ID   MAEL_DROMO              Reviewed;         454 AA.
AC   B4KVI7;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Protein maelstrom;
GN   Name=mael; ORFNames=GI11511;
OS   Drosophila mojavensis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15081-1352.22;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; CH933809; EDW19458.1; -; Genomic_DNA.
DR   RefSeq; XP_002008982.1; XM_002008946.2.
DR   AlphaFoldDB; B4KVI7; -.
DR   SMR; B4KVI7; -.
DR   STRING; 7230.FBpp0160728; -.
DR   EnsemblMetazoa; FBtr0162236; FBpp0160728; FBgn0134271.
DR   GeneID; 6583312; -.
DR   KEGG; dmo:Dmoj_GI11511; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   HOGENOM; CLU_044134_0_0_1; -.
DR   InParanoid; B4KVI7; -.
DR   OMA; YANIVEY; -.
DR   OrthoDB; 859881at2759; -.
DR   PhylomeDB; B4KVI7; -.
DR   Proteomes; UP000009192; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Reference proteome; Repressor;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation.
FT   CHAIN           1..454
FT                   /note="Protein maelstrom"
FT                   /id="PRO_0000367300"
FT   DNA_BIND        2..69
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          336..374
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          405..433
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        345..374
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   454 AA;  51350 MW;  F2483C082EDE61F9 CRC64;
     MAPKKFNGFM MFVNEWREKT AEGRAASLPQ AISKCGEKWK NMNTQQRGPY NKMAKNAEVA
     ARCQVERLNC HGQSLSALER EEMEAAEREL NMKRTTERIV LAGKKRHDLE STKFIFAAFN
     YFTKSMTNDI YIPAEFSACQ FSLKSGICSM YSSHIDPGQL IFGQGSETMH HTKHTHQLPL
     PPNAMGESDI GRLYANIVEY LRACNPDAKP NDPLVVFATP EFMPIVKGCF RYLESDSEEP
     LATIHIYDIQ YLLYVLKLEV LDSVDIRNVT VNRTATDSLF INDYFCYHLG ISCQYHEDID
     RCQYCTQSII SRWCYVFSDF MCGDLAITPL PGKHMPPKQE PKFRVSNPGN ESVNSSRISS
     NFSKTTNKST GKVSNETFTS GQSIVKHEIF DHSAFSADLN AAKDFPSLGG RKPKPNRNPM
     GKDLAPGDPR RAWNVPASKC RVKDFEDSFK MPPS