ID   MAEL_BOVIN              Reviewed;         420 AA.
AC   Q32KV2;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Protein maelstrom homolog;
GN   Name=MAEL;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role during spermatogenesis by repressing
CC       transposable elements and preventing their mobilization, which is
CC       essential for the germline integrity. Acts via the piRNA metabolic
CC       process, which mediates the repression of transposable elements during
CC       meiosis by forming complexes composed of piRNAs and Piwi proteins and
CC       governs the methylation and subsequent repression of transposons. Its
CC       association with piP-bodies suggests a participation in the secondary
CC       piRNAs metabolic process. Required for the localization of germ-cell
CC       factors to the meiotic nuage (By similarity).
CC       {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SUBUNIT: Interacts with SMARCB1, SIN3B and DDX4. Interacts with piRNA-
CC       associated proteins TDRD1, PIWIL1 and PIWIL2 (By similarity). Interacts
CC       with TEX19 (By similarity). {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BVN9}. Nucleus
CC       {ECO:0000250|UniProtKB:Q8BVN9}. Note=Component of the meiotic nuage,
CC       also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. Specifically localizes to
CC       piP-bodies, a subset of the nuage which contains secondary piRNAs (By
CC       similarity). {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; BC109912; AAI09913.1; -; mRNA.
DR   RefSeq; NP_001033282.1; NM_001038193.2.
DR   AlphaFoldDB; Q32KV2; -.
DR   SMR; Q32KV2; -.
DR   STRING; 9913.ENSBTAP00000034004; -.
DR   PaxDb; Q32KV2; -.
DR   PRIDE; Q32KV2; -.
DR   GeneID; 541073; -.
DR   KEGG; bta:541073; -.
DR   CTD; 84944; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   InParanoid; Q32KV2; -.
DR   OrthoDB; 859881at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0071547; C:piP-body; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0043046; P:DNA methylation involved in gamete generation; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Reference proteome; RNA-mediated gene silencing; Spermatogenesis.
FT   CHAIN           1..420
FT                   /note="Protein maelstrom homolog"
FT                   /id="PRO_0000232500"
FT   DNA_BIND        4..73
FT                   /note="HMG box"
FT   REGION          62..94
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          341..372
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          392..420
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   420 AA;  47327 MW;  69BB8E374C3D3688 CRC64;
     MPNRRASRNA YYFFVQEKIP ELRRRGLPVA RVADAIPYCS ADWALLKEEE KEKYAEMARE
     WRAAQGKDSG PSEKQKPVCT PLRKPGMLVP KQNVSPPDLS GLSLKSDQAL LGGIFYFLNI
     FSHGELPPHC EQRFLPCEIG CVKYSLQEGI MADFHSFINP GDSSHKIPIS HFESGHDQAT
     VIENLYRFIH PNPGNWPPIY CKSDDRARVN WCLKHMAKSS EIRQDLELLT VEDLVVGIYQ
     QKFLKEPSKT WVRSLLEVAM WDYSSNTRCK WHEENDILFC ALAVCKKIAN CISNSLATLF
     GIQLTEAHVP LQDYEASNSV TPKMVVLDAG RYQKLRVESP GFSHFSSSNQ EQRSNTPTGD
     YPSGVKISGQ NSSVRGRGIT RLLESISSSS SNIHKFSNCE TPVSPYTSPK HGYKSFSSLS