MAF5_ARATH
ID MAF5_ARATH Reviewed; 198 AA.
AC Q683D7; C0J8N7; F4KGI3; Q84NA6; Q84NA7; Q9FJQ7; Q9FQX6;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Protein MADS AFFECTING FLOWERING 5;
DE AltName: Full=MADS box protein FCL1;
DE AltName: Full=MADS-box protein AGAMOUS-LIKE 68;
GN Name=MAF5; Synonyms=AGL68, FCL1; OrderedLocusNames=At5g65080;
GN ORFNames=F15O5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND GENE FAMILY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=12837945; DOI=10.1105/tpc.011544;
RA Parenicova L., de Folter S., Kieffer M., Horner D.S., Favalli C.,
RA Busscher J., Cook H.E., Ingram R.M., Kater M.M., Davies B., Angenent G.C.,
RA Colombo L.;
RT "Molecular and phylogenetic analyses of the complete MADS-box transcription
RT factor family in Arabidopsis: new openings to the MADS world.";
RL Plant Cell 15:1538-1551(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (IOFORMS 1 AND 2), FUNCTION, AND INDUCTION BY
RP VERNALIZATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=12724541; DOI=10.1105/tpc.009506;
RA Ratcliffe O.J., Kumimoto R.W., Wong B.J., Riechmann J.L.;
RT "Analysis of the Arabidopsis MADS AFFECTING FLOWERING gene family: MAF2
RT prevents vernalization by short periods of cold.";
RL Plant Cell 15:1159-1169(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=19139056; DOI=10.1093/molbev/msn300;
RA Caicedo A.L., Richards C., Ehrenreich I.M., Purugganan M.D.;
RT "Complex rearrangements lead to novel chimeric gene fusion polymorphisms at
RT the Arabidopsis thaliana MAF2-5 flowering time gene cluster.";
RL Mol. Biol. Evol. 26:699-711(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-198 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Schlappi M.R.;
RT "FCL1 is an expressed member of small MADS box genes in Arabidopsis and has
RT homology to FLC.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [10]
RP FUNCTION.
RX PubMed=18852898; DOI=10.1371/journal.pone.0003404;
RA Jiang D., Wang Y., Wang Y., He Y.;
RT "Repression of FLOWERING LOCUS C and FLOWERING LOCUS T by the Arabidopsis
RT Polycomb repressive complex 2 components.";
RL PLoS ONE 3:E3404-E3404(2008).
RN [11]
RP FUNCTION.
RX PubMed=18798874; DOI=10.1111/j.1365-313x.2008.03684.x;
RA Xu L., Menard R., Berr A., Fuchs J., Cognat V., Meyer D., Shen W.H.;
RT "The E2 ubiquitin-conjugating enzymes, AtUBC1 and AtUBC2, play redundant
RT roles and are involved in activation of FLC expression and repression of
RT flowering in Arabidopsis thaliana.";
RL Plant J. 57:279-288(2009).
RN [12]
RP FUNCTION.
RX PubMed=21150261; DOI=10.4161/psb.5.12.14035;
RA Kim D.-H., Sung S.;
RT "Role of VIN3-LIKE 2 in facultative photoperiodic flowering response in
RT Arabidopsis.";
RL Plant Signal. Behav. 5:1672-1673(2010).
RN [13]
RP FUNCTION.
RX PubMed=20837520; DOI=10.1073/pnas.1010834107;
RA Kim D.-H., Sung S.;
RT "The plant homeo domain finger protein, VIN3-LIKE 2, is necessary for
RT photoperiod-mediated epigenetic regulation of the floral repressor, MAF5.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:17029-17034(2010).
RN [14]
RP FUNCTION.
RX PubMed=21175890; DOI=10.1111/j.1365-313x.2010.04402.x;
RA Yoo S.K., Wu X., Lee J.S., Ahn J.H.;
RT "AGAMOUS-LIKE 6 is a floral promoter that negatively regulates the FLC/MAF
RT clade genes and positively regulates FT in Arabidopsis.";
RL Plant J. 65:62-76(2011).
RN [15]
RP FUNCTION.
RX PubMed=21398257; DOI=10.1104/pp.111.174417;
RA Yu C.-W., Liu X., Luo M., Chen C., Lin X., Tian G., Lu Q., Cui Y., Wu K.;
RT "HISTONE DEACETYLASE6 interacts with FLOWERING LOCUS D and regulates
RT flowering in Arabidopsis.";
RL Plant Physiol. 156:173-184(2011).
RN [16]
RP FUNCTION.
RX PubMed=22378382; DOI=10.1093/pcp/pcs021;
RA Yun J.-Y., Tamada Y., Kang Y.E., Amasino R.M.;
RT "Arabidopsis trithorax-related3/SET domain GROUP2 is required for the
RT winter-annual habit of Arabidopsis thaliana.";
RL Plant Cell Physiol. 53:834-846(2012).
CC -!- FUNCTION: Transcription factor involved in the negative regulation of
CC flowering time in short days, probably through the photoperiodic and
CC vernalization pathways. Prevents premature flowering, particularly in
CC the cv. Landsberg erecta background. In non-inductive photoperiods
CC (e.g. short days), required for flowering through VIL2-mediated
CC maintenance of the epigenetically repressed state of MAF5 via H3K9me2
CC and plant homeodomain / polycomb repressive complex 2 (PHD-PRC2)-
CC dependent H3K27me3. {ECO:0000269|PubMed:12724541,
CC ECO:0000269|PubMed:18798874, ECO:0000269|PubMed:18852898,
CC ECO:0000269|PubMed:20837520, ECO:0000269|PubMed:21150261,
CC ECO:0000269|PubMed:21175890, ECO:0000269|PubMed:21398257,
CC ECO:0000269|PubMed:22378382}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q683D7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q683D7-2; Sequence=VSP_046574;
CC Name=3;
CC IsoId=Q683D7-3; Sequence=VSP_046573;
CC -!- INDUCTION: Up-regulated by vernalization. Repressed by VIL2, AGL6, CLF,
CC EMF2 and FIE via epigenetic chromatin H3K27me3 and H3K9me2 regulation
CC during the vegetative development. {ECO:0000269|PubMed:12724541}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF19021.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ACL93445.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB11644.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD42943.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY141219; AAN52783.1; -; mRNA.
DR EMBL; AY231455; AAO65320.1; -; mRNA.
DR EMBL; AY231456; AAO65321.1; -; mRNA.
DR EMBL; EU980626; ACL93445.1; ALT_INIT; Genomic_DNA.
DR EMBL; AB013395; BAB11644.1; ALT_INIT; Genomic_DNA.
DR EMBL; AB026633; BAB11644.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK175180; BAD42943.1; ALT_INIT; mRNA.
DR EMBL; BT025268; ABF19021.1; ALT_INIT; mRNA.
DR EMBL; AF214485; AAG43854.1; -; mRNA.
DR RefSeq; NP_001078799.2; NM_001085330.2.
DR AlphaFoldDB; Q683D7; -.
DR SMR; Q683D7; -.
DR BioGRID; 21874; 10.
DR DIP; DIP-60817N; -.
DR IntAct; Q683D7; 1.
DR STRING; 3702.AT5G65080.1; -.
DR PaxDb; Q683D7; -.
DR ProteomicsDB; 238625; -. [Q683D7-1]
DR GeneID; 836632; -.
DR KEGG; ath:AT5G65080; -.
DR Araport; AT5G65080; -.
DR TAIR; locus:2144355; AT5G65080.
DR eggNOG; KOG0014; Eukaryota.
DR HOGENOM; CLU_053053_0_4_1; -.
DR InParanoid; Q683D7; -.
DR OrthoDB; 1609924at2759; -.
DR PRO; PR:Q683D7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q683D7; baseline and differential.
DR Genevisible; Q683D7; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0010582; P:floral meristem determinacy; IBA:GO_Central.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IGI:UniProtKB.
DR GO; GO:0048587; P:regulation of short-day photoperiodism, flowering; IGI:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010048; P:vernalization response; IEP:TAIR.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR002487; TF_Kbox.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF01486; K-box; 1.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS51297; K_BOX; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..198
FT /note="Protein MADS AFFECTING FLOWERING 5"
FT /id="PRO_0000422715"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT DOMAIN 80..173
FT /note="K-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT REGION 175..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 62..81
FT /note="SMAKIISRFKIQQADDPETL -> RVVYVSWKRKNFTIFLSWQ (in
FT isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_046573"
FT VAR_SEQ 150..163
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046574"
FT CONFLICT 102
FT /note="R -> S (in Ref. 9; AAG43854)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 198 AA; 22343 MW; 2B525B7EF169AF2F CRC64;
MGRRRVEIKR IENKSSRQVT FCKRRNGLME KARQLSILCG SSVALFIVSS TGKLYNSSSG
DSMAKIISRF KIQQADDPET LDLEDKTQDY LSHKELLEIV QRKIEEAKGD NVSIESLISM
EEQLKSALSV IRARKTELLM ELVKNLQDKE KLLKEKNKVL ASEVGKLKKI LETGDERAVM
SPENSSGHSP PETLPLLK
