ID   MAEL2_DROAN             Reviewed;         468 AA.
AC   B3MZY6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Protein maelstrom 2;
GN   Name=mael2; ORFNames=GF20523;
OS   Drosophila ananassae (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14024-0371.13;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; CH902636; EDV44771.1; -; Genomic_DNA.
DR   RefSeq; XP_001953458.2; XM_001953422.2.
DR   AlphaFoldDB; B3MZY6; -.
DR   SMR; B3MZY6; -.
DR   STRING; 7217.FBpp0123715; -.
DR   GeneID; 6503226; -.
DR   KEGG; dan:6503226; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   HOGENOM; CLU_044134_0_0_1; -.
DR   InParanoid; B3MZY6; -.
DR   OMA; YANIVEY; -.
DR   PhylomeDB; B3MZY6; -.
DR   Proteomes; UP000007801; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Reference proteome; Repressor;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation.
FT   CHAIN           1..468
FT                   /note="Protein maelstrom 2"
FT                   /id="PRO_0000367296"
FT   DNA_BIND        2..69
FT                   /note="HMG box"
FT   REGION          374..393
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        379..393
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   468 AA;  53409 MW;  9CFDC53357E08222 CRC64;
     MPPKKHSGFM MFVNEWRDNN PEGRNLSIAQ AVSRCGSIWE KMTAQQRGPY NSGAKNADVL
     TRVKKERLNC HGQVLSQVEL EEREMAESQI HMKRCTERIV MDAKRSHDLE NTKFVFVAFN
     YFTKAFTTDV YVPAEFSASE YSFNEGIMSV YSTLIDPGQI IFGQGSDAQH HSSTTHNLPL
     PPNALGEKNM GKLYRNILEY LSKIQEGKDA TKPFVVFTKT DMVPFVKSCF RYLACENQDG
     SYENGDQIQV LDIQYLLFIQ KKEVLDIAGV SDEKINLYVT DAYFLKDFFE FTPEISCQYH
     EENDRSKYCT QSLVMRWAYT FSDYMCSDLA ISVQPGKHIP PKTKPNYRVI PANSSVHESS
     FDSFYSIPAS CEKKEDSPTV LSPASSRRSL ASSPYVPTDH TSFVGDLNKK DEFPSLDQRN
     KLAALIDLIQ FFYRCPVQLC RPLFSAGYHL NDALEPPHDP RDLYGRPL