MAEN_BACSU
ID MAEN_BACSU Reviewed; 448 AA.
AC O05256;
DT 22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Na(+)-malate symporter;
DE AltName: Full=Sodium-dependent malate transporter;
GN Name=maeN; Synonyms=yufR; OrderedLocusNames=BSU31580;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9274030; DOI=10.1099/00221287-143-8-2769;
RA Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H.,
RA Duesterhoeft A., Pohl T.M., Weitzenegger T.;
RT "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence
RT of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).";
RL Microbiology 143:2769-2774(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RX PubMed=10903309; DOI=10.1074/jbc.m001112200;
RA Wei Y., Guffanti A.A., Ito M., Krulwich T.A.;
RT "Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that
RT enhances growth on malate at low protonmotive force.";
RL J. Biol. Chem. 275:30287-30292(2000).
RN [4]
RP REGULATION BY MALK/MALR.
RX PubMed=12949159; DOI=10.1099/mic.0.26257-0;
RA Tanaka K., Kobayashi K., Ogasawara N.;
RT "The Bacillus subtilis YufLM two-component system regulates the expression
RT of the malate transporters MaeN (YufR) and YflS, and is essential for
RT utilization of malate in minimal medium.";
RL Microbiology 149:2317-2329(2003).
CC -!- FUNCTION: Acts as a Na(+)-malate symporter, as it catalyzes malate-
CC dependent uptake of Na(+) and Na(+)-dependent uptake of malate.
CC {ECO:0000269|PubMed:10903309}.
CC -!- ACTIVITY REGULATION: Activated by the two-component regulatory system
CC MalK/MalR in response to malate. The regulator MalR binds to the
CC promoter region of maeN.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sodium:citrate (SCF) symporter family.
CC {ECO:0000305}.
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DR EMBL; Z93937; CAB07940.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15147.1; -; Genomic_DNA.
DR PIR; G70009; G70009.
DR RefSeq; NP_391036.1; NC_000964.3.
DR RefSeq; WP_003244241.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O05256; -.
DR SMR; O05256; -.
DR STRING; 224308.BSU31580; -.
DR TCDB; 2.A.24.2.3; the 2-hydroxycarboxylate transporter (2-hct) family.
DR PaxDb; O05256; -.
DR PRIDE; O05256; -.
DR EnsemblBacteria; CAB15147; CAB15147; BSU_31580.
DR GeneID; 938855; -.
DR KEGG; bsu:BSU31580; -.
DR PATRIC; fig|224308.43.peg.3306; -.
DR eggNOG; COG3493; Bacteria.
DR InParanoid; O05256; -.
DR OMA; NMGGTGD; -.
DR PhylomeDB; O05256; -.
DR BioCyc; BSUB:BSU31580-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR018025; 2-OHcarbox_trans_Prot/Firm.
DR InterPro; IPR004679; 2-OHcarboxylate_transport.
DR PANTHER; PTHR40033; PTHR40033; 1.
DR Pfam; PF03390; 2HCT; 1.
DR PIRSF; PIRSF005348; YxkH; 1.
DR TIGRFAMs; TIGR00783; ccs; 1.
PE 3: Inferred from homology;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..448
FT /note="Na(+)-malate symporter"
FT /id="PRO_0000088762"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 448 AA; 47824 MW; 74F495E7735512E9 CRC64;
MGAIPKTGTI SPEQKDSQEK NLFQKIWSWE IGVIPLPLYT VLAVIIILAA YYNELPANML
GGFAIIMILG VFLGDIGQRI PILKDIGGPA ILSLFVPSFL VFYNVLNSTS LDAVTNLMKT
SNFLYFYIAC LVVGSILGMN RIVLIQGFIR MFVPLVAGTI AAVAAGILVG FIFGYSAYDS
FFFVVVPIIA GGIGEGILPL SIAYSQILGS SADVFVSQLV PAAIIGNVFA IICAALMKKL
GDKRPDLNGN GRLVKSKKAN EIFNQKEAEA KIDFKLMGAG VLLACTFFIF GGLLEKFIFI
PGAILMIISA AAVKYANILP KKMEEGAYQL YKFISSSFTW PLMVGLGILF IPLDDVASVI
SIPFVIICIS VVIAMIGSGY FVGKLMNMYP VESAIVTCCH SGLGGTGDVA ILSASGRMGL
MPFAQISTRL GGAGTVICAT VLLRFFTS
