MAG2_YEAST
ID MAG2_YEAST Reviewed; 670 AA.
AC Q06436; D6VZ63;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=RING-finger protein MAG2;
GN Name=MAG2; OrderedLocusNames=YLR427W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 922 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RNF10 family. {ECO:0000305}.
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DR EMBL; U20939; AAB67504.1; -; Genomic_DNA.
DR EMBL; AY692728; AAT92747.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09729.1; -; Genomic_DNA.
DR PIR; S53414; S53414.
DR RefSeq; NP_013531.3; NM_001182315.3.
DR AlphaFoldDB; Q06436; -.
DR BioGRID; 31687; 77.
DR DIP; DIP-4633N; -.
DR IntAct; Q06436; 35.
DR STRING; 4932.YLR427W; -.
DR iPTMnet; Q06436; -.
DR MaxQB; Q06436; -.
DR PaxDb; Q06436; -.
DR PRIDE; Q06436; -.
DR EnsemblFungi; YLR427W_mRNA; YLR427W; YLR427W.
DR GeneID; 851147; -.
DR KEGG; sce:YLR427W; -.
DR SGD; S000004419; MAG2.
DR VEuPathDB; FungiDB:YLR427W; -.
DR eggNOG; KOG2164; Eukaryota.
DR GeneTree; ENSGT00390000001731; -.
DR HOGENOM; CLU_011811_0_0_1; -.
DR InParanoid; Q06436; -.
DR OMA; PCLLHYL; -.
DR BioCyc; YEAST:G3O-32487-MON; -.
DR PRO; PR:Q06436; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06436; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039739; Mag2/Rnf10.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR12983; PTHR12983; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..670
FT /note="RING-finger protein MAG2"
FT /id="PRO_0000257809"
FT ZN_FING 195..250
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 1..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 609..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..74
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..637
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 670 AA; 76317 MW; 88194FC215161E03 CRC64;
MVEPDMQKKA SGGSGGSEMD TLNATSNSSK QGVSNNKRNP VSKKKPGNKV SDGRDNAHNY
HGEGRRKSSK QQRSRTPYKE TSTRINDQDI DLSIQEEILG GNFKLRGRKT QVSINHLLNF
QLPEVEREKS RSSSSKKSNR RRDEHVHLHG DTFVNVNYRL LVDDRFDYPE QNCNPNVPVD
QEKILRVIVP KGQNCSICLS EEPVAPRMVT CGHIFCLSCL LNFFSIEETV KNKETGYSKK
KKYKECPLCG SIIGPKRVKP VLYEDDFDVT RLNQKPEPGA TVHLQLMCKP HGSLLPLPVA
LHLDPLKCGN FPPANLGSIK HYAHIMKCGV SYSLELYQKD IVAIQEQYEI DKAIYNDSGK
FVKQSIENIN DQISTLLAAT TDLSPLSNDI NNGLDNFHFD DDLLTKYDDS SAYFFYQTLV
ASSTKYFLSP LDVKILLTIF HYYSKFPESI ETTVENIHYD TVVTEQLIRR YKYIGHLPIG
TEIALLDLDW RKIPFLPKEI YEQFAHELKQ RRRKFTMKKQ KEDKEKKLYE KRLEQEHAEF
YRKENGNSLK FEDSVQMATH YESIVSSSIP LNSLGISMLG PPTNSCSTPQ KQAPSHTKRT
IWGTSIAVTE DEKASKENKE FQDMLLQRIR QEDSSDVTDS TDSPPTSNGK RGRKKKGKVM
LFSSNHQALG
