Dpse_GA28467; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   HOGENOM; CLU_044134_0_0_1; -.
DR   InParanoid; B5DR03; -.
DR   OMA; TLCDEFC; -.
DR   Proteomes; UP000001819; Chromosome X.
DR   Bgee; FBgn0249827; Expressed in female reproductive system and 2 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Reference proteome; Repressor;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation.
FT   CHAIN           1..371
FT                   /note="Protein maelstrom 1"
FT                   /id="PRO_0000367303"
FT   DNA_BIND        2..68
FT                   /note="HMG box"
SQ   SEQUENCE   371 AA;  41931 MW;  66C4C4C63005ADC9 CRC64;
     MAQNKPNAFM AFVADWRACN RFGRGLSTSE AVAKCGPIWE GMSDRERGPY KSKAKDSNVL
     ERESKTERLN CPGVAFSKMQ VEKNEAIDAE LQMKRNIKRI VLKATNSMKL EEEEFLFVSF
     NYFTKALNGD VYQPAELSAC RFSLKGGISS NYSTMINPGH IIFGQTSDAQ DHSRTTHKLP
     LPPNAMGEKN LGNLYSDTLK WLSASNDEED EQYDHPVIVY TTPELMPVVK SCFRYLACEG
     DTDKHAKKII VYDICYLFLT LKKTVLDLVG VPSDHMNIHV TNSFFRRDFF EFSSGIACDY
     HEEVDRTKYC TKSMVLRWGY MISHYICGDL AIPLQPRKHV PIEVKHSYTV TPGDSSLALD
     GTSTDSGYSG Y