MAGB2_HUMAN
ID MAGB2_HUMAN Reviewed; 319 AA.
AC O15479; O75860;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 3.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Melanoma-associated antigen B2;
DE AltName: Full=Cancer/testis antigen 3.2;
DE Short=CT3.2;
DE AltName: Full=DSS-AHC critical interval MAGE superfamily 6;
DE Short=DAM6;
DE AltName: Full=MAGE XP-2 antigen;
DE AltName: Full=MAGE-B2 antigen;
GN Name=MAGEB2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8535061; DOI=10.1007/bf00352360;
RA Dabovic B., Zanaria E., Bardoni B., Lisa A., Bordignon C., Russo V.,
RA Matessi C., Traversari C., Camerino G.;
RT "A family of rapidly evolving genes from the sex reversal critical region
RT in Xp21.";
RL Mamm. Genome 6:571-580(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LYS-61.
RX PubMed=9441743; DOI=10.1006/geno.1997.5052;
RA Lurquin C., De Smet C., Brasseur F., Muscatelli F., Martelange V.,
RA De Plaen E., Brasseur R., Monaco A.P., Boon T.;
RT "Two members of the human MAGEB gene family located in Xp21.3 are expressed
RT in tumors of various histological origins.";
RL Genomics 46:397-408(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT LYS-61.
RX PubMed=9538511; DOI=10.1006/mgme.1997.2639;
RA McCurdy D.K., Tai L.-Q., Nguyen J., Wang Z., Yang H.M., Udar N., Naiem F.,
RA Concannon P., Gatti R.A.;
RT "MAGE Xp-2: a member of the MAGE gene family isolated from an expression
RT library using systemic lupus erythematosus sera.";
RL Mol. Genet. Metab. 63:3-13(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LYS-61.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-105, VARIANT
RP [LARGE SCALE ANALYSIS] LYS-61, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP FUNCTION, AND INTERACTION WITH TRIM28.
RX PubMed=20864041; DOI=10.1016/j.molcel.2010.08.029;
RA Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.;
RT "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.";
RL Mol. Cell 39:963-974(2010).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-105, VARIANT
RP [LARGE SCALE ANALYSIS] LYS-61, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: May enhance ubiquitin ligase activity of RING-type zinc
CC finger-containing E3 ubiquitin-protein ligases. Proposed to act through
CC recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at
CC the E3:substrate complex. {ECO:0000269|PubMed:20864041}.
CC -!- SUBUNIT: Interacts with TRIM28. {ECO:0000269|PubMed:20864041}.
CC -!- INTERACTION:
CC O15479; Q99856: ARID3A; NbExp=3; IntAct=EBI-1057615, EBI-5458244;
CC O15479; Q96S94-5: CCNL2; NbExp=3; IntAct=EBI-1057615, EBI-12024864;
CC O15479; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-1057615, EBI-742054;
CC O15479; Q9BQ95: ECSIT; NbExp=3; IntAct=EBI-1057615, EBI-712452;
CC O15479; P35637: FUS; NbExp=3; IntAct=EBI-1057615, EBI-400434;
CC O15479; Q96KN7: RPGRIP1; NbExp=3; IntAct=EBI-1057615, EBI-1050213;
CC O15479; Q99757: TXN2; NbExp=3; IntAct=EBI-1057615, EBI-2932492;
CC O15479; O96006: ZBED1; NbExp=3; IntAct=EBI-1057615, EBI-740037;
CC -!- TISSUE SPECIFICITY: Expressed in testis and placenta, and in a
CC significant fraction of tumors of various histologic types.
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DR EMBL; U93163; AAC23617.1; -; Genomic_DNA.
DR EMBL; AF015766; AAD01565.1; -; mRNA.
DR EMBL; AC005185; AAD10635.1; -; Genomic_DNA.
DR EMBL; BC026071; AAH26071.1; -; mRNA.
DR CCDS; CCDS14219.1; -.
DR RefSeq; NP_002355.2; NM_002364.4.
DR RefSeq; XP_011543814.1; XM_011545512.1.
DR AlphaFoldDB; O15479; -.
DR SMR; O15479; -.
DR BioGRID; 110287; 340.
DR IntAct; O15479; 24.
DR MINT; O15479; -.
DR STRING; 9606.ENSP00000368273; -.
DR iPTMnet; O15479; -.
DR PhosphoSitePlus; O15479; -.
DR BioMuta; MAGEB2; -.
DR EPD; O15479; -.
DR jPOST; O15479; -.
DR MassIVE; O15479; -.
DR MaxQB; O15479; -.
DR PaxDb; O15479; -.
DR PeptideAtlas; O15479; -.
DR PRIDE; O15479; -.
DR ProteomicsDB; 48684; -.
DR Antibodypedia; 24654; 199 antibodies from 27 providers.
DR DNASU; 4113; -.
DR Ensembl; ENST00000378988.5; ENSP00000368273.4; ENSG00000099399.6.
DR GeneID; 4113; -.
DR KEGG; hsa:4113; -.
DR MANE-Select; ENST00000378988.5; ENSP00000368273.4; NM_002364.5; NP_002355.2.
DR UCSC; uc004dbz.4; human.
DR CTD; 4113; -.
DR DisGeNET; 4113; -.
DR GeneCards; MAGEB2; -.
DR HGNC; HGNC:6809; MAGEB2.
DR HPA; ENSG00000099399; Tissue enriched (testis).
DR MIM; 300098; gene.
DR neXtProt; NX_O15479; -.
DR OpenTargets; ENSG00000099399; -.
DR PharmGKB; PA30555; -.
DR VEuPathDB; HostDB:ENSG00000099399; -.
DR eggNOG; KOG4562; Eukaryota.
DR GeneTree; ENSGT00940000155485; -.
DR HOGENOM; CLU_039582_1_0_1; -.
DR InParanoid; O15479; -.
DR OMA; ECLLSTW; -.
DR OrthoDB; 1195799at2759; -.
DR PhylomeDB; O15479; -.
DR TreeFam; TF328505; -.
DR PathwayCommons; O15479; -.
DR SignaLink; O15479; -.
DR BioGRID-ORCS; 4113; 4 hits in 704 CRISPR screens.
DR ChiTaRS; MAGEB2; human.
DR GeneWiki; MAGEB2; -.
DR GenomeRNAi; 4113; -.
DR Pharos; O15479; Tbio.
DR PRO; PR:O15479; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; O15479; protein.
DR Bgee; ENSG00000099399; Expressed in right testis and 32 other tissues.
DR Genevisible; O15479; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.1200; -; 1.
DR Gene3D; 1.10.10.1210; -; 1.
DR InterPro; IPR037445; MAGE.
DR InterPro; IPR021072; MAGE_N.
DR InterPro; IPR041898; MAGE_WH1.
DR InterPro; IPR041899; MAGE_WH2.
DR InterPro; IPR030084; MAGEB2.
DR InterPro; IPR002190; MHD_dom.
DR PANTHER; PTHR11736; PTHR11736; 1.
DR PANTHER; PTHR11736:SF152; PTHR11736:SF152; 1.
DR Pfam; PF01454; MAGE; 1.
DR Pfam; PF12440; MAGE_N; 1.
DR SMART; SM01373; MAGE; 1.
DR SMART; SM01392; MAGE_N; 1.
DR PROSITE; PS50838; MAGE; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Tumor antigen; Ubl conjugation pathway.
FT CHAIN 1..319
FT /note="Melanoma-associated antigen B2"
FT /id="PRO_0000156713"
FT DOMAIN 111..310
FT /note="MAGE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT REGION 1..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 77
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 105
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT VARIANT 61
FT /note="E -> K (in dbSNP:rs2529541)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:9441743, ECO:0000269|PubMed:9538511,
FT ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163"
FT /id="VAR_027675"
FT VARIANT 318
FT /note="G -> R (in dbSNP:rs5972090)"
FT /id="VAR_027676"
SQ SEQUENCE 319 AA; 35277 MW; CF0BD27A832D5CBB CRC64;
MPRGQKSKLR AREKRRKARD ETRGLNVPQV TEAEEEEAPC CSSSVSGGAA SSSPAAGIPQ
EPQRAPTTAA AAAAGVSSTK SKKGAKSHQG EKNASSSQAS TSTKSPSEDP LTRKSGSLVQ
FLLYKYKIKK SVTKGEMLKI VGKRFREHFP EILKKASEGL SVVFGLELNK VNPNGHTYTF
IDKVDLTDEE SLLSSWDFPR RKLLMPLLGV IFLNGNSATE EEIWEFLNML GVYDGEEHSV
FGEPWKLITK DLVQEKYLEY KQVPSSDPPR FQFLWGPRAY AETSKMKVLE FLAKVNGTTP
CAFPTHYEEA LKDEEKAGV
