ID   MAFA_NEIMB              Reviewed;         313 AA.
AC   Q9JS44;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Adhesin MafA;
DE   Flags: Precursor;
GN   Name=mafA1; OrderedLocusNames=NMB0375;
GN   and
GN   Name=mafA2; OrderedLocusNames=NMB0652;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [2]
RP   INDUCTION.
RC   STRAIN=MC58;
RX   PubMed=12172557; DOI=10.1038/nbt728;
RA   Grifantini R., Bartolini E., Muzzi A., Draghi M., Frigimelica E.,
RA   Berger J., Ratti G., Petracca R., Galli G., Agnusdei M., Giuliani M.M.,
RA   Santini L., Brunelli B., Tettelin H., Rappuoli R., Randazzo F., Grandi G.;
RT   "Previously unrecognized vaccine candidates against group B meningococcus
RT   identified by DNA microarrays.";
RL   Nat. Biotechnol. 20:914-921(2002).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17038831; DOI=10.4161/hv.1.2.1651;
RA   Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J.;
RT   "Characterization of the protein content of a meningococcal outer membrane
RT   vesicle vaccine by polyacrylamide gel electrophoresis and mass
RT   spectrometry.";
RL   Hum. Vaccin. 1:80-84(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=NZ98/254 / Serogroup B;
RX   PubMed=16645985; DOI=10.1002/pmic.200500821;
RA   Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.;
RT   "Proteomic analysis of a meningococcal outer membrane vesicle vaccine
RT   prepared from the group B strain NZ98/254.";
RL   Proteomics 6:3400-3413(2006).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Induced during interaction with human epithelial cells.
CC       {ECO:0000269|PubMed:12172557}.
CC   -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC       used as vaccines in human.
CC   -!- SIMILARITY: Belongs to the MafA family. {ECO:0000305}.
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DR   EMBL; AE002098; AAF41073.1; -; Genomic_DNA.
DR   EMBL; AE002098; AAF62309.1; -; Genomic_DNA.
DR   PIR; G81173; G81173.
DR   RefSeq; NP_273424.1; NC_003112.2.
DR   RefSeq; NP_273694.1; NC_003112.2.
DR   RefSeq; WP_002222000.1; NC_003112.2.
DR   AlphaFoldDB; Q9JS44; -.
DR   PaxDb; Q9JS44; -.
DR   EnsemblBacteria; AAF41073; AAF41073; NMB0652.
DR   EnsemblBacteria; AAF62309; AAF62309; NMB0375.
DR   KEGG; nme:NMB0375; -.
DR   KEGG; nme:NMB0652; -.
DR   PATRIC; fig|122586.8.peg.472; -.
DR   HOGENOM; CLU_985210_0_0_4; -.
DR   OMA; TKNNGRK; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell adhesion; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Virulence.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           15..313
FT                   /note="Adhesin MafA"
FT                   /id="PRO_0000344487"
FT   REGION          282..313
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        294..313
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           15
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           15
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   313 AA;  34000 MW;  360676272DAD60C1 CRC64;
     MKTLLLLIPL VLTACGTLTG IPAHGGGKRF AVEQELVAAS SRAAVKEMDL SALKGRKAAL
     YVSVMGDQGS GNISGGRYSI DALIRGGYHN NPESATQYSY PAYDTTATTK SDALSSVTTS
     TSLLNAPAAA LTKNSGRKGE RSAGLSVNGT GDYRNETLLA NPRDVSFLTN LIQTVFYLRG
     IEVVPPEYAD TDVFVTVDVF GTVRSRTELH LYNAETLKAQ TKLEYFAVDR DSRKLLITPK
     TAAYESQYQE QYALWTGPYK VSKTVKASDR LMVDFSDITP YGDTTAQNRP DFKQNNGKKP
     DVGNEVIRRR KGG