ID   MAEL_DROER              Reviewed;         465 AA.
AC   B3NEF0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Protein maelstrom;
GN   Name=mael; ORFNames=GG16274;
OS   Drosophila erecta (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7220;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14021-0224.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; CH954178; EDV52785.1; -; Genomic_DNA.
DR   RefSeq; XP_001973759.1; XM_001973723.2.
DR   AlphaFoldDB; B3NEF0; -.
DR   SMR; B3NEF0; -.
DR   STRING; 7220.FBpp0134820; -.
DR   PRIDE; B3NEF0; -.
DR   EnsemblMetazoa; FBtr0136328; FBpp0134820; FBgn0108509.
DR   GeneID; 6544104; -.
DR   KEGG; der:6544104; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   HOGENOM; CLU_044134_0_0_1; -.
DR   OMA; YANIVEY; -.
DR   OrthoDB; 859881at2759; -.
DR   PhylomeDB; B3NEF0; -.
DR   Proteomes; UP000008711; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Repressor; RNA-mediated gene silencing; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..465
FT                   /note="Protein maelstrom"
FT                   /id="PRO_0000367297"
FT   DNA_BIND        2..69
FT                   /note="HMG box"
FT   REGION          373..394
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          419..443
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        378..394
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   465 AA;  52343 MW;  B15541C67F03B3E2 CRC64;
     MAPKKHSAFM VFVTEWRNHN VEGRRMTLPQ AVSHCGTIWE KMTAQQRGPY QSGAKDADVA
     DRAKRERLNC HGQGIAQVDQ VQKEAAESLM HMKRSTERLV INAKKSHDLE NAKFVFATFN
     YFTKALTTDV YVPAEFAACE YSLKEGIRSI YSSMIDPGQI IFGQGSDALH HSSTTHDLPL
     PPNALGEKNM AKLYRNIIDY LTKCQGGDKP LIVFTPAENI AMVKSCFRYL ECEDDSKDGG
     RTIQVFDIEY LLFILKKEVM DVADLNDEKI NKFVTDAFFK KDFFEFTAGI ACQYHEDNDR
     TKYCTQSMVT RWAYTFSDFM CGDLAITVQP GKHIPAHTKP NYRIISSDAS SLAHESSFDS
     FYSLPGSRVK KENQSEDVLL SSSRPSVESS SYTPTDHTAF TADLSKLCEF PSLGMRNSSK
     HRGLDVSAQR ERNAGAWNLP THSRSIQKFS DNDFSVTGAD GKHKN