ID   MAEL_DROVI              Reviewed;         444 AA.
AC   B4LF72;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Protein maelstrom;
GN   Name=mael; ORFNames=GJ13702;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15010-1051.87;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved both in the piRNA and miRNA metabolic processes. As
CC       a component of the meiotic nuage, plays a central role during oogenesis
CC       by repressing transposable elements and preventing their mobilization,
CC       which is essential for the germline integrity. Repression of
CC       transposable elements is mediated via the piRNA metabolic process,
CC       which mediates the repression of transposable elements during meiosis
CC       by forming complexes composed of piRNAs and Piwi proteins and governs
CC       the repression of transposons. As a nuclear component, it is required
CC       for proper differentiation in the germline stem cell (GSC) lineage by
CC       repressing microRNA-7 (miR-7), thereby acting as an indirect regulator
CC       of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene
CC       and repressing its expression; miR-7 repression alleviates the Bam
CC       repression by miR-7, thereby allowing differentiation in the germline
CC       stem cell (GSC) lineage (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Component of the meiotic
CC       nuage, also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; CH940647; EDW70260.1; -; Genomic_DNA.
DR   RefSeq; XP_002047918.1; XM_002047882.2.
DR   AlphaFoldDB; B4LF72; -.
DR   SMR; B4LF72; -.
DR   STRING; 7244.FBpp0228119; -.
DR   EnsemblMetazoa; FBtr0229627; FBpp0228119; FBgn0200923.
DR   GeneID; 6622325; -.
DR   KEGG; dvi:6622325; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   HOGENOM; CLU_044134_0_0_1; -.
DR   InParanoid; B4LF72; -.
DR   OMA; YANIVEY; -.
DR   OrthoDB; 859881at2759; -.
DR   PhylomeDB; B4LF72; -.
DR   Proteomes; UP000008792; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0030718; P:germ-line stem cell population maintenance; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Oogenesis; Reference proteome; Repressor;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation.
FT   CHAIN           1..444
FT                   /note="Protein maelstrom"
FT                   /id="PRO_0000367308"
FT   DNA_BIND        2..69
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
SQ   SEQUENCE   444 AA;  50164 MW;  4568D977D788FDC9 CRC64;
     MPPKKHTGFM MFVQEWRRND AEGRKMSMPQ AVSKCGELWK KMDAQQRGPY NGAAKEADVV
     MRCKVERMNC HGQALSAVQR EEREAADRQM DMKRKIERMV LNGKAQHDLE NTKFIFAAFN
     YFTKAITNDI YIPAEFAACE FSLKSGKSSL YSSHINPGQL IFGQGSDTLH HTSNTHQLPL
     PPNALGEANI GKLYVSIVEY LRGCQDGAGQ PNEPLVVFTS TELVPVVRGC FRYLESDSDE
     LQENIEVYDI QYLFYVLKKE VMDIADLPNE HINKSITDNL FVNDFFEYHS GISCQFHEDN
     DRGKYCTQSK VARWCYMFSD YMCGDLAIKP LPGKHMPPKQ EPKFRVINPE EPIENRCFSG
     MTTTEKSSGN TSFASISSLQ TEVKHEIVDH SAFSANLNAS EDFPSLGGRK GKRNAEPLAK
     GVWNVPASNC SVKDLGDAFN MPRR