ID   MAEL_PIG                Reviewed;         434 AA.
AC   A4UTQ0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Protein maelstrom homolog;
GN   Name=MAEL;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=18258292; DOI=10.1016/j.theriogenology.2007.11.020;
RA   Gau B.-H., Chu I.-M., Huang M.-C., Yang K.-T., Chiou S.-H., Fan Y.-H.,
RA   Chen M.-Y., Lin J.-H., Chuang C.-K., Huang S.-Y., Lee W.-C.;
RT   "Transcripts of enriched germ cells responding to heat shock as potential
RT   markers for porcine semen quality.";
RL   Theriogenology 69:758-766(2008).
CC   -!- FUNCTION: Plays a central role during spermatogenesis by repressing
CC       transposable elements and preventing their mobilization, which is
CC       essential for the germline integrity. Acts via the piRNA metabolic
CC       process, which mediates the repression of transposable elements during
CC       meiosis by forming complexes composed of piRNAs and Piwi proteins and
CC       governs the methylation and subsequent repression of transposons. Its
CC       association with piP-bodies suggests a participation in the secondary
CC       piRNAs metabolic process. Required for the localization of germ-cell
CC       factors to the meiotic nuage (By similarity).
CC       {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SUBUNIT: Interacts with SMARCB1, SIN3B and DDX4. Interacts with piRNA-
CC       associated proteins TDRD1, PIWIL1 and PIWIL2 (By similarity). Interacts
CC       with TEX19 (By similarity). {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BVN9}. Nucleus
CC       {ECO:0000250|UniProtKB:Q8BVN9}. Note=Component of the meiotic nuage,
CC       also named P granule, a germ-cell-specific organelle required to
CC       repress transposon activity during meiosis. Specifically localizes to
CC       piP-bodies, a subset of the nuage which contains secondary piRNAs (By
CC       similarity). {ECO:0000250|UniProtKB:Q8BVN9}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
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DR   EMBL; EF486859; ABO87660.1; -; mRNA.
DR   RefSeq; NP_001090980.1; NM_001097511.1.
DR   AlphaFoldDB; A4UTQ0; -.
DR   SMR; A4UTQ0; -.
DR   STRING; 9823.ENSSSCP00000023309; -.
DR   PaxDb; A4UTQ0; -.
DR   PRIDE; A4UTQ0; -.
DR   GeneID; 100048945; -.
DR   KEGG; ssc:100048945; -.
DR   CTD; 84944; -.
DR   eggNOG; ENOG502QTQB; Eukaryota.
DR   InParanoid; A4UTQ0; -.
DR   OrthoDB; 859881at2759; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043186; C:P granule; ISS:UniProtKB.
DR   GO; GO:0071547; C:piP-body; ISS:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0043046; P:DNA methylation involved in gamete generation; ISS:UniProtKB.
DR   GO; GO:0031047; P:gene silencing by RNA; ISS:UniProtKB.
DR   GO; GO:0007140; P:male meiotic nuclear division; IBA:GO_Central.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0034587; P:piRNA metabolic process; ISS:UniProtKB.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR024970; Maelstrom.
DR   InterPro; IPR039259; Protein_maelstrom.
DR   PANTHER; PTHR21358; PTHR21358; 1.
DR   Pfam; PF09011; HMG_box_2; 1.
DR   Pfam; PF13017; Maelstrom; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Differentiation; DNA-binding; Meiosis;
KW   Nucleus; Reference proteome; RNA-mediated gene silencing; Spermatogenesis.
FT   CHAIN           1..434
FT                   /note="Protein maelstrom homolog"
FT                   /id="PRO_0000367291"
FT   DNA_BIND        4..73
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          357..387
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   434 AA;  49147 MW;  2D65C6139FE24F1E CRC64;
     MPNRRGSRNA YYFFVQEKIP ELRRRGLPVA RVADAIPYCS ADWALLREEE KEKYSEMARE
     WKAAQGKDAG PWEKQKPVST PLRRPAMLVP KQNVSPPDMS CLSLKSDQAL LGGIFYFLNI
     FSHGELPPHC EQRFLPCEIG CVKYSLQEGI MADFHSFINP GEIPRGFRFH CQAASDSSHK
     IPISHFESGH DQATVLQNLY RFIRPNSGKW PPIYCKSDDR ARVNWCLKYM AKSSEITQDL
     ELVTVEDLVV GIYQQKFLKE PSKTWVRSLL DVAMWDYSSN TRCKWHEEND ILFCALAVCR
     KIAYCISNSL ATLLGIQLTE AHVPLQDYET SNSVTPKMVV LDAGRYQKLR VESSGFSHFS
     SSNQEQRSNT PTGDYPSGVK ISGQSSSVRG RGITRLLESI SNSSSNIHKF STCESSLSPY
     MSQKDGYKSF SSLS