ID   MAEL2_DROPE             Reviewed;         438 AA.
AC   B4GN43;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Protein maelstrom 2;
GN   Name=mael2; ORFNames=GL13552;
OS   Drosophila persimilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSH-3 / Tucson 14011-0111.49;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDW39169.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CH479186; EDW39169.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_002020357.1; XM_002020321.1.
DR   AlphaFoldDB; B4GN43; -.
DR   SMR; B4GN43; -.
DR   STRING; 7234.FBpp0177659; -.
DR   EnsemblMetazoa; FBtr0179167; FBpp0177659; FBgn0151157.
DR   GeneID; 6595291; -.
DR   KEGG; dpe:6595291; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   Proteomes; UP000008744; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Reference proteome; Repressor;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation.
FT   CHAIN           1..438
FT                   /note="Protein maelstrom 2"
FT                   /id="PRO_0000367302"
FT   DNA_BIND        2..69
FT                   /note="HMG box"
FT   REGION          374..438
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        378..393
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   438 AA;  49768 MW;  924F2A2516B4F9C2 CRC64;
     MAPKKRNGFM TFVKEWQANN PVARGLSNSE AVAKCDPIWK SMGDQERGPY NSMAKNANVL
     ERTAKKERLN CLGGSVAEME IEKNEAISAE LQMKRKIERI ILTAKNSMEL ENEEFVFVSF
     NYFTKALTGD IYVPAEFSAC RYSLKGGISS NYSTMINPGH IIYGQSRDAQ DHSKTTHKLP
     LPPQAFGETN MGKLYIDIFN WLSVRNEEKL DQDPVIVYTT PELMPVVKSC FRYLASEAEI
     DEDERKIMVF DIHHLFYTLK KSVLDVAGVT NDRINFHVTN NFFVKDFFEY TEGISCDYHE
     KIDRSKYCTN SMVKRWGFTF SDYMCADLAI PLQPGKHIPL KVKPNYTITP ASSSTNFDEI
     SLDSYYSAPP RIQKEMGSRD LSPSSSHQSV SRAYVPRDHS VYGGTLDSDE EFPSLGGRRR
     QLPDKSHFNM GAGKKIAR